NANO-PROBE FOR MEASURING pH IN SINGLE CELLS, AND METHOD AND APPARATUS FOR MEASURING pH IN SINGLE CELLS USING THE SAME

    公开(公告)号:US20220244278A1

    公开(公告)日:2022-08-04

    申请号:US17588571

    申请日:2022-01-31

    Abstract: Provided is a method and apparatus for measuring pH in single cells, and a method of manufacturing a nanoprobe therefor. The apparatus for measuring pH in a single cell comprises: a nanoprobe formed by labeling a pH-responsive fluorescent material to a nanowire grown on a tapered tip of an optical fiber; a manipulator capable of regulating a three-dimensional movement of the nanoprobe to insert the nanoprobe into a single living cell; a light source for applying light to the optical fiber; an optical coupler for connecting the optical fiber with another optical fiber to transmit the light incident through the optical fiber to the nanoprobe and to transmit a fluorescence signal obtained from the nanoprobe through the another optical fiber; and a spectrometer for obtaining a pH value by receiving the fluorescence signal through the another optical fiber and analyzing spectral data from the fluorescence signal.

    METHOD FOR FABRICATING MICRO- OR NANOWIRE AT PREDETERMINED POSITION OF OBJECT USING MICRO- OR NANOPIPETTE

    公开(公告)号:US20220242726A1

    公开(公告)日:2022-08-04

    申请号:US17587922

    申请日:2022-01-28

    Abstract: Provided is a method of fabricating a micro/nanowire having a nanometer- to micrometer-sized diameter at predetermined positions on an object. The method comprises: preparing a micro/nanopipette having a tip with an inner diameter (dpt) which is substantially the same as the diameter of the micro/nanowire to be fabricated; filling the micro/nanopipette with a solution containing a micro/nanowire-forming material; bringing the solution into contact with the object through the tip of the micro/nanopipette; and pulling the micro/nanopipette apart from the object at a pulling speed lower than or equal to a predetermined critical pulling speed (vc) to fabricate a micro/nanowire having substantially the same diameter as the inner diameter of the micro/nanopipette tip (dpt). The critical pulling speed (vc) is defined by a maximum limit of the pulling speed at which the micro/nanowire to be fabricated has the same diameter as the inner diameter of the micro/nanopipette tip (dpt).

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