公开(公告)号：US08632966B2

公开(公告)日：2014-01-21

申请号：US13738620

申请日：2013-01-10

申请人： Queen's University at Kingston

发明人： R. Stephen Brown ,  Samir P. Tabash ,  Igor S. Kozin ,  Eric J. P. Marcotte ,  Arthur N. Ley ,  Kevin R. Hall ,  Moe Hussain ,  Peter V. Hodson ,  Raymond J. Bowers ,  Robin A. Wynne-Edwards ,  John G. St. Marseille

IPC分类号： C12Q1/00 ,  G01N21/64

CPC分类号： G01N33/56983 ,  B82Y15/00 ,  C12Q1/00 ,  C12Q1/04 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/56905 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/573 ,  G01N2021/6484 ,  Y10S435/808

摘要： This invention relates to a method and apparatus for detecting a biological molecule associated with enzyme activity in a sample. The invention is applicable to detecting a microorganism associated with an enzyme in a sample such as water, food, soil, or a biological sample. According to a preferred embodiment of the method of the invention, a sample containing an enzyme of interest or a microorganism associated with the enzyme is combined with a suitable substrate, and a fluorescent product of the enzyme-substrate reaction is selectively detected. The fluorescent product is detected with a partitioning element or optical probe/partitioning element of the invention. In one embodiment the partitioning element provides for partitioning of only the fluorescent product molecule into the probe. The invention also provides an automated system for monitoring for biological contamination of water or other samples.

摘要翻译： 本发明涉及一种用于检测与样品中酶活性相关的生物分子的方法和装置。 本发明适用于检测样品如水，食物，土壤或生物样品中与酶相关的微生物。 根据本发明方法的优选实施方案，将含有感兴趣的酶或与酶相关的微生物的样品与合适的底物组合，并选择性地检测酶 - 底物反应的荧光产物。 用本发明的分隔元件或光学探针/分隔元件检测荧光产品。 在一个实施方案中，分隔元件仅将荧光产物分子分配到探针中。 本发明还提供一种用于监测水或其它样品的生物污染的自动化系统。