公开(公告)号：US20210062158A1

公开(公告)日：2021-03-04

申请号：US16839906

申请日：2020-04-03

Applicant: Kyoto University ,  Megakaryon Corporation

Inventor： Koji Eto ,  Hiroshi Endo ,  Tomohiro Shigemori

IPC: C12N5/074 ,  C12N5/078

Abstract: Provided is a method for producing a stem cell clone, which comprises the steps of: (i) introducing into stem cells an exogenous gene associated with induction of differentiation into somatic cells; (ii) inducing differentiation of the stem cells, introduced with an exogenous gene, into the somatic cells; (iii) dedifferentiating the differentiation-induced somatic cells; and (iv) isolating stem cells having the exogenous gene incorporated into a chromosome thereof from a colony of the stem cells formed in step (iii).

公开(公告)号：US20190032015A1

公开(公告)日：2019-01-31

申请号：US16071598

申请日：2017-01-30

Applicant: Kyoto University ,  MEGAKARYON CORPORATION

Inventor： Koji Eto ,  Hideya Seo ,  Akira Ota ,  Yukitaka Ito ,  Yasuko Hazama

IPC: C12N5/078

Abstract: The present invention provides: a platelet production promoter that contains one or more substances selected from the group consisting of Wnt inhibitors and FMS-like tyrosine kinase (FLT) inhibitors; and a platelet production method that uses this platelet production promoter.

公开(公告)号：US11976301B2

公开(公告)日：2024-05-07

申请号：US17209035

申请日：2021-03-22

Applicant: Megakaryon Corporation ,  Kyoto University

Inventor： Takeaki Dohda ,  Sachiko Kobayashi ,  Koji Eto ,  Hiroshi Endo ,  Sou Nakamura

IPC: C12N5/00 ,  C12N5/078

CPC classification number: C12N5/0644 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2506/45 ,  C12N2510/00

Abstract: The present invention provides a method for producing megakaryocytes that have an increased capacity to produce platelets, the method comprising a step of culturing, under conditions that cause the death of cells that do not express a gene that is specifically expressed by megakaryocytes, cells that have the capacity to differentiate into megakaryocytes.

公开(公告)号：US11136547B2

公开(公告)日：2021-10-05

申请号：US16083503

申请日：2017-03-22

Applicant: Kyoto University ,  Megakaryon Corporation

Inventor： Koji Eto ,  Hiroshi Endo ,  Yukitaka Ito ,  Junichi Fukunaga

IPC: A61K35/19 ,  A61K35/28 ,  A61K35/545 ,  C12N5/0735 ,  C12N5/078 ,  C12N5/00 ,  C12N5/10 ,  C12N15/09 ,  A61P7/08 ,  A61K38/18 ,  C12N5/074

Abstract: Provided is a method for inducing mesoderm, comprising a step of bringing pluripotent stem cells into contact with bone morphogenetic protein 4 (BMP4) or CHIR for at least 3 days.

公开(公告)号：US20210222124A1

公开(公告)日：2021-07-22

申请号：US17209035

申请日：2021-03-22

Applicant: Megakaryon Corporation ,  Kyoto University

Inventor： Takeaki Dohda ,  Sachiko Kobayashi ,  Koji Eto ,  Hiroshi Endo ,  Sou Nakamura

IPC: C12N5/078

Abstract: The present invention provides a method for producing megakaryocytes that have an increased capacity to produce platelets, the method comprising a step of culturing, under conditions that cause the death of cells that do not express a gene that is specifically expressed by megakaryocytes, cells that have the capacity to differentiate into megakaryocytes.

公开(公告)号：US10941382B2

公开(公告)日：2021-03-09

申请号：US16071598

申请日：2017-01-30

Applicant: Kyoto University ,  Megakaryon Corporation

Inventor： Koji Eto ,  Hideya Seo ,  Akira Ota ,  Yukitaka Ito ,  Yasuko Hazama

IPC: C12N5/078 ,  A61P7/04 ,  A61K31/444 ,  A61K31/381 ,  C12N15/09 ,  A61K35/19

Abstract: The present invention provides: a platelet production promoter that contains one or more substances selected from the group consisting of Wnt inhibitors and FMS-like tyrosine kinase (FLT) inhibitors; and a platelet production method that uses this platelet production promoter.

公开(公告)号：US20180051260A1

公开(公告)日：2018-02-22

申请号：US15565922

申请日：2016-04-14

Applicant: Kyoto University ,  Megakaryon Corporation

Inventor： Koji Eto ,  Hiroshi Endo ,  Tomohiro Shigemori

IPC: C12N5/074 ,  C12N5/078

CPC classification number: C12N5/0696 ,  C12N5/0644 ,  C12N2506/11 ,  C12N2506/45 ,  C12N2510/00

Abstract: Provided is a method for producing a stem cell clone, which comprises the steps of: (i) introducing into stem cells an exogenous gene associated with induction of differentiation into somatic cells; (ii) inducing differentiation of the stem cells, introduced with an exogenous gene, into the somatic cells; (iii) dedifferentiating the differentiation-induced somatic cells; and (iv) isolating stem cells having the exogenous gene incorporated into a chromosome thereof from a colony of the stem cells formed in step (iii).

公开(公告)号：US20180044634A1

公开(公告)日：2018-02-15

申请号：US15555626

申请日：2016-03-09

Applicant: Megakaryon Corporation ,  Kyoto University

Inventor： Takeaki Dohda ,  Sachiko Kobayashi ,  Koji Eto ,  Hiroshi Endo ,  Sou Nakamura

IPC: C12N5/078

CPC classification number: C12N5/0644 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2506/45 ,  C12N2510/00

Abstract: The present invention provides a method for producing megakaryocytes that have an increased capacity to produce platelets, the method comprising a step of culturing, under conditions that cause the death of cells that do not express a gene that is specifically expressed by megakaryocytes, cells that have the capacity to differentiate into megakaryocytes.

公开(公告)号：US20240026304A1

公开(公告)日：2024-01-25

申请号：US18476065

申请日：2023-09-27

Applicant: Kyoto University ,  Megakaryon Corporation

Inventor： Koji Eto ,  Hiroshi Endo ,  Tomohiro Shigemori

IPC: C12N5/074 ,  C12N5/078

CPC classification number: C12N5/0696 ,  C12N5/0644 ,  C12N2506/11 ,  C12N2506/45 ,  C12N2510/00

Abstract: Provided is a method for producing a stem cell clone, which comprises the steps of: (i) introducing into stem cells an exogenous gene associated with induction of differentiation into somatic cells; (ii) inducing differentiation of the stem cells, introduced with an exogenous gene, into the somatic cells; (iii) dedifferentiating the differentiation-induced somatic cells; and (iv) isolating stem cells having the exogenous gene incorporated into a chromosome thereof from a colony of the stem cells formed in step (iii).

公开(公告)号：US11016083B2

公开(公告)日：2021-05-25

申请号：US16071716

申请日：2017-01-30

Applicant: Kyoto University ,  Megakaryon Corporation

Inventor： Koji Eto ,  Hideya Seo ,  Akira Ota ,  Yukitaka Ito ,  Yasuko Hazama

IPC: C12N15/09 ,  C12Q1/6897 ,  G01N33/50 ,  C12N15/10

Abstract: The present invention provides a method for screening for platelet production promoters, the method including a step for selecting a candidate substance that significantly increases expression of TUBB1 as a platelet production promoter.