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公开(公告)号：US20230002792A1
公开(公告)日：2023-01-05
申请号：US17755383
申请日：2020-10-27
申请人： Sumitomo Rubber Industries, Ltd. , TOHOKU UNIVERSITY , KANAZAWA UNIVERSITY , National University Corporation Saitama University
发明人： Haruhiko YAMAGUCHI , Yukino INOUE , Seiji TAKAHASI , Toru NAKAYAMA , Satoshi YAMASHITA , Yuzuru TOZAWA
IPC分类号： C12P5/00 , C12N9/10 , C07K14/415 , B60C1/00
摘要： Objects are to provide: a fusion protein capable of binding to lipid droplets while having an enzymatic activity to synthesize a hydrophobic compound; a method for producing a substance including accumulating a hydrophobic compound in lipid droplets using the fusion protein; a vector which can enhance production of a hydrophobic compound when it is introduced into cells using genetic recombination techniques; and a transgenic cell into which the vector or a gene coding for the fusion protein has been introduced. The present disclosure relates to a fusion protein having an amino acid sequence (first amino acid sequence) capable of binding to lipid droplets, and an amino acid sequence (second amino acid sequence) having an enzymatic activity to synthesize a hydrophobic compound, with the enzymatic activity of the second amino acid sequence being maintained.
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公开(公告)号：US20220002759A1
公开(公告)日：2022-01-06
申请号：US17412891
申请日：2021-08-26
发明人： Yuko SAKURAI , Haruhiko YAMAGUCHI , Yukino INOUE , Kazuhisa FUSHIHARA , Seiji TAKAHASHI , Satoshi YAMASHITA , Toru NAKAYAMA
摘要： The present invention aims to provide a method for producing a trans-polyisoprenoid which can increase trans rubber production. The present invention is directed to a method for producing a trans-polyisoprenoid in vitro, which involves the use of a gene coding for a trans-prenyltransferase (tPT) family protein and further involves the use of rubber particles bound to a protein encoded by the gene, or a method for producing a trans-polyisoprenoid, which includes introducing into a plant a vector including a promotor having a promoter activity that drives laticifer-specific gene expression and a gene coding for a tPT family protein linked to the promotor to express a protein encoded by the gene specifically in laticifers.
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公开(公告)号：US20240174989A1
公开(公告)日：2024-05-30
申请号：US18283332
申请日：2022-03-23
CPC分类号： C12N9/1085 , C07K1/36
摘要： The present disclosure aims to provide a method which, even when a protein complex is formed, can purify a protein derived from such a membrane protein complex by eluting the membrane protein complex maintaining its complex form. The present disclosure relates to a method for purifying a protein derived from a membrane protein complex, which includes: a protein binding step of binding to an antibody a membrane protein complex eluted from a membrane using a synthetic polymer; a protein eluting step of eluting the membrane protein complex bound to the antibody in the protein binding step at a pH of 6.0 or higher; and a synthetic polymer removing step of subjecting the membrane protein complex eluted in the protein eluting step to solvent extraction at a pH of 7.0 or lower to remove the synthetic polymer.
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公开(公告)号：US20230279370A1
公开(公告)日：2023-09-07
申请号：US18165026
申请日：2023-02-06
申请人： SUMITOMO RUBBER INDUSTRIES, LTD. , TOHOKU UNIVERSITY , KANAZAWA UNIVERSITY , NATIONAL UNIVERSITY CORPORATION SAITAMA UNIVERSITY
发明人： Haruhiko YAMAGUCHI , Yukino INOUE , Seiji TAKAHASHI , Toru NAKAYAMA , Satoshi YAMASHITA , Yuzuru TOZAWA
CPC分类号： C12N9/1085 , C12Y205/0102 , C12N15/8243 , C08L21/00
摘要： Provided are a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid. Included is a mutant cis-prenyltransferase (CET) family protein obtained by mutating the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles, and mutating the amino acid sequence of a C-terminal region of the cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles.
