LIGHT-EMITTING MARKER
    2.
    发明公开

    公开(公告)号:US20230303762A1

    公开(公告)日:2023-09-28

    申请号:US18020053

    申请日:2021-08-06

    摘要: A conjugated light-emitting polymer comprising a host repeat unit and an intermediate repeat unit in a backbone of the conjugated light emitting polymer, and an emissive unit. The host repeat unit has a wider band gap than the intermediate unit. The intermediate repeat unit has a wider band gap than the emissive unit. The emissive unit is either: an emissive repeat unit of a block copolymer wherein the block copolymer comprises a first block comprising the intermediate repeat unit and the emissive repeat unit and a second block comprising the host repeat unit; a substituent of a proportion of the host repeat units and at least some of the host repeat units substituted with an emissive unit are arranged directly adjacent to an intermediate repeat unit; a substituent of a proportion of the intermediate repeat units; or a repeat unit in the backbone of the conjugated polymer and is arranged directly adjacent to the intermediate repeat unit. The conjugated light-emitting polymer may be part of a light-emitting marker for detecting the presence of a target analyte in a sample.

    LIGHT-EMITTING COMPOSITION
    3.
    发明公开

    公开(公告)号:US20240124767A1

    公开(公告)日:2024-04-18

    申请号:US17767415

    申请日:2020-10-08

    摘要: A light-emitting composition containing first and second light-emitting markers. The first light-emitting marker comprises a first light-emitting material and a first binding group configured to bind to a first target analyte. The second light-emitting marker comprises a second light-emitting material which is different from the first light-emitting material and a second binding group which is different from the first binding group and which is configured to bind to a second target analyte. A luminescent lifetime of the first light-emitting material is shorter than a luminescent lifetime of the second light-emitting material. The difference in lifetimes of the first and second light-emitting materials may be used to distinguish between the first and second target analytes in a sample, e.g. by time-gated flow cytometry.

    LIGHT-EMITTING MARKER
    4.
    发明公开

    公开(公告)号:US20230152328A1

    公开(公告)日:2023-05-18

    申请号:US17916345

    申请日:2021-03-30

    IPC分类号: G01N33/58 C09K11/06 C08G61/12

    摘要: A target analyte in a sample may be identified using a light-emitting ting marker is irradiated and emission from the sample at an emission wavelength of the light-emitting marker is detected. The method may be flow cytometry. The light-emitting marker comprises a light-emitting polymer comprising an electron-accepting repeat unit of formula (I) and an electron-donating repeat unit: (I) R1 and R2 independently in each occurrence is H or a substituent and X in each occurrence is dependently a substituent. The light-emitting marker may be a particulate light-emitting marker comprising particles containing the light-emitting polymer and a matrix material, e.g. silica.

    LIGHT-EMITTING PARTICLES
    5.
    发明申请

    公开(公告)号:US20230093363A1

    公开(公告)日:2023-03-23

    申请号:US17909251

    申请日:2021-03-02

    发明人: Jonathan Behrendt

    IPC分类号: C09K11/06 C09K11/02 G01N33/58

    摘要: A light-emitting particle comprising a core and a composite shell layer in contact with and surrounding the core wherein the composite shell layer comprises silica and a light-emitting polymer distributed across the thickness of the composite shell layer.

    METHOD
    6.
    发明公开
    METHOD 审中-公开

    公开(公告)号:US20240117244A1

    公开(公告)日:2024-04-11

    申请号:US18467380

    申请日:2023-09-14

    IPC分类号: C09K11/02 C09K11/06

    摘要: A method of treating a reaction mixture comprising light-emitting nanoparticles formed upon reaction of a silica-forming compound in the presence of a light-emitting material in a protic, water-miscible solvent. The method comprises forming a phase-separated system comprising: an aqueous phase comprising the protic, water miscible solvent, an organic phase comprising an organic solvent and a separating solvent; and separating the organic phase. The separating solvent is immiscible with water, miscible with the water-miscible protic solvent and miscible with the organic solvent.

    SEQUENCING METHOD
    10.
    发明申请

    公开(公告)号:US20220195513A1

    公开(公告)日:2022-06-23

    申请号:US17555151

    申请日:2021-12-17

    IPC分类号: C12Q1/6869 C12Q1/6809

    摘要: A method for determining the sequence of a polynucleotide is described. The method comprises detecting in a sequencing reaction the incorporation of a first, second, third, or fourth nucleotide using first and second excitation wavelengths and a photodetector having a detection window comprising a range of wavelengths.