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公开(公告)号:US20220282150A1
公开(公告)日:2022-09-08
申请号:US17633427
申请日:2020-08-06
IPC分类号: C09K11/02 , G01N33/531 , G01N33/543 , C09K11/06
摘要: A light-emitting marker having a light-emitting core comprising a light-emitting material bound to a first biotin group and a biomolecule bound to a second biotin group. A protein, e.g. streptavidin or neutravidin, is bound to the first and second biotin groups. The light-emitting marker may be a light-emitting marker particle having a particulate core.
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公开(公告)号:US20230303762A1
公开(公告)日:2023-09-28
申请号:US18020053
申请日:2021-08-06
发明人: Kiran Kamtekar , Nazrul Islam , Sheena Zuberi , Jonathan Behrendt
CPC分类号: C08G61/124 , C09K11/06 , G01N33/582 , G01N21/6428 , C09K2211/1491 , G01N2021/6439
摘要: A conjugated light-emitting polymer comprising a host repeat unit and an intermediate repeat unit in a backbone of the conjugated light emitting polymer, and an emissive unit. The host repeat unit has a wider band gap than the intermediate unit. The intermediate repeat unit has a wider band gap than the emissive unit. The emissive unit is either: an emissive repeat unit of a block copolymer wherein the block copolymer comprises a first block comprising the intermediate repeat unit and the emissive repeat unit and a second block comprising the host repeat unit; a substituent of a proportion of the host repeat units and at least some of the host repeat units substituted with an emissive unit are arranged directly adjacent to an intermediate repeat unit; a substituent of a proportion of the intermediate repeat units; or a repeat unit in the backbone of the conjugated polymer and is arranged directly adjacent to the intermediate repeat unit. The conjugated light-emitting polymer may be part of a light-emitting marker for detecting the presence of a target analyte in a sample.
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公开(公告)号:US20240124767A1
公开(公告)日:2024-04-18
申请号:US17767415
申请日:2020-10-08
发明人: Jonathan Behrendt , Kiran Kamtekar , Nazrul Islam
IPC分类号: C09K11/06 , C09K11/02 , G01N15/1434
CPC分类号: C09K11/06 , C09K11/02 , G01N15/1434 , C09K2211/1483 , C09K2211/1491 , C09K2211/185
摘要: A light-emitting composition containing first and second light-emitting markers. The first light-emitting marker comprises a first light-emitting material and a first binding group configured to bind to a first target analyte. The second light-emitting marker comprises a second light-emitting material which is different from the first light-emitting material and a second binding group which is different from the first binding group and which is configured to bind to a second target analyte. A luminescent lifetime of the first light-emitting material is shorter than a luminescent lifetime of the second light-emitting material. The difference in lifetimes of the first and second light-emitting materials may be used to distinguish between the first and second target analytes in a sample, e.g. by time-gated flow cytometry.
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公开(公告)号:US20230152328A1
公开(公告)日:2023-05-18
申请号:US17916345
申请日:2021-03-30
发明人: Sheena Zuberi , Nazrul Islam , Jonathan Behrendt
CPC分类号: G01N33/582 , C09K11/06 , C08G61/126
摘要: A target analyte in a sample may be identified using a light-emitting ting marker is irradiated and emission from the sample at an emission wavelength of the light-emitting marker is detected. The method may be flow cytometry. The light-emitting marker comprises a light-emitting polymer comprising an electron-accepting repeat unit of formula (I) and an electron-donating repeat unit: (I) R1 and R2 independently in each occurrence is H or a substituent and X in each occurrence is dependently a substituent. The light-emitting marker may be a particulate light-emitting marker comprising particles containing the light-emitting polymer and a matrix material, e.g. silica.
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公开(公告)号:US20230093363A1
公开(公告)日:2023-03-23
申请号:US17909251
申请日:2021-03-02
发明人: Jonathan Behrendt
摘要: A light-emitting particle comprising a core and a composite shell layer in contact with and surrounding the core wherein the composite shell layer comprises silica and a light-emitting polymer distributed across the thickness of the composite shell layer.
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公开(公告)号:US20240117244A1
公开(公告)日:2024-04-11
申请号:US18467380
申请日:2023-09-14
发明人: Ruth Pegington , Jonathan Behrendt
CPC分类号: C09K11/025 , C09K11/06 , C09K2211/14
摘要: A method of treating a reaction mixture comprising light-emitting nanoparticles formed upon reaction of a silica-forming compound in the presence of a light-emitting material in a protic, water-miscible solvent. The method comprises forming a phase-separated system comprising: an aqueous phase comprising the protic, water miscible solvent, an organic phase comprising an organic solvent and a separating solvent; and separating the organic phase. The separating solvent is immiscible with water, miscible with the water-miscible protic solvent and miscible with the organic solvent.
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公开(公告)号:US20230374582A1
公开(公告)日:2023-11-23
申请号:US18031330
申请日:2021-10-12
IPC分类号: C12Q1/6869 , G01N33/58
CPC分类号: C12Q1/6869 , G01N33/582
摘要: A method of determining whether a test nucleotide comprises a base complementary to the next base of a template strand immediately downstream of a primer in a primed template nucleic acid molecule is described. The method comprises the use of a marked nucleotide comprising a test nucleotide conjugated to a light emitting marker by a linker.
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公开(公告)号:US20230176065A1
公开(公告)日:2023-06-08
申请号:US17916289
申请日:2021-03-31
发明人: Sheena Zuberi , Nazrul Islam , Jonathan Behrendt , Kiran Kamtekar
CPC分类号: G01N33/582 , C09K11/06 , G01N21/6428 , C09K2211/1483 , G01N2021/6439
摘要: A method of identifying a target analyte in a sample containing a light-emitting marker configured to bind to the target analyte and detecting emission from the light-emitting marker. The light-emitting marker comprises a light-emitting polymer comprising a repeat unit of formula (I).
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公开(公告)号:US20230102078A1
公开(公告)日:2023-03-30
申请号:US17909243
申请日:2021-03-02
发明人: Jonathan Behrendt , Kiran Kamtekar , Nazrul Islam
IPC分类号: C09K11/02 , C09K11/06 , C12Q1/6869 , G01N33/58
摘要: A light-emitting particle comprising a core comprising a matrix material and a light-emitting system comprising a polymer and a shell layer comprising an inorganic oxide in contact with and surrounding the core.
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公开(公告)号:US20220195513A1
公开(公告)日:2022-06-23
申请号:US17555151
申请日:2021-12-17
IPC分类号: C12Q1/6869 , C12Q1/6809
摘要: A method for determining the sequence of a polynucleotide is described. The method comprises detecting in a sequencing reaction the incorporation of a first, second, third, or fourth nucleotide using first and second excitation wavelengths and a photodetector having a detection window comprising a range of wavelengths.
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