公开(公告)号：US20230091480A1

公开(公告)日：2023-03-23

申请号：US17931711

申请日：2022-09-13

Applicant: Korea Advanced Institute of Science and Technology

Inventor： Jae-Byum JANG ,  Hyunwoo KIM ,  Young-Gyu YOON ,  Hoyeon NAM ,  Junyoung SEO

IPC: G01N21/64

Abstract: The present disclosure relates to a method and device for unmixing fluorescent signals based on a joint histogram. The method and device may be configured to obtain a plurality of images of a plurality of fluorescent materials marked in different biomolecules and to unmix the signals of the fluorescent materials based on at least one joint histogram index defined between the images of each of pairs each including two or more of the obtained images. According to the present disclosure, the joint histogram index may include at least one of Kullback-Leibler divergence, cross-entropy, or Rand-index.

公开(公告)号：US20220215517A1

公开(公告)日：2022-07-07

申请号：US17567300

申请日：2022-01-03

Applicant: Korea Advanced Institute of Science and Technology

Inventor： JaeByum CHANG ,  Junyoung SEO ,  Young-Gyu YOON ,  Hyunwoo KIM ,  Jee Won KIM

IPC: G06T5/50 ,  G06T3/40 ,  G06T7/90 ,  G01N21/64

Abstract: This disclosure provides a method and apparatus for multicolor unmixing through iterative mutual information minimization in a simultaneous channel update structure. In this disclosure, a plurality of images may be obtained with respect to a plurality of fluorophores labeled on different biomolecules, respectively. Images of the fluorophores may be unmixed from the obtained images, respectively, while simultaneously minimizing mutual information shared between the obtained images based on all permutation combinations of the obtained images. According to the present disclosure, signals having three or more fluorophores whose emission spectra are overlapped in one spectral range can be more accurately unmixed.

公开(公告)号：US20240257346A1

公开(公告)日：2024-08-01

申请号：US18602661

申请日：2024-03-12

Applicant: Korea Advanced Institute Of Science and Technology

Inventor： JaeByum CHANG ,  Hyunwoo KIM ,  Junyoung SEO ,  Young-Gyu YOON ,  Hoyeon NAM

IPC: G06T7/00

CPC classification number: G06T7/0012 ,  G06T2207/10064 ,  G06T2207/20081 ,  G06T2207/20084

Abstract: As an aspect of the present disclosure, a method of processing an image may be proposed. The method is a method of processing an image, which is performed by an electronic device including one or more processors and one or more memories in which instructions to be executed by the one or more processors are stored, and may include obtaining a first mixed image of a sample including a first biomolecule labeled with a first fluorescent material and a second biomolecule that has not been labeled, obtaining a second mixed image of the sample including the first biomolecule labeled with the first fluorescent material and the second biomolecule labeled with a second fluorescent material, and generating an unmixed image of the second biomolecule based on the first mixed image and the second mixed image.

公开(公告)号：US20220113314A1

公开(公告)日：2022-04-14

申请号：US17495133

申请日：2021-10-06

Applicant: Korea Advanced Institute Of Science And Technology

Inventor： Jae-Byum CHANG ,  Yehlin CHO ,  Haemin YEON ,  Junyoung SEO ,  Yeonbo SIM

IPC: G01N33/58 ,  G01N33/53 ,  G01N21/64

Abstract: Various embodiments can provide a method of amplifying a fluorescence signal through the cyclic staining of complementary antibodies conjugated with fluorophores. According to various embodiments, the method of amplifying a fluorescence signal may be configured to prepare a primary antibody and antibody pairs with respect to each target protein, bind the primary antibody to the target protein, and bind the antibody pairs to the primary antibody. The multicolor fluorescence signals may be amplified and fabricated by forming fluorescence signals in a way to prepare different antibodies for which cross reactions with different types of target proteins, respectively, have been removed using an agarose gel and to bind different antibody pairs according to combinations of different antibodies to different primary antibodies bound to target proteins, respectively.

公开(公告)号：US20220065788A1

公开(公告)日：2022-03-03

申请号：US17445629

申请日：2021-08-23

Applicant: Korea Advanced Institute Of Science And Technology

Inventor： Jae-Byum CHANG ,  Young-Gyu YOON ,  Junyoung SEO ,  Yeonbo SIM ,  Jee Won KIM

IPC: G01N21/64

Abstract: The present disclosure provides a method and apparatus for biomolecular multiplexed imaging through the iterative unmixing of fluorophores. According to the first embodiments of the present disclosure, although signals of two fluorophores are detected in the first fluorescent detection spectral range, the signals of the two images obtained from two detection spectral ranges can be unmixed through the iterative minimization of mutual information. Furthermore, the present disclosure provides a multi-color unmixing method and apparatus through the iterative minimization of mutual information. In the second embodiments of the present disclosure, a plurality of images of a plurality of fluorophores marking different biomolecules, respectively, for example, N fluorophores are obtained. Images, each containing the signals of single fluorophore can be obatined from the obtained images while minimizing the mutual information shared by images of each of pairs each consisting of two of the obtained images.