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公开(公告)号：US20230086690A1
公开(公告)日：2023-03-23
申请号：US17940555
申请日：2022-09-08
Applicant: Sinfonia Technology Co., Ltd.
Inventor： Daichi Horii , Haruki Takeuchi
IPC: G01N1/14
Abstract: A cell collection method of collecting cells includes a culture medium movement process, a detachment liquid supply process, and a mixing process, wherein in the culture medium movement process, a used culture medium in a culture container is moved to a collection container, wherein in the detachment liquid supply process after the culture medium movement process, a detachment liquid for detaching the cells from an inner surface of the culture container is supplied to the culture container, and wherein in the mixing process after the detachment liquid supply process, the detachment liquid and the used culture medium are mixed with each other.
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公开(公告)号：US10361107B2
公开(公告)日：2019-07-23
申请号：US15738027
申请日：2016-06-08
Applicant: SINFONIA TECHNOLOGY CO., LTD.
Inventor： Haruki Takeuchi , Kazuhiro Tsuji , Minetaka Maeda
IPC: H01L21/677 , H01L21/673 , A61L2/20
Abstract: Reliability in the restriction of the movement of a container is improved.A content moving device 1 includes: a table portion 20 on which a container 50 is placed; and a restricting mechanism 30. A flange 55 of the container 50 is shaped to provide a recessed portion 56a. A restricting mechanism 30 includes: a collar portion 31; a first restricting portion 32 configured to be located in the recessed portion 56a; a second restricting portion 33a provided above the first restricting portion 32 and inward of the collar portion 31; and a second moving mechanism. The second moving mechanism is configured to move the second restricting portion 33a along an extending direction B between a first position, in which the second restricting portion 33a is opposed to the flange 55 of the container 50 in an up-down direction A, and a second position, in which the second restricting portion 33a is opposed to the recessed portion 56a in the up-down direction A without being opposed to the flange 55 in the up-down direction A.
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公开(公告)号：US20200208087A1
公开(公告)日：2020-07-02
申请号：US16723416
申请日：2019-12-20
Applicant: Sinfonia Technology Co., Ltd.
Inventor： Daichi Horii , Kazuhiro Tsuji , Yoshihiko Nishikawa , Haruki Takeuchi
Abstract: According to one embodiment of the present disclosure, a cell culture system includes: a cell culture container; a liquid storage part configured to store a liquid including a culture medium or a reagent to be supplied to the cell culture container; and a cell collection part configured to collect cells cultured in the cell culture container, wherein the cell culture container, the liquid storage part, and the cell collection part are connected by spatially closed-system lines at least during a period from feeding of the liquid to the cell culture container to removal of the cultured cells, and wherein the cell culture container is arranged in an incubator in a form of a multistage shelf including a liquid supply/discharge port.
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公开(公告)号：US20250051710A1
公开(公告)日：2025-02-13
申请号：US18930764
申请日：2024-10-29
Applicant: Sinfonia Technology Co., Ltd.
Inventor： Daichi Horii , Hiroyuki Uda , Hideki Maeda , Haruki Takeuchi
Abstract: A cell dispensing device capable of efficiently dispensing cells while reducing the risk of mixing of bacteria or the like is provided. The cell dispensing device includes a first tube configured to connect a container and a dispensing container to each other, a syringe pump attached to a second tube connected to a branch portion formed in the first tube, and a buffer tank provided between the syringe pump and the branch portion. The cell suspension stored in the container is temporarily stored in the buffer tank and is delivered to the dispensing container by the syringe pump.
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公开(公告)号：US11713440B2
公开(公告)日：2023-08-01
申请号：US16723416
申请日：2019-12-20
Applicant: Sinfonia Technology Co., Ltd.
Inventor： Daichi Horii , Kazuhiro Tsuji , Yoshihiko Nishikawa , Haruki Takeuchi
Abstract: According to one embodiment of the present disclosure, a cell culture system includes: a cell culture container; a liquid storage part configured to store a liquid including a culture medium or a reagent to be supplied to the cell culture container; and a cell collection part configured to collect cells cultured in the cell culture container, wherein the cell culture container, the liquid storage part, and the cell collection part are connected by spatially closed-system lines at least during a period from feeding of the liquid to the cell culture container to removal of the cultured cells, and wherein the cell culture container is arranged in an incubator in a form of a multistage shelf including a liquid supply/discharge port.
