公开(公告)号：US20220372437A1

公开(公告)日：2022-11-24

申请号：US17765637

申请日：2020-09-09

申请人： OSAKA UNIVERSITY

发明人： Takaaki SAMURA ,  Shigeru MIYAGAWA ,  Yoshiki SAWA ,  Junya YOKOYAMA

IPC分类号： C12N5/00 ,  C07K14/75

摘要： A method for producing a fibrin sheet containing at least one selected from the group consisting of cells and drugs in a fibrin gel, the method comprising: a step 1 of applying a fibrinogen solution containing at least one selected from the group consisting of cells and drugs and fibrinogen dropwise onto a surface of a substrate made of a gelatin hydrogel; a step 2 of adding thrombin to the fibrinogen solution on the surface of the substrate; a step 3 of placing a support film on and in contact with a top surface of the fibrinogen solution to which the thrombin has been added; a step 4 of forming a fibrin sheet containing the at least one selected from the group consisting of cells and drugs in a fibrin gel between the substrate and the support film by a reaction between the fibrinogen and the thrombin; and a step 5 of melting the substrate at a temperature not lower than a melting temperature of the gelatin hydrogel to separate, from the substrate, the fibrin sheet supported by the support film.