公开(公告)号：US20220326155A1

公开(公告)日：2022-10-13

申请号：US17623244

申请日：2021-11-11

申请人： JIANGNAN UNIVERSITY

发明人： Yi ZHANG ,  Yongxi HE ,  Wenlong WANG ,  Lingling WANG ,  Xiuping YAN

IPC分类号： G01N21/64 ,  G01N33/533 ,  G01N33/577 ,  G01N33/58

摘要： A time-resolved fluorescence and chromogenic dual-signal test strip for estrogen is based on the principle of immune recognition and fluorescence resonance energy transfer. In time-resolved fluorescence mode, estrogen-BSA-persistent luminescence particle complex is the fluorescence donor, and colloidal gold modified with estrogen monoclonal antibody is the fluorescence receptor, which is also chromogenic signal unit in the chromogenic mode. The photos of strips in both modes are obtained with smart phones. The time-resolved fluorescence intensity of the test strip test zone is positively correlated with estrogen content, and the chromogenic intensity is negatively correlated with estrogen content. The competitive time-resolved fluorescence and chromogenic dual-signal immunochromatographic test strips can accurately and quickly detect estrogen and estrogen-like compounds.

公开(公告)号：US20230204545A1

公开(公告)日：2023-06-29

申请号：US18147248

申请日：2022-12-28

申请人： JIANGNAN UNIVERSITY

发明人： Yi ZHANG ,  Haodong Wei ,  Yongwei FENG ,  Yongxi HE ,  Chenhui LU ,  Lizhi Xu ,  Wenlong WANG ,  Xiaofang SHEN ,  Xiuping YAN

IPC分类号： G01N30/72 ,  G01N30/86 ,  G01N33/02 ,  B01D15/38

CPC分类号： G01N30/7233 ,  G01N30/8631 ,  G01N33/02 ,  B01D15/3804 ,  B01D15/388 ,  G01N2030/027

摘要： The invention discloses a method for the detection of sialate glycosyl casein glycomacropeptide by boronate affinity column enrichment-liquid chromatography-tandem mass spectrometry using phenylboric acid modified mesoporous silica as packing material, which belongs to the field of food analysis and detection. The method includes the following steps: (1) sample preparation; (2) enrichment and purification of boronate affinity column; (3) liquid chromatography-tandem mass spectrometry detection. The invention makes use of the affinity property of phenylboric acid to the special sugar group sialic acid on the serine and threonine residues in casein glycogiant peptide, regulates the adsorption and elution of casein glycogiant peptide with sialic acid group by changing pH. Combined with the high sensitivity and accuracy of liquid chromatography tandem mass spectrometry, it can be used for qualitative and quantitative analysis of casein glycomacropeptide with sialate glycol-group in phenylketonuria special medical formulations with complex matrix.