公开(公告)号：US09193984B2

公开(公告)日：2015-11-24

申请号：US13733687

申请日：2013-01-03

Applicant: Korea Basic Science Institute

Inventor： Zee Won Lee ,  Soo Hyun Kim

IPC: G01N33/53 ,  C12Q1/02 ,  G01N33/50 ,  G01N33/542 ,  G01N33/557 ,  C12N15/10

CPC classification number: C12Q1/025 ,  C07K2319/01 ,  C07K2319/60 ,  C12N15/1055 ,  G01N33/5035 ,  G01N33/542 ,  G01N33/557

Abstract: Disclosed is a novel method for detecting interactions of biomolecules. More particularly, the disclosed method includes (a) preparing a cell comprising (i) a first construct comprising a bait, a first labeling material and a translocation module; and (ii) a second construct comprising a prey and a second labeling material; (b) detecting the distribution of the first construct and the second construct in the cell. the present invention provides a method capable of detecting bindings and interactions occurring in a living cell in real time, and a method for screening a material that alters the binding and the interaction. The method of the present invention overcomes the disadvantages including inaccuracy and complexity of existing biomaterial interaction detection techniques. By labeling both constructs to promote accuracy, the present invention provides a novel real-time, antibody-free analysis.

Abstract translation: 公开了一种检测生物分子相互作用的新方法。 更具体地，所公开的方法包括（a）制备细胞，其包括（i）包含诱饵，第一标记材料和易位模块的第一构建体; 和（ii）包含猎物和第二标签材料的第二构建体; （b）检测细胞中第一构建体和第二构建体的分布。 本发明提供能够实时检测活细胞中发生的结合和相互作用的方法，以及筛选改变结合和相互作用的材料的方法。 本发明的方法克服了现有生物材料相互作用检测技术的不准确性和复杂性的缺点。 通过标记两种构建体以促进准确性，本发明提供了一种新颖的实时抗体免疫分析。

公开(公告)号：US20130130273A1

公开(公告)日：2013-05-23

申请号：US13733687

申请日：2013-01-03

Applicant: Korea Basic Science Institute

Inventor： Zee Won Lee ,  Soo Hyun Kim

IPC: C12Q1/02 ,  G01N33/53

CPC classification number: C12Q1/025 ,  C07K2319/01 ,  C07K2319/60 ,  C12N15/1055 ,  G01N33/5035 ,  G01N33/542 ,  G01N33/557

Abstract: Disclosed is a novel method for detecting interactions of biomolecules. More particularly, the disclosed method includes (a) preparing a cell comprising (i) a first construct comprising a bait, a first labeling material and a translocation module; and (ii) a second construct comprising a prey and a second labeling material; (b) detecting the distribution of the first construct and the second construct in the cell. the present invention provides a method capable of detecting bindings and interactions occurring in a living cell in real time, and a method for screening a material that alters the binding and the interaction. The method of the present invention overcomes the disadvantages including inaccuracy and complexity of existing biomaterial interaction detection techniques. By labeling both constructs to promote accuracy, the present invention provides a novel real-time, antibody-free analysis.

Abstract translation: 公开了一种检测生物分子相互作用的新方法。 更具体地，所公开的方法包括（a）制备细胞，其包括（i）包含诱饵，第一标记材料和易位模块的第一构建体; 和（ii）包含猎物和第二标签材料的第二构建体; （b）检测细胞中第一构建体和第二构建体的分布。 本发明提供能够实时检测活细胞中发生的结合和相互作用的方法，以及筛选改变结合和相互作用的材料的方法。 本发明的方法克服了现有生物材料相互作用检测技术的不准确性和复杂性的缺点。 通过标记两种构建体以促进准确性，本发明提供了一种新颖的实时抗体免疫分析。