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公开(公告)号：US12180444B2
公开(公告)日：2024-12-31
申请号：US17136400
申请日：2020-12-29
Inventor： Katie Campbell , Natalie Fekete , Rachel Z. Pytel
Abstract: This disclosure relates generally to containers (such as bags) having surfaces comprising one or more aptamer sequences. More particularly, the present disclosure relates to containers such as bags comprising a fluoropolymer attached to aptamer sequences having binding affinity for one or more biological agents, and to transductions methods using such containers. In one aspect, the disclosure provides a container (e.g., a bag) having an outer surface and an inner surface, the inner surface comprising a fluoropolymer; attached to the fluoropolymer, a plurality of functional groups; and attached to each of at least a portion of the functional groups, a first aptamer sequence having a binding affinity for a viral vector.
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Patent Agency Ranking
公开(公告)号：US20220204901A1
公开(公告)日：2022-06-30
申请号：US17564949
申请日：2021-12-29
Inventor： Katie Campbell , Natalie Fekete , Lei Zhang , Rachel Z. Pytel , Robert Gordon Pleydon
Abstract: The disclosure relates generally to a cell culture apparatus and a cell culture method. One aspect of the disclosure is an oxygen-permeable bag comprising one or more polymer films defining a boundary of an interior compartment of the bag, each of the one or more polymer films comprising an inner layer adjacent the interior compartment of the bag, the inner layer comprising a fluoropolymer, and adhered to the inner layer, an outer layer comprising polymer; a first port formed in an exterior surface of the bag and in fluid communication with the interior compartment; a second port formed in an exterior surface of the bag and in fluid communication with the interior compartment; and contained in the interior compartment, a plurality of microcarriers.
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公开(公告)号：US20240167019A1
公开(公告)日：2024-05-23
申请号：US18514893
申请日：2023-11-20
Inventor： Katie Campbell , Natalie Fekete , Jonathon Wheatley , Jessica Rodriguez , Maranda Gibb , Albert M. Liao , Thomas Caltagirone
IPC: C12N15/10 , C12M1/00 , C12M1/12 , C12N15/115 , C12N15/86
CPC classification number: C12N15/1082 , C12M23/14 , C12M25/14 , C12N15/1048 , C12N15/115 , C12N15/86 , C12N2310/16 , C12N2740/15043
Abstract: This disclosure relates generally to nucleic acid aptamers especially useful for cell transduction, as well as to containers (such as bags) having surfaces comprising one or such aptamers, and to transductions methods using such aptamers and containers. One embodiment of the disclosure provides A DNA aptamer comprising a plurality of nucleotides, the DNA aptamer having at least 80% sequence identity with the sequence of SEQ ID NO: 1 (AAACTGCAGCGATTCATTAGTACGGCCTTT).
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公开(公告)号：US20200209233A1
公开(公告)日：2020-07-02
申请号：US16730660
申请日：2019-12-30
Inventor： Katie Campbell , Natasha Anna Boghosian , Natalie Fekete , Rachel Z. Pytel
IPC: G01N33/543
Abstract: This disclosure relates to containers (e.g., in the form of bags) comprising a fluoropolymer and containing a degradable carrier comprising biological agent-capturing moieties, to cell isolation systems comprising such containers, and to methods for cultivating a biological agent using such containers. In one embodiment, the disclosure provides a container having an outer surface and an inner surface, the inner surface comprising a fluoropolymer, and contained in the container, a degradable carrier; wherein the carrier comprises a plurality of biological agent-capturing moieties.
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公开(公告)号：US20210198606A1
公开(公告)日：2021-07-01
申请号：US17136400
申请日：2020-12-29
Inventor： Katie Campbell , Natalie Fekete , Rachel Z. Pytel
Abstract: This disclosure relates generally to containers (such as bags) having surfaces comprising one or more aptamer sequences. More particularly, the present disclosure relates to containers such as bags comprising a fluoropolymer attached to aptamer sequences having binding affinity for one or more biological agents, and to transductions methods using such containers. In one aspect, the disclosure provides a container (e.g., a bag) having an outer surface and an inner surface, the inner surface comprising a fluoropolymer; attached to the fluoropolymer, a plurality of functional groups; and attached to each of at least a portion of the functional groups, a first aptamer sequence having a binding affinity for a viral vector.
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公开(公告)号：US20200207539A1
公开(公告)日：2020-07-02
申请号：US16730477
申请日：2019-12-30
Inventor： Natasha Anna Boghosian , Katie Campbell , Jian L. DIng , Natalie Fekete , Rachel Z. Pytel
Abstract: This disclosure relates to containers and substrates comprising a fluoropolymer and having a textured inner surface and to methods for cultivating and/or isolating a biological agent using such containers and substrates. In one embodiment, the disclosure provides a container (e.g., in the form of a bag) having an outer surface and a textured inner surface, the inner surface comprising a fluoropolymer, wherein the textured inner surface comprises one or more of (a) a plurality of functional groups attached to the fluoropolymer, and (b) a pattern of spaced features, the features having an average spacing within the range of 0.1 μm to 200 μm.
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