公开(公告)号：US10240147B2

公开(公告)日：2019-03-26

申请号：US15106795

申请日：2014-12-11

Applicant: Philochem AG

Inventor： Willy Decurtins ,  Raphael Franzini ,  Dario Neri ,  Jörg Scheuermann ,  Moreno Wichert

IPC: C12N15/10 ,  C40B20/06 ,  C40B40/06 ,  C40B50/10 ,  G01N33/58 ,  C12Q1/6874 ,  C07H21/02 ,  C07H21/04

Abstract: This invention relates to the synthesis of nucleic acid-encoded chemical libraries using common adaptor sequences. Nucleic acid strands coupled to chemical moieties may be contacted with identifier oligonucleotides comprising coding sequences encoding the chemical moieties and an adaptor oligonucleotides, such that the adaptor oligonucleotide hybridizes to both the nucleic acid strands and the identifier oligonucleotides to allow ligation of the identifier oligonucleotides to the nucleic acid strands. The adaptor oligonucleotide is then removed. Nucleic acid-encoded chemical libraries, and methods of producing or screening such libraries are provided.

公开(公告)号：US20170009226A1

公开(公告)日：2017-01-12

申请号：US15106795

申请日：2014-12-11

Applicant: PHILOCHEM AG

Inventor： Willy Decurtins ,  Raphael Franzini ,  Dario Neri ,  Jörg Scheuermann ,  Moreno Wichert

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1068 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6874 ,  C40B20/06 ,  C40B40/06 ,  C40B50/10 ,  G01N33/58

Abstract: This invention relates to the synthesis of nucleic acid-encoded chemical libraries using common adaptor sequences. Nucleic acid strands coupled to chemical moieties may be contacted with identifier oligonucleotides comprising coding sequences encoding the chemical moieties and an adaptor oligonucleotides, such that the adaptor oligonucleotide hybridizes to both the nucleic acid strands and the identifier oligonucleotides to allow ligation of the identifier oligonucleotides to the nucleic acid strands. The adaptor oligonucleotide is then removed. Nucleic acid-encoded chemical libraries, and methods of producing or screening such libraries are provided.

Abstract translation: 本发明涉及使用公共衔接子序列合成核酸编码的化学文库。 与化学部分偶联的核酸链可以与包含编码化学部分的编码序列和衔接子寡核苷酸的标识符寡核苷酸接触，使得衔接寡核苷酸与核酸链和标识符寡核苷酸杂交，以使标识符寡核苷酸与 核酸链。 然后去除接头寡核苷酸。 提供了核酸编码的化学文库，以及生产或筛选这些文库的方法。