Abstract:
The disclosure relates generally to a cell culture apparatus and a cell culture method. One aspect of the disclosure provides a perfusion cell culture bag comprising one or more polymer films, having edges bonded together to form edges around an undivided interior compartment of the bag; first and second ports each formed in an exterior surface of the bag; first and second liquid-permeable tubes each extending into and in fluid communication with the interior compartment of the bag and operatively coupled to a respective port; and a third port formed in an exterior surface of the bag, the third port being in fluid communication with the interior compartment of the bag; wherein the first tube comprises a first inner support structure defining a central lumen of the tube and a first outer filter layer surrounding the first inner support structure.
Abstract:
A coated fabric includes a reinforcement, a first coating disposed on the reinforcement, and a second coating disposed on the first coating. The first coating includes perfluoropolymer. The second coating includes perfluoropolymer and a silicone polymer in an amount in a range of 2 wt % to 30 wt %.