公开(公告)号：US5087563A

公开(公告)日：1992-02-11

申请号：US494004

申请日：1990-03-09

申请人： Phillip D. Beremand ,  Daniel J. Guerra

发明人： Phillip D. Beremand ,  Daniel J. Guerra

IPC分类号： C07K14/31 ,  C07K14/415 ,  C12N9/10

CPC分类号： C07K14/415 ,  C07K14/31 ,  C12N9/10 ,  C07K2319/00 ,  C07K2319/705

摘要： A gene encoding spinach acyl carrier protein-I-Gly.sup.76 (ACP-I) was fused to a gene encoding the F.sub.c -binding portion of staphylococcal protein A. The gene fusion can be expressed in E. coli thereby resulting in the production of an appropriately sized protein possessing ACP activity in acyl ACP synthetase reactions, plant MCT reactions, and plant fatty acid synthesis. This gene fusion, under the control of the .lambda.P.sub.R promoter, expresses high levels of a 42 kDa fusion protein in E. coli. This protein functions as a affinity moiety in purification methods for the isolation of enzymes involved in fatty acid synthetase, acyl ACP synthetase and malonyl-CoA:ACP tranacylase reactions.

摘要翻译： 编码菠菜酰基载体蛋白-I-Gly76（ACP-1）的基因与编码葡萄球菌蛋白A的Fc结合部分的基因融合。基因融合可以在大肠杆菌中表达，从而产生适当的 在酰基ACP合成酶反应，植物MCT反应和植物脂肪酸合成中具有ACP活性的大小蛋白质。 在λPR启动子控制下的该基因融合在大肠杆菌中表达高水平的42kDa融合蛋白。 该蛋白质在用于分离涉及脂肪酸合成酶，酰基ACP合成酶和丙二酰辅酶A：ACP葡糖酰胺反应的酶的纯化方法中起到亲和性部分的作用。

公开(公告)号：US5959175A

公开(公告)日：1999-09-28

申请号：US831570

申请日：1997-04-09

申请人： Terry L. Thomas ,  Andrew N. Nunberg ,  Phillip D. Beremand

发明人： Terry L. Thomas ,  Andrew N. Nunberg ,  Phillip D. Beremand

IPC分类号： A01H5/00 ,  C07K14/415 ,  C12N1/21 ,  C12N5/10 ,  C12N9/02 ,  C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  A01H5/10 ,  C12P7/64

CPC分类号： C12N9/0083 ,  C07K14/415 ,  C12N15/8234 ,  C12N15/8247

摘要： The present invention is directed to 5' regulatory regions of a sunflower albumin gene. The 5' regulatory regions, when operably linked to either the coding sequence of a heterologous gene or a sequence complementary to a native plant gene direct expression of the coding sequence or complementary sequence in a plant seed. The regulatory regions are useful in expression cassettes and expression vectors for the transformation of plants.Also provided are methods of modulating the levels of a heterologous gene or native plant gene such as a fatty acid synthesis or lipid metabolism gene by transforming a plant with the subject expression cassettes and expression vectors.

摘要翻译： 本发明涉及向日葵白蛋白基因的5'调节区。 当与异源基因的编码序列或与天然植物基因互补的序列可操作地连接时，5'调节区域在植物种子中直接表达编码序列或互补序列。 调节区可用于植物转化的表达盒和表达载体。 还提供了通过用受试者表达盒和表达载体转化植物来调节异源基因或天然植物基因如脂肪酸合成或脂质代谢基因的水平的方法。