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Patent Agency Ranking
公开(公告)号：US20220243161A1
公开(公告)日：2022-08-04
申请号：US17586153
申请日：2022-01-27
Applicant: Sinfonia Technology Co., Ltd.
Inventor： Daichi Horii , Yasumichi Mieno , Haruki Takeuchi
Abstract: A cell culture device includes: a base; a rotary table attached to the base; a container holding member attached to the rotary table and configured to hold a cell culture container; and a drive mechanism configured to rotatably drive the rotary table around a first axis and the container holding member around a second axis, wherein a liquid port to which a liquid tube is connected is arranged on an outer peripheral surface of the cell culture container, wherein a first predetermined portion of the liquid tube is positioned on the container holding member by a first positioning member, wherein a second predetermined portion of the liquid tube is positioned on the rotary table by a second positioning member, and wherein the first positioning member is rotatably attached to the container holding member.
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公开(公告)号：US10781416B2
公开(公告)日：2020-09-22
申请号：US15766037
申请日：2016-10-04
Applicant: SINFONIA TECHNOLOGY CO., LTD.
Inventor： Haruki Takeuchi , Yoshimasa Suda
Abstract: A connection mechanism includes two connection parts. Each connection part includes a first flange including a through hole and an attaching portion by which the first flange is attached to a partial wall portion while the through hole faces an opening. The attaching portion includes: a vertical adjuster which adjusts the attaching position of the first flange so that a surface of the first flange, which is opposite to a surface of the first flange facing the partial wall portion, is vertical; and a rotation adjuster which adjusts the attaching position of the first flange about a horizontal axis passing the center of the through hole and extending in the thickness direction. The attaching position of a first flange can be individually adjusted.
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公开(公告)号：US20180362910A1
公开(公告)日：2018-12-20
申请号：US15736902
申请日：2016-06-15
Applicant: Entegris, Inc, , Tokyo Electron Limited , Sinfonia Technology Co., Ltd.
Inventor： Gregory Bores , Hisashi Gomi , Shigenori Ozaki , Akitake Tamura , Toshimitsu Fuji , Haruki Takeuchi
Abstract: A laboratory system has a plurality of bottom opening sample transport pods for carrying one or more cassettes with cell culture vessels therein may have an aseptic interior environment and may cooperate with cell culture growing/processing stations having load ports allowing transfer of the cassettes with cell culture vessels into the stations while maintaining the aseptic integrity of the interior of the pods and the cassette and cell culture vessels. Internal environmental monitoring and control, purging, and tracking as well as automated robotic operation may be provided.
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公开(公告)号：US20220364037A1
公开(公告)日：2022-11-17
申请号：US17620001
申请日：2020-06-15
Applicant: Sinfonia Technology Co., Ltd.
Inventor： Daichi Horii , Hiroyuki Uda , Hideki Maeda , Haruki Takeuchi
Abstract: The purpose of the present invention is to efficiently dispense cells while reducing the risk of incorporating bacteria, etc. A cell dispensing device 1 includes: a first tube 23a which connects a container 21 and a dispensing container 22; a syringe pump 24 attached to a second tube 23b connected to a branching portion 31 formed on the first tube 23a; and a buffer tank 25 provided between the syringe pump 24 and the branching portion 31. In addition, a cell suspension contained in the container 21 is temporarily stored in the buffer tank 25 by means of the syringe pump 24, and the cell suspension stored in the buffer tank 25 is delivered to the dispensing container 22.
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公开(公告)号：US20220356437A1
公开(公告)日：2022-11-10
申请号：US17619895
申请日：2020-06-15
Applicant: Sinfonia Technology Co., Ltd.
Inventor： Daichi Horii , Haruki Takeuchi , Yoshimasa Suda , Yuji Urabe
Abstract: There is provided a cell recovery method for recovering cells which are cultured in at least one container containing a liquid medium and adhere to an inner surface of the container, the method including performing: a medium discharge step of discharging the liquid medium from the container; a peeling liquid supply step of supplying a peeling liquid for peeling the cells from the inner surface of the container to the container; a peeling liquid discharge step of discharging the peeling liquid from the container before the cells are completely peeled from the inner surface of the container; a waiting step of waiting until the cells are peeled by action of a residual peeling liquid; and a recovery liquid supply step of supplying a recovery liquid for recovering the cells to the container.
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