公开(公告)号：US20210214761A1

公开(公告)日：2021-07-15

申请号：US16343335

申请日：2016-10-21

申请人： PepsiCo, Inc.

发明人： Alex TAO ,  Guoqing LI ,  Wenxia WANG ,  Leilei ZHENG ,  Chunlei ZHU ,  Xiaoliang LIANG ,  Kuikiu CHAN

IPC分类号： C12P19/56 ,  C12N9/10

摘要： Provided is a method for preparing rebaudioside N using an enzymatic method, comprising using rebaudioside A or rebaudioside J as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyl-transferase and/or a UDP-glycosyltransferase-containing recombinant cell to generate rebaudioside N.

公开(公告)号：US20220220525A1

公开(公告)日：2022-07-14

申请号：US17657307

申请日：2022-03-30

申请人： PepsiCo, Inc.

发明人： Alex TAO ,  Guoqing LI ,  Wenxia WANG ,  Leilei ZHENG ,  Chunlei ZHU ,  Xiaoliang LIANG ,  Kuikiu CHAN

IPC分类号： C12P19/56

摘要： Provided is a method for preparing Rehaudioside C using an enzymatic method, comprising using rubusoside or dulcoside A as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of UDP-glycosyltransferase-containing recombinant cell and/or UDP-glycosyltransferase prepared therefrom to generate Rebaudioside C.

公开(公告)号：US20240287567A1

公开(公告)日：2024-08-29

申请号：US18600332

申请日：2024-03-08

申请人： PepsiCo, Inc.

发明人： Alex TAO ,  Guoqing LI ,  Wenxia WANG ,  Leilei ZHENG ,  Chunlei ZHU ,  Xiaoliang LIANG ,  Kuikiu CHAN

IPC分类号： C12P19/56 ,  C12N9/10

CPC分类号： C12P19/56 ,  C12N9/1051 ,  C12Y204/01017

摘要： Provided is a method for preparing Rebaudioside J using an enzymatic method, comprising using rebaudioside A as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyltransferase-containing recombinant cell and/or UDP-glycosyltransferase prepared therefrom to generate Rebaudioside J.

公开(公告)号：US20230022453A1

公开(公告)日：2023-01-26

申请号：US17805626

申请日：2022-06-06

申请人： PepsiCo, Inc.

发明人： Alex TAO ,  Guoqing LI ,  Wenxia WANG ,  Leilei ZHENG ,  Chunlei ZHU ,  Xiaoliang LIANG ,  Kuikiu CHAN

IPC分类号： C12P19/56 ,  C12N9/10

摘要： Provided is a method for preparing Rebaudioside J using an enzymatic method, comprising using rebaudioside A as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyltransferase-containing recombinant cell and/or UDP-glycosyltransferase prepared therefrom to generate Rebaudioside J.

公开(公告)号：US20220275415A1

公开(公告)日：2022-09-01

申请号：US17663631

申请日：2022-05-16

申请人： PepsiCo, Inc.

发明人： Alex TAO ,  Guoqing LI ,  Wenxia WANG ,  Leilei ZHENG ,  Chunlei ZHU ,  Xiaoliang LIANG ,  Kuikiu CHAN

IPC分类号： C12P19/56 ,  C12N9/10

摘要： Provided is a method for preparing rebaudioside N using an enzymatic method, comprising using rebaudioside A or rebaudioside J as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyl-transferase and/or a UDP-glycosyltransferase-containing recombinant cell to generate rebaudioside N.

公开(公告)号：US20190338332A1

公开(公告)日：2019-11-07

申请号：US16343340

申请日：2016-10-21

申请人： PepsiCo, Inc.

发明人： Alex TAO ,  Guoqing LI ,  Wenxia WANG ,  Leilei ZHENG ,  Chunlei ZHU ,  Xiaoliang LIANG ,  Kuikiu CHAN

IPC分类号： C12P19/56 ,  C12N9/10

摘要： Provided is a method for preparing Rebaudioside J using an enzymatic method, comprising using rebaudioside A as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyltransferase-containing recombinant cell and/or UDP-glycosyltransferase prepared therefrom to generate Rebaudioside J.