公开(公告)号：US20160298159A1

公开(公告)日：2016-10-13

申请号：US14911876

申请日：2013-09-29

申请人： PEPSICO INC.

发明人： Junhua TAO ,  Guoqing LI ,  Xiaoliang LIANG ,  Thomas LEE ,  Gregory YEP ,  Andrew TAO

IPC分类号： C12P19/56 ,  C12P19/18

CPC分类号： C12P19/56 ,  A23L27/36 ,  A23V2002/00 ,  C12N9/1051 ,  C12P19/18 ,  C12Y204/01017

摘要： Provided is a method for preparing rebaudioside M by using an enzyme method. In the method, rebaudioside A or rebaudioside D is used as a substrate; and in the existence of a glucosyl donor, rebaudioside M is generated by means of reaction of the substrate under the catalysis of UDP-glucosyl transferase and/or recombinant cells containing the UDP-glucosyl transferase.

摘要翻译： 提供了使用酶法制备新蛇菊苷M的方法。 在该方法中，使用新蛇菊苷A或莱鲍迪甙D作为底物; 并且在葡糖基供体的存在下，通过在UDP-葡糖基转移酶的催化下和/或含有UDP-葡糖基转移酶的重组细胞的底物反应产生新蛇菊苷M。

公开(公告)号：US20220220525A1

公开(公告)日：2022-07-14

申请号：US17657307

申请日：2022-03-30

申请人： PepsiCo, Inc.

发明人： Alex TAO ,  Guoqing LI ,  Wenxia WANG ,  Leilei ZHENG ,  Chunlei ZHU ,  Xiaoliang LIANG ,  Kuikiu CHAN

IPC分类号： C12P19/56

摘要： Provided is a method for preparing Rehaudioside C using an enzymatic method, comprising using rubusoside or dulcoside A as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of UDP-glycosyltransferase-containing recombinant cell and/or UDP-glycosyltransferase prepared therefrom to generate Rebaudioside C.

公开(公告)号：US20210214761A1

公开(公告)日：2021-07-15

申请号：US16343335

申请日：2016-10-21

申请人： PepsiCo, Inc.

发明人： Alex TAO ,  Guoqing LI ,  Wenxia WANG ,  Leilei ZHENG ,  Chunlei ZHU ,  Xiaoliang LIANG ,  Kuikiu CHAN

IPC分类号： C12P19/56 ,  C12N9/10

摘要： Provided is a method for preparing rebaudioside N using an enzymatic method, comprising using rebaudioside A or rebaudioside J as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyl-transferase and/or a UDP-glycosyltransferase-containing recombinant cell to generate rebaudioside N.

公开(公告)号：US20190233866A1

公开(公告)日：2019-08-01

申请号：US16380678

申请日：2019-04-10

申请人： PepsiCo, Inc.

发明人： Junhua TAO ,  Guoqing LI ,  Xiaoliang LIANG ,  Andrew TAO

IPC分类号： C12P19/56 ,  C12P19/18 ,  C12N9/10 ,  A23L27/30

CPC分类号： C12P19/56 ,  A23L27/36 ,  A23V2002/00 ,  C12N9/1051 ,  C12P19/18 ,  C12Y204/01017

摘要： Provided is a method for preparing rebaudioside M by using an enzyme method. In the method, rebaudioside A or rebaudioside D is used as a substrate; and in the existence of a glucosyl donor, rebaudioside M is generated by means of reaction of the substrate under the catalysis of UDP-glucosyl transferase and/or recombinant cells containing the UDP-glucosyl transferase.

公开(公告)号：US20240287567A1

公开(公告)日：2024-08-29

申请号：US18600332

申请日：2024-03-08

申请人： PepsiCo, Inc.

发明人： Alex TAO ,  Guoqing LI ,  Wenxia WANG ,  Leilei ZHENG ,  Chunlei ZHU ,  Xiaoliang LIANG ,  Kuikiu CHAN

IPC分类号： C12P19/56 ,  C12N9/10

CPC分类号： C12P19/56 ,  C12N9/1051 ,  C12Y204/01017

摘要： Provided is a method for preparing Rebaudioside J using an enzymatic method, comprising using rebaudioside A as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyltransferase-containing recombinant cell and/or UDP-glycosyltransferase prepared therefrom to generate Rebaudioside J.

公开(公告)号：US20230022453A1

公开(公告)日：2023-01-26

申请号：US17805626

申请日：2022-06-06

申请人： PepsiCo, Inc.

发明人： Alex TAO ,  Guoqing LI ,  Wenxia WANG ,  Leilei ZHENG ,  Chunlei ZHU ,  Xiaoliang LIANG ,  Kuikiu CHAN

IPC分类号： C12P19/56 ,  C12N9/10

摘要： Provided is a method for preparing Rebaudioside J using an enzymatic method, comprising using rebaudioside A as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyltransferase-containing recombinant cell and/or UDP-glycosyltransferase prepared therefrom to generate Rebaudioside J.

公开(公告)号：US20220275415A1

公开(公告)日：2022-09-01

申请号：US17663631

申请日：2022-05-16

申请人： PepsiCo, Inc.

发明人： Alex TAO ,  Guoqing LI ,  Wenxia WANG ,  Leilei ZHENG ,  Chunlei ZHU ,  Xiaoliang LIANG ,  Kuikiu CHAN

IPC分类号： C12P19/56 ,  C12N9/10

摘要： Provided is a method for preparing rebaudioside N using an enzymatic method, comprising using rebaudioside A or rebaudioside J as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyl-transferase and/or a UDP-glycosyltransferase-containing recombinant cell to generate rebaudioside N.

公开(公告)号：US20190338332A1

公开(公告)日：2019-11-07

申请号：US16343340

申请日：2016-10-21

申请人： PepsiCo, Inc.

发明人： Alex TAO ,  Guoqing LI ,  Wenxia WANG ,  Leilei ZHENG ,  Chunlei ZHU ,  Xiaoliang LIANG ,  Kuikiu CHAN

IPC分类号： C12P19/56 ,  C12N9/10

摘要： Provided is a method for preparing Rebaudioside J using an enzymatic method, comprising using rebaudioside A as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyltransferase-containing recombinant cell and/or UDP-glycosyltransferase prepared therefrom to generate Rebaudioside J.

公开(公告)号：US20170211113A1

公开(公告)日：2017-07-27

申请号：US15114250

申请日：2014-01-28

申请人： PepsiCo, Inc.

发明人： Junhua TAO ,  Guoqing LI ,  Xiaoliang LIANG ,  Thomas LEE ,  Gregory YEP ,  Maoqi HOU ,  Andrew TAO

IPC分类号： C12P19/56

CPC分类号： C12P19/56 ,  C12Y204/01013 ,  C12Y204/01051

摘要： Provided is a method for preparing Rebaudioside M by using an enzyme method. In the method, Rebaudioside A or Rebaudioside D is used as a substrate, and in the presence of sucrose and UDP, Rebaudioside M is generated by means of reaction of the substrate under the catalysis of a mixture of UDP-glucosyl transferase and sucrose synthetase, or recombinant cells containing the UDP-glucosyl transferase and sucrose synthetase. The reaction is carried out in an aqueous-phase system at a temperature of 20 to 60° C. and pH 5.0 to 9.0. The reaction system further contains dimethyl sulfoxide at a concentration of 3% to 5% according to the ratio by volume for facilitating solubilization of the substrate.