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    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME
    1.
    发明公开
    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME 审中-公开

    公开(公告)号：US20240101980A1

    公开(公告)日：2024-03-28

    申请号：US18521466

    申请日：2023-11-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel MAZUR ,  Sihong CHEN ,  Peter VANDER HORN ,  Eileen TOZER ,  Guobin LUO ,  Joshua SHIRLEY ,  Kevin HEINEMANN

    IPC: C12N9/12 ,  C12Q1/686

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    2.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开

    公开(公告)号：US20170260512A1

    公开(公告)日：2017-09-14

    申请号：US15481858

    申请日：2017-04-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter VANDER HORN ,  Daniel MAZUR ,  Theo NIKIFOROV ,  Mindy LANDES ,  Eileen TOZER

    IPC: C12N9/12 ,  C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    3.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开

    公开(公告)号：US20200277580A1

    公开(公告)日：2020-09-03

    申请号：US16850629

    申请日：2020-04-16

    Applicant: Life Technologies Corporation

    Inventor： Peter VANDER HORN ,  Theo NIKIFOROV ,  Guobin LUO ,  Mindy LANDES ,  Daniel MAZUR ,  Eileen TOZER ,  Tommie LINCECUM

    IPC: C12N9/12 ,  C12Q1/6844 ,  C12Q1/686

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR
    5.
    发明申请
    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR 有权

    公开(公告)号：US20220315917A1

    公开(公告)日：2022-10-06

    申请号：US17302674

    申请日：2021-05-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark ANDERSEN ,  Daniel MAZUR ,  Sihong CHEN ,  Guobin LUO ,  Xinzhan PENG

    IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6855

    Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    6.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开

    公开(公告)号：US20200299655A1

    公开(公告)日：2020-09-24

    申请号：US16894469

    申请日：2020-06-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter VANDER HORN ,  Daniel MAZUR ,  Theo NIKIFOROV ,  Mindy LANDES ,  Eileen TOZER

    IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME
    7.
    发明申请
    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME 审中-公开

    公开(公告)号：US20190270974A1

    公开(公告)日：2019-09-05

    申请号：US16415063

    申请日：2019-05-17

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel MAZUR ,  Peter VANDER HORN ,  Eileen TOZER ,  Sihong CHEN ,  Guobin LUO ,  Joshua SHIRLEY ,  Kevin HEINEMANN

    IPC: C12N9/12 ,  C12Q1/686

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

    POLYMERASE COMPOSITIONS AND METHODS
    9.
    发明申请
    POLYMERASE COMPOSITIONS AND METHODS 有权
    聚合物组合物和方法

    公开(公告)号：US20150247136A1

    公开(公告)日：2015-09-03

    申请号：US14710160

    申请日：2015-05-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Stephen P. HENDRICKS ,  Michael PHELAN ,  Marian PERIS ,  Cheng-Yao CHEN ,  Daniel MAZUR ,  Xinzhan PENG ,  Amy CASTILLO

    IPC: C12N9/12

    CPC classification number: C12Q1/6869 ,  C07H19/20 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6818 ,  C12Y207/07 ,  C12Y207/07007 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6432

    Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.

    Abstract translation: 本文公开了显示改变的聚合酶活性的修饰聚合酶组合物，其可用于多种生物应用。 本文还公开了制备和使用这些组合物的方法。 在一些实施方案中，组合物显示改变的性质，其可以增强其在各种生物应用中的效用。 这种改变的性质可以包括例如改变的核苷酸结合亲和力，改变的核苷酸掺入动力学，改变的光稳定性和/或改变的纳米颗粒耐受性，以及本文公开的一系列其它性质。

    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    10.
    发明公开
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开

    公开(公告)号：US20240067941A1

    公开(公告)日：2024-02-29

    申请号：US18486337

    申请日：2023-10-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter VANDER HORN ,  Theo NIKIFOROV ,  Daniel MAZUR ,  Eileen TOZER ,  Mindy LANDES

    IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6869

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

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