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5.发明申请公开(公告)号：US20170037156A1
公开(公告)日：2017-02-09
申请号：US15106560
申请日：2014-12-16
IPC分类号： C08C1/10
CPC分类号： C08C1/10 , C08C1/06 , C08J3/05 , C08J2307/02 , C08L7/02
摘要： The present invention provides an effective method for fractionating rubber particles in natural rubber latex by particle size, and effective methods for transporting or preserving rubber particles. The present invention relates to a method for fractionating rubber particles in natural rubber latex by particle size, the method including centrifuging natural rubber latex in at least four stages at forces of 800 to 3,000×g, 6,500 to 10,000×g, 17,000 to 22,000×g, and 40,000 to 60,000×g to fractionate rubber particles. The present invention also relates to methods for transporting or preserving rubber particles, the methods including the steps of adding a buffer solution and an antioxidant to natural rubber latex or rubber particles fractionated from natural rubber latex; and freezing a mixture prepared in the above step.
摘要翻译： 本发明提供了一种通过粒度对天然橡胶胶乳中的橡胶颗粒进行分级的有效方法，以及用于运输或保存橡胶颗粒的有效方法。 本发明涉及通过粒度分级天然橡胶胶乳中的橡胶粒子的方法，其特征在于，将800〜3000×g，6,500〜10,000×g，17,000〜22,000×g， g和40,000至60,000×g以分级橡胶颗粒。 本发明还涉及用于运输或保存橡胶颗粒的方法，所述方法包括以下步骤：向天然橡胶胶乳或天然橡胶胶乳分级的橡胶颗粒中加入缓冲溶液和抗氧化剂; 并冷冻在上述步骤中制备的混合物。
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公开(公告)号：US20230279371A1
公开(公告)日：2023-09-07
申请号：US18165191
申请日：2023-02-06
申请人： SUMITOMO RUBBER INDUSTRIES, LTD. , TOHOKU UNIVERSITY , KANAZAWA UNIVERSITY , NATIONAL UNIVERSITY CORPORATION SAITAMA UNIVERSITY
发明人： Haruhiko YAMAGUCHI , Yukino INOUE , Seiji TAKAHASHI , Toru NAKAYAMA , Satoshi YAMASHITA , Yuzuru TOZAWA
CPC分类号： C12N9/1085 , C12Y205/0102 , C12P5/007 , C12N15/8257 , B60C1/0041
摘要： Provided are a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid. Included is a mutant cis-prenyltransferase (CPT) family protein obtained by mutating the amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles.
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公开(公告)号：US20230167465A1
公开(公告)日：2023-06-01
申请号：US17799027
申请日：2021-02-22
发明人： Haruhiko YAMAGUCHI , Yukino INOUE , Kazuhisa FUSHIHARA , Seiji TAKAHASHI , Toru NAKAYAMA , Satoshi YAMASHITA
CPC分类号： C12P5/007 , C12N9/1085 , C12Y205/01028 , C12Y205/01089
摘要： The present disclosure provides a method for producing a polyisoprenoid, which makes it possible to synthesize in vitro a polyisoprenoid having an unprecedented structure, such as a 100% cis-polyisoprenoid or a polyisoprenoid containing an allylic diphosphate derivative as an initiating terminal. The present disclosure relates to a method for producing a polyisoprenoid in vitro, which employs a gene coding for a neryl diphosphate synthase and rubber particles bound to a protein encoded by the gene, or a method for producing a polyisoprenoid, which includes introducing into a plant a vector in which a gene coding for a neryl diphosphate synthase is linked to a promoter having a promoter activity that drives laticifer-specific gene expression to express a protein encoded by the gene specifically in laticifers.
