公开(公告)号：US20200277580A1

公开(公告)日：2020-09-03

申请号：US16850629

申请日：2020-04-16

Applicant: Life Technologies Corporation

Inventor： Peter VANDER HORN ,  Theo NIKIFOROV ,  Guobin LUO ,  Mindy LANDES ,  Daniel MAZUR ,  Eileen TOZER ,  Tommie LINCECUM

IPC: C12N9/12 ,  C12Q1/6844 ,  C12Q1/686

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

公开(公告)号：US20240101980A1

公开(公告)日：2024-03-28

申请号：US18521466

申请日：2023-11-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel MAZUR ,  Sihong CHEN ,  Peter VANDER HORN ,  Eileen TOZER ,  Guobin LUO ,  Joshua SHIRLEY ,  Kevin HEINEMANN

IPC: C12N9/12 ,  C12Q1/686

CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

公开(公告)号：US20170260512A1

公开(公告)日：2017-09-14

申请号：US15481858

申请日：2017-04-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Peter VANDER HORN ,  Daniel MAZUR ,  Theo NIKIFOROV ,  Mindy LANDES ,  Eileen TOZER

IPC: C12N9/12 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

公开(公告)号：US20200299655A1

公开(公告)日：2020-09-24

申请号：US16894469

申请日：2020-06-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Peter VANDER HORN ,  Daniel MAZUR ,  Theo NIKIFOROV ,  Mindy LANDES ,  Eileen TOZER

IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

公开(公告)号：US20190270974A1

公开(公告)日：2019-09-05

申请号：US16415063

申请日：2019-05-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel MAZUR ,  Peter VANDER HORN ,  Eileen TOZER ,  Sihong CHEN ,  Guobin LUO ,  Joshua SHIRLEY ,  Kevin HEINEMANN

IPC: C12N9/12 ,  C12Q1/686

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

公开(公告)号：US20170096648A1

公开(公告)日：2017-04-06

申请号：US15277834

申请日：2016-09-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： DANIEL MAZUR ,  Peter VANDER HORN ,  Eileen TOZER ,  Sihong CHEN ,  Guobin LUO ,  Joshua SHIRLEY ,  Kevin HEINEMANN

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007 ,  C12Q2521/101

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

公开(公告)号：US20240067941A1

公开(公告)日：2024-02-29

申请号：US18486337

申请日：2023-10-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Peter VANDER HORN ,  Theo NIKIFOROV ,  Daniel MAZUR ,  Eileen TOZER ,  Mindy LANDES

IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6869

CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

公开(公告)号：US20150094211A1

公开(公告)日：2015-04-02

申请号：US14502201

申请日：2014-09-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Peter VANDER HORN ,  Daniel MAZUR ,  Theo NIKIFOROV ,  Mindy LANDES ,  Eileen TOZER

IPC: C12N9/12 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

Abstract translation: 本公开提供了可用于核酸聚合的组合物，方法，试剂盒，系统和装置。 特别地，提供了允许核酸扩增的修饰的聚合酶及其生物活性片段。 在一些方面，本公开提供与参考聚合酶相比具有较低系统误差的修饰聚合酶。 一方面，本公开涉及可用于核酸测序，基因分型，拷贝数变异分析，配对末端测序和其他形式遗传分析的修饰聚合酶。 在一些方面，本公开涉及可用于产生核酸文库或核酸模板的修饰聚合酶。 在一些方面，本公开涉及可以跨越聚合酶的类或家族转移的同源氨基酸突变的鉴定，以提供具有改变的性质的新型聚合酶。

公开(公告)号：US20220162573A1

公开(公告)日：2022-05-26

申请号：US17645146

申请日：2021-12-20

Applicant: Life Technologies Corporation

Inventor： Peter VANDER HORN ,  Theo NIKIFOROV ,  Guobin LUO ,  Mindy LANDES ,  Daniel MAZUR ,  Eileen TOZER ,  Tommie LINCECUM

IPC: C12N9/12 ,  C12Q1/6844 ,  C12Q1/686

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

公开(公告)号：US20210371834A1

公开(公告)日：2021-12-02

申请号：US17445047

申请日：2021-08-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Peter VANDER HORN ,  Daniel MAZUR ,  Theo NIKIFOROV ,  Mindy LANDES ,  Eileen TOZER

IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.