公开(公告)号：US20210170342A1

公开(公告)日：2021-06-10

申请号：US16770899

申请日：2018-12-13

Applicant: King Abdullah University of Science and Technology

Inventor： Giada SOLDAN ,  Suzana NUNES

IPC: B01D69/02 ,  B01D71/80 ,  B01D71/02 ,  B01D71/28 ,  B01D15/38 ,  B01D15/34 ,  B01D15/26 ,  C07K1/16

Abstract: Embodiments of the present disclosure describe functionalized polymeric membranes including one or more dithiol compounds that extend from a nanoparticle provided on or near a surface and/or pores of a polymer material, wherein at least one thiol of the dithiol compound binds to the nanoparticle and at least one thiol of the dithiol compound is a free thiol. Embodiments of the present disclosure further describe methods of separating and/or recovering a purified antibody comprising contacting a feed stream containing an antibody and other biomolecules with a functionalized polymeric membrane to separate the antibody from the feed stream; and applying a reducing agent to release the antibody from the membrane and recover a purified antibody; wherein the functionalized polymeric membrane includes a plurality of free thiols selective to binding the antibody.