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8.发明申请METHOD FOR PRODUCING POLYISOPRENOID, TRANSFORMED PLANT, METHOD FOR PRODUCING PNEUMATIC TIRE AND METHOD FOR PRODUCING RUBBER PRODUCT 审中-公开用于生产聚异戊二烯，变形植物的方法，用于生产气泡轮胎的方法和用于生产橡胶产品的方法公开(公告)号：US20170051313A1
公开(公告)日：2017-02-23
申请号：US15170584
申请日：2016-06-01
发明人： Yukino INOUE , Haruhiko YAMAGUCHI , Kazuhisa FUSHIHARA , Seiji TAKAHASHI , Satoshi YAMASHITA , Toru NAKAYAMA
CPC分类号： C12P5/007 , B29D30/0601 , B29D30/08 , B29K2607/00 , C07K14/415 , C12N9/1085 , C12N15/8243 , C12Y205/0102
摘要： Provided is a method for producing a polyisoprenoid, which can increase natural rubber production by enhancing the rubber synthesis activity of rubber particles. The present invention provides methods for producing a polyisoprenoid using a gene coding for a cis-prenyltransferase (CPT) family protein, a gene coding for a Nogo-B receptor (NgBR) family protein and a gene coding for a rubber elongation factor (REF) family protein, specifically a method for producing a polyisoprenoid in vitro using rubber particles bound to proteins coded for by these genes, and a method for producing a polyisoprenoid in vivo using a recombinant organism (plant) having these genes introduced therein.
摘要翻译： 提供了通过提高橡胶粒子的橡胶合成活性可以提高天然橡胶生产的聚异戊二烯的制造方法。 本发明提供使用编码顺式 - 异戊烯基转移酶（CPT）家族蛋白的基因，编码Nogo-B受体（NgBR）家族蛋白的基因和编码橡胶延伸因子（REF）的基因）来生产聚异戊二烯的方法， 家族蛋白质，特别是使用与这些基因编码的蛋白质结合的橡胶粒子在体外制备聚异戊二烯的方法，以及使用其中引入了这些基因的重组生物体（植物）在体内制备聚异戊二烯的方法。
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公开(公告)号：US20190323039A1
公开(公告)日：2019-10-24
申请号：US16502750
申请日：2019-07-03
发明人： Yukino INOUE , Haruhiko YAMAGUCHI , Kazuhisa FUSHIHARA , Seiji TAKAHASHI , Satoshi YAMASHITA , Toru NAKAYAMA
摘要： Provided is a method for producing a polyisoprenoid, which can increase natural rubber production by enhancing the rubber synthesis activity of rubber particles. The present invention provides methods for producing a polyisoprenoid using a gene coding for a cis-prenyltransferase (CPT) family protein, a gene coding for a Nogo-B receptor (NgBR) family protein and a gene coding for a rubber elongation factor (REF) family protein, specifically a method for producing a polyisoprenoid in vitro using rubber particles bound to proteins coded for by these genes, and a method for producing a polyisoprenoid in vivo using a recombinant organism (plant) having these genes introduced therein.
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公开(公告)号：US20180355000A1
公开(公告)日：2018-12-13
申请号：US15579499
申请日：2016-05-31
发明人： Haruhiko YAMAGUCHI , Yukino INOUE , Kazuhisa FUSHIHARA , Seiji TAKAHASHI , Satoshi YAMASHITA , Toru NAKAYAMA , Yuzuru TOZAWA
IPC分类号： C07K14/415 , C08G81/02 , B29D30/06 , C12N15/62 , B60C1/00
CPC分类号： C07K14/415 , B29D30/00 , B29D30/0601 , B29D2030/0055 , B60C1/00 , C08G81/02 , C08H1/00 , C08J2307/02 , C12N15/09 , C12N15/62
摘要： The present invention provides a method for producing rubber particles bound to a membrane-associated protein by cell-free protein synthesis. The present invention relates to a method for producing rubber particles bound to a membrane-associated protein, the method including the step of performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a membrane-associated protein to bind the membrane-associated protein to the rubber particles.
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