公开(公告)号：US11340215B2

公开(公告)日：2022-05-24

申请号：US16079895

申请日：2017-02-27

Applicant: JAPANESE FOUNDATION FOR CANCER RESEARCH

Inventor： Toru Hirota ,  Yusuke Abe

IPC: G01N33/50 ,  C07K16/18 ,  G01N33/574 ,  G01N33/68 ,  C12N15/113

Abstract: The inventors have found that the interaction between HP1 and INCENP can serve as an indicator for chromosome instability and established a method for evaluating chromosome instability of cancer cells. The evaluation system can be used for screening of anticancer agent with a new-concept of targeting chromosome instability of cancer cells. The inventors further prepared an antibody for specifically recognizing phosphorylation of serine at position 92 of HP1α, by which the action of Aurora B can be evaluated. The interaction between HP1 and INCENP can be readily evaluated by the antibody.

公开(公告)号：US20190094207A1

公开(公告)日：2019-03-28

申请号：US16079895

申请日：2017-02-27

Applicant: JAPANESE FOUNDATION FOR CANCER RESEARCH

Inventor： Toru Hirota ,  Yusuke Abe

IPC: G01N33/50 ,  C07K16/18 ,  G01N33/574 ,  G01N33/68

Abstract: The inventors have found that the interaction between HP1 and INCENP can serve as an indicator for chromosome instability and established a method for evaluating chromosome instability of cancer cells. The evaluation system can be used for screening of anticancer agent with a new-concept of targeting chromosome instability of cancer cells. The inventors further prepared an antibody for specifically recognizing phosphorylation of serine at position 92 of HP1α, by which the action of Aurora B can be evaluated. The interaction between HP1 and INCENP can be readily evaluated by the antibody.

公开(公告)号：US20150140590A1

公开(公告)日：2015-05-21

申请号：US14413760

申请日：2013-07-10

Applicant: JAPANESE FOUNDATION FOR CANCER RESEARCH

Inventor： Toru Hirota ,  Norihisa Shindo ,  Kazuki Kumada

IPC: C12Q1/37 ,  G01N33/50

CPC classification number: C12Q1/37 ,  C07K2319/01 ,  C07K2319/50 ,  C07K2319/60 ,  C12N9/6472 ,  C12Y304/22049 ,  G01N33/5011 ,  G01N33/542 ,  G01N2333/96466 ,  G01N2500/04

Abstract: Problem to be SolvedAn object of the present invention is to provide a separase sensor for visualizing separase activity in a living cell. Another object is to develop a function analysis system of cell division using the separase sensor and further obtain a simple screening method for an anticancer agent.SolutionThe separase sensor of the present invention has two types of fluorescent proteins different in fluorescence wavelength at both ends of an amino acid sequence containing a separase cleavage site and a localization-targeting sequence targeting to a specific site within a cell. Since timing of activating separase and location of separase in a cell can be specified by use of the sensor, cell division can be analyzed and an anticancer agent can be screened.

Abstract translation: 要解决的问题本发明的目的是提供一种用于在活细胞中可视化分离酶活性的分离酶传感器。 另一个目的是开发使用分离酶传感器的细胞分裂功能分析系统，并进一步获得抗癌剂的简单筛选方法。 解决方案本发明的分离酶传感器具有两种类型的荧光蛋白，它们在含有分离酶切割位点的氨基酸序列的两端的荧光波长和靶向细胞内特定位点的定位靶向序列。 由于可以通过使用传感器来指定活化分离酶的定时和分离酶的位置，因此可以分析细胞分裂并筛选抗癌剂。

公开(公告)号：US10174359B2

公开(公告)日：2019-01-08

申请号：US14413760

申请日：2013-07-10

Applicant: JAPANESE FOUNDATION FOR CANCER RESEARCH

Inventor： Toru Hirota ,  Norihisa Shindo ,  Kazuki Kumada

IPC: C12Q1/37 ,  G01N33/50 ,  G01N33/542 ,  C12N9/64

Abstract: Problem to be SolvedAn object of the present invention is to provide a separase sensor for visualizing separase activity in a living cell. Another object is to develop a function analysis system of cell division using the separase sensor and further obtain a simple screening method for an anticancer agent.SolutionThe separase sensor of the present invention has two types of fluorescent proteins different in fluorescence wavelength at both ends of an amino acid sequence containing a separase cleavage site and a localization-targeting sequence targeting to a specific site within a cell. Since timing of activating separase and location of separase in a cell can be specified by use of the sensor, cell division can be analyzed and an anticancer agent can be screened.