公开(公告)号：US20210302316A1

公开(公告)日：2021-09-30

申请号：US17211924

申请日：2021-03-25

Applicant: Leica Microsystems CMS GmbH

Inventor： Kai WALTER ,  Lars FRIEDRICH

IPC: G01N21/64 ,  G02B21/00

Abstract: A method for estimating a stimulated emission depletion microscopy (STED) resolution includes generating a first frame representing a reference image from a field-of-view, the reference image having a predetermined reference resolution, and generating at least one second frame representing a STED image from the same field-of-view, the STED image having the STED resolution to be estimated. The at least one second frame is blurred by applying a convolution kernel with at least one fit parameter to the second frame. An optimal value of the at least one fit parameter of the convolution kernel is determined for which a difference between the first frame and the blurred at least one second frame is minimized. The STED resolution is estimated based on the optimal value of the at least one fit parameter and the predetermined reference resolution.

公开(公告)号：US20230222660A1

公开(公告)日：2023-07-13

申请号：US18152208

申请日：2023-01-10

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Soeren ALSHEIMER ,  Kai WALTER ,  Joachim BRADL

IPC: G06T7/00 ,  H04N13/207 ,  G02B21/36 ,  G01N33/50

CPC classification number: G06T7/0014 ,  H04N13/207 ,  G02B21/365 ,  G01N33/5094 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30096 ,  G06T2200/04 ,  G06T2207/30204

Abstract: A method for matching a three-dimensional first image of at least one discrete entity with a three-dimensional second image of the at least one discrete entity is provided. The at least one discrete entity includes a biological sample and a plurality of constituent parts of a marker. The method includes: generating a first representation of the marker from the first image; generating a second representation of the marker from the second image; and based upon the representations matching, matching the first image with the second image; or based upon the representations not matching, rejecting the match. Generating the representations includes determining vectors from at least one reference item to at least some of the constituent parts of the marker, determining for the vectors at least one value of a property, and generating the representations of the marker based on a frequency of the at least one value of the property.

公开(公告)号：US20220253983A1

公开(公告)日：2022-08-11

申请号：US17621710

申请日：2020-06-19

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Kai WALTER ,  Florian ZIESCHE

IPC: G06T5/00 ,  G06V10/30 ,  G06T5/20

Abstract: A signal processing apparatus for enhancing a digital input signal (I(xi)) recorded by a recording system having a system response (H(xi)), is configured to retrieve the digital input signal (I(xi)) and compute a baseline estimate (ƒ(xi)) of the digital input signal, the baseline estimate representing a baseline of the digital input signal and comprising features of the digital input signal that are larger than a feature length (fl). The apparatus is further configured to remove the baseline estimate from the digital input signal to obtain an output signal comprising spatial features that are smaller than the feature length, retrieve a characteristic length (cl) of the system response (H(xi)) and compute the baseline estimate (ƒ(xi)) using a feature length that is smaller than the characteristic length of the system response (H(xi)).

公开(公告)号：US20240255425A1

公开(公告)日：2024-08-01

申请号：US18290315

申请日：2021-12-23

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Soeren ALSHEIMER ,  Kai WALTER ,  Joachim BRADL

IPC: G01N21/64 ,  C12Q1/44

CPC classification number: G01N21/6428 ,  C12Q1/44 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2333/922

Abstract: A marker for marking a predetermined structure within a biological sample includes a marker base having an affinity reagent, and an attachment structure connected to the affinity reagent having two attachment sites. The attachment structure includes a cleavage site arranged between the attachment sites. The attachment structure is capable of being cut at the cleavage site by a cleaving agent in order to remove an attachment site from the marker base. The marker further includes at least two reporters. Each reporter includes a linker structure having a complementary attachment site configured to attach to one of the attachment sites, and a combination of at least two different fluorescent dyes. The combination of the at least two different fluorescent dyes is unique for each reporter. The complementary attachment site is unique for each reporter and configured such that each reporter attaches to a different attachment site of the marker base.

公开(公告)号：US20210118101A1

公开(公告)日：2021-04-22

申请号：US17042194

申请日：2019-03-29

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Kai WALTER ,  Florian ZIESCHE

IPC: G06T5/00 ,  G06T5/10

Abstract: An apparatus for baseline estimation in input signal data is configured to retrieve input signal data (I(xi)) and to subtract baseline estimation data (f(xi)) from the input signal data (I(xi)) to compute output signal data. The apparatus is further configured to compute the baseline estimation data (f(xi)) from a convolution using a discrete Green's function (G(xi)).

