公开(公告)号：US4834976A

公开(公告)日：1989-05-30

申请号：US946554

申请日：1986-12-24

申请人： Mae J. Rosok ,  Mark E. Lostrom

发明人： Mae J. Rosok ,  Mark E. Lostrom

IPC分类号： A61K38/00 ,  C07K16/12 ,  G01N33/569

CPC分类号： C07K16/1214 ,  G01N33/56911 ,  A61K38/00 ,  Y10S435/804 ,  Y10S435/875 ,  Y10S436/811

摘要： Cell lines have been produced that secrete monoclonal antibodies capable of binding to the flagellar proteins of selected Pseudomonas aeruginosa strains. Some of these antibodies have been found to be protective against lethal challenges of P. aeruginosa. Pharmaceutical compositions containing these antibodies, which can be in combination with other monoclonal antibodies, blood plasma fractions and antimicrobial agents, and the prophylactic and therapeutic use of such compositions in the management of infections, are included.Prior to filing this application, the continuous transformed cell lines PaF4 IVE8, FA6 IIG5, 20H11, and 21B8, described herein, were deposited in the America Type Culture Collection and given the designations HB9129, HB9130, CRL 9300, and CRL 9301, respectively.

摘要翻译： 已经产生分泌能够结合选定的铜绿假单胞菌菌株的鞭毛蛋白的单克隆抗体的细胞系。 已经发现这些抗体中的一些是针对铜绿假单胞菌的致死性挑战的保护物。 包含可与其它单克隆抗体组合的这些抗体，血浆级分和抗微生物剂的药物组合物，以及这些组合物在感染管理中的预防和治疗用途。 在提交本申请之前，将本文所述的连续转化细胞系PaF4 IVE8，FA6 IIG5,20H11和21B8保藏在美国典型培养物保藏中，并分别给予HB9129，HB9130，CRL9300和CRL 9301。

公开(公告)号：US5716829A

公开(公告)日：1998-02-10

申请号：US452845

申请日：1995-05-30

申请人： Mae Joanne Rosok ,  Mark E. Lostrom ,  Richard P. Darveau

发明人： Mae Joanne Rosok ,  Mark E. Lostrom ,  Richard P. Darveau

IPC分类号： G01N33/569

CPC分类号： G01N33/56911 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/975 ,  Y10S436/825 ,  Y10T436/25125

摘要： Methods and reagents are provided for a novel immunodiagnostic test for the presence of P. aeruginosa in a biological sample. Monoclonal antibodies are employed which bind to an outer membrane protein antigen, which may be exposed by a solubilizing reagent. The antibody binds substantially all strains of P. aeruginosa. No cross-reactivity with other species of Pseudomonas or with other clinically significant gram-negative or gram-positive species is observed. Conjugating the monoclonal antibody of the invention with a fluorescent label provides for a rapid and sensitive direct immunofluorescent test for P. aeruginosa.

摘要翻译： 提供了用于生物样品中铜绿假单胞菌存在的新型免疫诊断试验的方法和试剂。 使用结合外膜蛋白抗原的单克隆抗体，其可以通过增溶试剂暴露。 抗体基本上结合所有铜绿假单胞菌菌株。 没有观察到与其他种类的假单胞菌或其他临床重要的革兰氏阴性或革兰氏阳性物种的交叉反应。 将本发明的单克隆抗体与荧光标记物缀合提供对铜绿假单胞菌的快速和敏感的直接免疫荧光试验。

公开(公告)号：US4464465A

公开(公告)日：1984-08-07

申请号：US365218

申请日：1982-04-05

申请人： Mark E. Lostrom

发明人： Mark E. Lostrom

IPC分类号： C12N15/02 ,  C07K16/00 ,  C12N5/10 ,  C12N15/09 ,  C12P21/08 ,  C12R1/91 ,  C12P21/00 ,  C12N5/00 ,  C12N15/00

CPC分类号： C07K16/00 ,  Y10S435/948

摘要： Novel method, cells and compositions are provided involving transforming B-lymphocytes to provide immortalization for continuous production of monoclonal antibodies to a predetermined ligand. T-cell free B-lymphocytes are combined with an Epstein-Barr virus transformed cell sensitive to a cytotoxic agent which does not significantly affect the B-lymphocytes under conditions where the sensitive EBV transformed cell acting as the transfer agent is killed and efficiently transforms the B-lymphocyte recipient cells with EBV. The EBV transformed B-lymphocyte cells are amplified and cloned, the desired clones isolated in accordance with conventional techniques and then used for production of monoclonal antibodies.

摘要翻译： 提供新的方法，细胞和组合物涉及转化B淋巴细胞以提供用于连续制备预定配体的单克隆抗体的永生化。 将不含T细胞的B淋巴细胞与对细胞毒性敏感的爱泼斯坦 - 巴尔病毒转化细胞合并，其在作为转移剂的敏感的EBV转化细胞被杀死并有效转化的条件下不显着影响B淋巴细胞 B淋巴细胞受体细胞与EBV。 扩增和克隆EBV转化的B淋巴细胞细胞，根据常规技术分离所需的克隆，然后用于生产单克隆抗体。

公开(公告)号：US4834975A

公开(公告)日：1989-05-30

申请号：US734624

申请日：1985-05-15

申请人： Anthony W. Siadak ,  Mark E. Lostrom

发明人： Anthony W. Siadak ,  Mark E. Lostrom

IPC分类号： G01N33/569 ,  A61K38/00 ,  A61K39/395 ,  C07K14/005 ,  C07K14/195 ,  C07K16/00 ,  C07K16/12 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  G01N33/577

CPC分类号： C07K16/1203 ,  A61K38/00 ,  C07K2317/77 ,  Y10S424/803 ,  Y10S424/808 ,  Y10S435/948 ,  Y10S530/865

摘要： Transformed human lymphocyte cell lines have been produced which secrete human monoclonal antibodies to serotypic determinants on LPS molecules of gram-negative bacteria. These antibodies have been found to be protective against lethal challenges of the homologous bacteria. Pharmaceutical compositions containing these antibodies and their prophylactic and therapeutic use in the management of gram-negative disease in humans are also disclosed.Human lymphocyte cell lines secreting human monoclonal antibodies specifically reacting with Pseudomonas aeruginosa Fisher immunotypes 1 (C5B7), 2 (6F11), 4 (C5D5), 5 (13C1), 6 (5G2) and 7 (8E7) were deposited at the American Type Culture Collection and given A.T.C.C. Accession Nos. CRL 8753, CRL 8562, CRL 8754, CRL 8796, CRL 8797 and CRL 8795, respectively.

摘要翻译： 已经产生了转化的人淋巴细胞系，其分泌针对革兰氏阴性细菌的LPS分子的血清型决定簇的人单克隆抗体。 已经发现这些抗体对于同源细菌的致死性攻击是有保护作用的。 还公开了含有这些抗体的药物组合物及其在人类革兰氏阴性病的治疗中的预防和治疗用途。 分泌人类单克隆抗体的人类淋巴细胞细胞系与绿脓杆菌特异性反应Fisher免疫型1（C5B7），2（6F11），4（C5D5），5（13C1），6（5G2）和7（8E7） 培养物收集和给予ATCC 登录号CRL 8753，CRL 8562，CRL 8754，CRL 8796，CRL 8797和CRL 8795。