公开(公告)号：US20220412982A1

公开(公告)日：2022-12-29

申请号：US17776920

申请日：2020-11-16

申请人： NATIONAL UNIVERSITY OF SINGAPORE

发明人： Huilin SHAO ,  Zhigang WANG ,  Xuecheng SUN

IPC分类号： G01N33/574 ,  G01N33/543 ,  G01N33/68 ,  G01N27/74

摘要： Devices and methods for analysing extracellular vesicle glycans are described. According to an embodiment, a microfluidic device comprises an inlet portion configured to receive a fluid sample; a mixing portion fluidically coupled to the inlet portion and configured to facilitate mixing between the fluid sample and magnetic nanoparticles functionalized to bind with extracellular vesicles and aggregate to vesicle glycans in the fluid sample; a magnetic separation portion fluidically coupled to the mixing portion and configured to separate clusters of magnetic nanoparticles from the fluid sample; and a magnetic sensor configured to measure magnetic properties of the fluid sample after it has passed through the magnetic separation portion. The magnetic nanoparticles may configured to aggregate in the presence of respective lectins when bound with extracellular vesicles carrying target glycans. In a specific embodiment, the magnetic particles comprise a magnetic polycore coated with polydopamine.