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1.发明公开公开(公告)号：US20240301353A1
公开(公告)日：2024-09-12
申请号：US18575460
申请日：2022-06-30
Inventor： Naoya TAKAYAMA , Koji ETO , Sou NAKAMURA , Sudip Kumar PAUL
IPC: C12N5/0786 , C12N5/10
CPC classification number: C12N5/0645 , C12N5/10 , C12N2501/125 , C12N2501/145 , C12N2501/22 , C12N2501/2303 , C12N2510/04
Abstract: The present invention relates to a method for improving proliferative property of a CMP or a myeloid progenitor cell, including a step of forcibly expressing a MYC family gene and BMI1 gene in any cell in the process of differentiation from a hematopoietic progenitor cell into a myeloid progenitor cell, wherein the myeloid progenitor cell is a progenitor cell of macrophage, dendritic cell, granulocyte, erythroblast, or erythrocyte.
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2.发明申请公开(公告)号：US20220010283A1
公开(公告)日：2022-01-13
申请号：US17289970
申请日：2019-10-30
Applicant: Kyoto University
Inventor： Koji ETO , Akinori YUZURIHA
IPC: C12N5/074 , G01N33/50 , C12N5/0789
Abstract: The present invention provides a method for producing a pluripotent stem cell released from resistance to differentiation into mesendodermal lineage, including subjecting a pluripotent stem cell to a treatment for activating an integrin β1-β-catenin signal transduction pathway (excluding contacting the pluripotent stem cell with laminin 421 or a fragment thereof and/or laminin 121 or a fragment thereof), and the like.
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公开(公告)号：US20230147253A1
公开(公告)日：2023-05-11
申请号：US17904441
申请日：2021-02-18
Applicant: KYOTO UNIVERSITY
Inventor： Koji ETO , Sou NAKAMURA , Shinya SAKUMA , Hiroki KUMON , Fumihito ARAI , Yusuke KASAI
CPC classification number: C12N5/0644 , B01L3/502761 , B01L2200/0647 , B01L2300/0816
Abstract: The present invention provides a method for producing a platelet comprising: (a) a step of culturing a megakaryocyte for at least 6 days in a platelet production medium in which a turbulent flow is generated; and (b) a step of injecting the medium comprising the megakaryocyte that has undergone step (a) into a predetermined platelet production device to expose the megakaryocyte to a laminar flow.
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公开(公告)号：US20160002599A1
公开(公告)日：2016-01-07
申请号：US14763746
申请日：2014-02-10
Applicant: KYOTO UNIVERSITY
Inventor： Koji ETO
IPC: C12N5/078
CPC classification number: C12N5/0644 , A61K35/19 , A61K35/28 , C12N2501/125 , C12N2501/145 , C12N2501/48 , C12N2501/734 , C12N2502/13 , C12N2506/11
Abstract: An object of the present invention is to provide a method of efficiently producing a maturated megakaryocytic cell line from hematopoietic progenitor cells. The present invention provides a method for producing megakaryocytes from hematopoietic progenitor cells, comprising (i) forcibly expressing an apoptosis suppression gene and an oncogene in hematopoietic progenitor cells and culturing the cells, and (ii) arresting forced expression of the apoptosis suppression gene and the oncogene and culturing the hematopoietic progenitor cells.
Abstract translation: 本发明的目的是提供从造血祖细胞有效地产生成熟的巨核细胞系的方法。 本发明提供了一种从造血祖细胞产生巨核细胞的方法，其特征在于包括：（i）在造血祖细胞中强制表达凋亡抑制基因和致癌基因并培养细胞，以及（ii）阻止凋亡抑制基因的强制表达 致癌基因并培养造血祖细胞。
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公开(公告)号：US20240191195A1
公开(公告)日：2024-06-13
申请号：US18585322
申请日：2024-02-23
Applicant: KYOTO UNIVERSITY , MEGAKARYON CORPORATION
Inventor： Koji ETO , Sou NAKAMURA , Yukitaka ITO
CPC classification number: C12N5/0644 , C12N15/85 , C12N2501/105 , C12N2506/02
Abstract: Healthy functional platelets are mass produced. A method for producing platelets, comprising: (1) a culture step of culturing megakaryocytes in a platelet producing medium in the presence of mechanical stress and platelet production promoting factors including MIF, NRDc, IGFBP2, TSP-1, PAI-1, and CCL5, and (2) a harvest step of harvesting the platelets obtained by the culture step; wherein the culture step comprises: (a) a step of promoting a release of the platelet production promoting factors from megakaryocytes by mechanical stress; and/or (b) a step of externally adding platelet production promoting factors including MIF, NRDc, and IGFBP2.