公开(公告)号：US20240303785A1

公开(公告)日：2024-09-12

申请号：US18271045

申请日：2022-01-14

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Kai WALTER

IPC: G06T5/73

CPC classification number: G06T5/73 ,  G06T2207/10064 ,  G06T2207/20081

Abstract: A data processing apparatus for processing a digital input image is configured to receive the digital input image. The digital input image includes input photon arrival-time data at input image locations. The data processing apparatus is further configured to compute a digital output image based on the digital input image by deconvolution. The digital output image includes output photon arrival-time data at output image locations. The output photon arrival-time data represent an estimate of an unblurred ground-truth of the input photon arrival-time data. The data processing apparatus is further configured to compute the deconvolution by an iterative algorithm using an update function. The update function depends on a point-spread function, a previous estimate of the ground-truth, and the input photon arrival-time data.

公开(公告)号：US20230410937A1

公开(公告)日：2023-12-21

申请号：US18332001

申请日：2023-06-09

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Soeren ALSHEIMER ,  Joachim BRADL ,  Kai WALTER

IPC: G16B5/20 ,  G01N21/64

CPC classification number: G16B5/20 ,  G01N21/6428 ,  G01N2021/6441

Abstract: A method for analysing a biological sample with analytes includes determining information about the biological sample and the analytes. The analytes are marked by a plurality of markers. The method further includes generating a probabilistic model of a distribution of the analytes within the biological sample based on the determined information, generating at least one optical readout of the biological sample, and determining presence of at least one analyte in the at least one optical readout based at least partially on the probabilistic model of the distribution of the analytes within the biological sample.

公开(公告)号：US20220327670A1

公开(公告)日：2022-10-13

申请号：US17625099

申请日：2020-07-08

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Florian ZIESCHE ,  Kai WALTER

IPC: G06T5/00

Abstract: A signal processing apparatus for deblurring a digital input signal (I(xi)) comprising one or more processors. The one or more processors are configured to: compute a plurality of local length scales (lk, l(xi), λ) from at least one of a local signal resolution (FRCk) and a local signal-to-noise ratio (SNRk), and to compute each of the local length scales at a different location (Ik(xi)) of the digital input signal, each of the different locations comprising at least one sample point (xi) of the input signal; compute a baseline estimate (ƒn(xi, ln), ƒ(xi, l(xi))) of the input signal based on the local length scales, the baseline estimate representing signal structures of the digital input signal that are larger than the local length scale; and compute a digital output signal (O(xi)) based on one of (a) the baseline estimate and (b) the digital input signal and the baseline estimate.

公开(公告)号：US20210118102A1

公开(公告)日：2021-04-22

申请号：US17042195

申请日：2019-03-29

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Kai WALTER ,  Florian ZIESCHE

IPC: G06T5/00 ,  G06T5/20 ,  H04L25/02

Abstract: A method for estimating baseline in a signal, the signal being represented by input signal data (I(xi)), includes estimating a baseline contribution (I2(xi)) in the signal to obtain baseline estimation data (f(xi)), wherein the baseline estimation data (f(xi)) are computed as a fit to at least a subset of the input signal data (I(xi)) by minimizing a least-square minimization criterion (M(f(xi))). Deblurred output signal data (O(xi)) are obtained based on the baseline estimation data (f(xi)) and the input signal data (I(xi)). The least-square minimization criterion (M(f(xi))) comprises a penalty term (P(f(xi))).

公开(公告)号：US20250022102A1

公开(公告)日：2025-01-16

申请号：US18768102

申请日：2024-07-10

Applicant: Leica Microsystems CMS GmbH

Inventor： Oliver SCHLICKER ,  Markus SCHECHTER ,  Kai WALTER

IPC: G06T5/50 ,  G06T5/60 ,  G06T7/00

Abstract: The invention relates to a data processing device for an imaging apparatus, the device being configured to obtain a length threshold value, to access input image data representing at least one digital input image, wherein the input image data comprise a shading signal representing a brightness decrease towards the edges of the at least one digital input image, and a content signal representing image features of the at least one digital input image, the image features having a length that is smaller than the length threshold value, to compute a baseline image based on the input image data and the length threshold value, wherein the baseline image is representative of an estimate of the shading signal, to generate at least one digital output image representative of an estimate of the content signal by at least one of a) subtracting the baseline image from the input image data and b) dividing the input image data by the baseline image. Advantageously, the data processing device allows a shading correction without the need for obtaining any separate background image or reducing the image size. The invention also relates to a computer-implemented method and a method for operating such an imaging apparatus. Further, the invention relates to a computer program and a computer-readable medium. Lastly, the invention relates to a neural network device trained by means of the data processing device or the computer-implemented method.