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Patent Agency Ranking
公开(公告)号：US20220017866A1
公开(公告)日：2022-01-20
申请号：US17395552
申请日：2021-08-06
Applicant: KYOTO UNIVERSITY
Inventor： Koji ETO
Abstract: An object of the present invention is to provide a method of efficiently producing a maturated megakaryocytic cell line from hematopoietic progenitor cells. The present invention provides a method for producing megakaryocytes from hematopoietic progenitor cells, comprising (i) forcibly expressing an apoptosis suppression gene and an oncogene in hematopoietic progenitor cells and culturing the cells, and (ii) arresting forced expression of the apoptosis suppression gene and the oncogene and culturing the hematopoietic progenitor cells.
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公开(公告)号：US20230062476A1
公开(公告)日：2023-03-02
申请号：US17797551
申请日：2021-03-01
Applicant: OTSUKA PHARMACEUTICAL CO., LTD. , KYOTO UNIVERSITY
Inventor： Yasuo HARADA , Akitsu HOTTA , Huaigeng XU , Naoshi SUGIMOTO , Koji ETO
Abstract: A production method for a genetically modified megakaryocyte, the method including a step of introducing a CRISPR-associated (Cas) family protein and guide RNA (gRNA) into a megakaryocyte to modify a subject gene, in which the Cas family protein and the gRNA to be introduced into the megakaryocyte in the step form a complex beforehand. This production method is useful as a technique for producing a genetically modified megakaryocyte and a modified platelet.
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公开(公告)号：US20210130781A1
公开(公告)日：2021-05-06
申请号：US16628451
申请日：2018-07-05
Inventor： Koji ETO , Sou NAKAMURA , Yukitaka ITO , Tomohiro SHIGEMORI , Yoshikazu KATO
Abstract: Methods of production and apparatuses for production for stably producing healthy and functional platelets, and methods for determining operating conditions of an apparatus for producing platelets are provided. The present invention relates to methods of production comprising a step of culturing megakaryocytes in a platelet production medium C, wherein the step of culturing comprises a step of stirring a medium C in containers 1, 4 using stirring mechanisms 2, 5. In one method of production, the step of stirring comprises reciprocating stirring blades 21, 24 in the stirring mechanisms 2, 24 such that any of predetermined turbulent energy, shearing stresses, or Kolmogorov scale is produced, in the medium C. In another method of production, the step of stirring comprises pivoting a stirring blade 51 in the stirring mechanism 5 wherein a plurality of stationary members 53 is placed therearound in a bottom region 4e of the container 4 such that the step of stirring produces turbulence in the medium C. The present invention also relates to apparatuses for production for carrying out the method of production. The present invention also relates to a method for determining operating conditions of an apparatus for producing platelets.
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公开(公告)号：US20190071636A1
公开(公告)日：2019-03-07
申请号：US16083503
申请日：2017-03-22
Applicant: Kyoto University , Megakaryon Corporation
Inventor： Koji ETO , Hiroshi ENDO , Yukitaka ITO , Junichi FUKUNAGA
IPC: C12N5/0735 , A61K35/19 , A61P7/08 , C12N5/074 , A61K38/18
Abstract: Provided is a method for inducing mesoderm, comprising a step of bringing pluripotent stem cells into contact with bone morphogenetic protein 4 (BMP4) or CHIR for at least 3 days.
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公开(公告)号：US20160168540A1
公开(公告)日：2016-06-16
申请号：US14895778
申请日：2014-06-06
Applicant: KYOTO UNIVERSITY , KAKEN PHARMACEUTICAL CO., LTD.
Inventor： Shinji HIRATA , Takahiko MURATA , Koji ETO
IPC: C12N5/078 , C07D401/06
CPC classification number: C12N5/0644 , A61K31/443 , A61K31/4436 , A61K31/4439 , A61K31/444 , A61K35/19 , C07D401/06 , C12N2501/734
Abstract: Provided is a composition for maintaining a function of platelets, having as an active ingredient a pyridone derivative represented by the following general formula (I), or a salt thereof (in the formula, ring A, R1, R2, R3, and R4 represent the definitions given in the description).
Abstract translation: 本发明提供一种维持血小板功能的组合物，其具有作为活性成分的由以下通式（I）表示的吡啶酮衍生物或其盐（式中，环A，R 1，R 2，R 3和R 4表示 描述中给出的定义）。
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