公开(公告)号：US20240352403A1

公开(公告)日：2024-10-24

申请号：US18626055

申请日：2024-04-03

申请人： Hubei University

发明人： Shihui YANG ,  Mimi HU ,  Qiaoning HE

IPC分类号： C12N1/20 ,  C12P7/06 ,  C12P7/16 ,  C12P7/42 ,  C12R1/01

CPC分类号： C12N1/205 ,  C12P7/065 ,  C12P7/16 ,  C12P7/42 ,  C12R2001/01

摘要： Genetically modified Zymomonas mobilis to utilize inorganic N source, methods and uses thereof are provided. The genetically modified Z. mobilis has a genome with the knockout of a locus ZMO1107 from Z. mobilis ZM4 could ferment inorganic N source.

公开(公告)号：US20230407242A1

公开(公告)日：2023-12-21

申请号：US18146506

申请日：2022-12-27

申请人： Hubei University

发明人： Shihui YANG ,  Qiaoning HE ,  Yang LI

IPC分类号： C12N1/20 ,  C12P7/06 ,  C12P7/18

CPC分类号： C12N1/205 ,  C12P7/06 ,  C12P7/18 ,  C12R2001/01

摘要： The disclosure relates to a genetically engineered Z. mobilis ZM4 for producing PHB and uses thereof. A variety of strains capable of producing both PHB and ethanol that are constructed by integrating three codon-optimized essential genes for exogenous PUB biosynthesis, phaA, phaB and phaC, together with a tetracycline-inducible promoter Ptet into the genome of ZM4.

公开(公告)号：US20240301448A1

公开(公告)日：2024-09-12

申请号：US18401511

申请日：2023-12-31

申请人： Hubei University

发明人： Shihui YANG ,  Xiongying YAN ,  Mian LI ,  Xia WANG ,  Qiaoning HE

IPC分类号： C12P7/18 ,  C12N9/02 ,  C12N9/04 ,  C12N9/88 ,  C12N15/74

CPC分类号： C12P7/18 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/88 ,  C12N15/74 ,  C12Y101/01001 ,  C12Y101/01175 ,  C12Y102/01021 ,  C12Y401/02 ,  C12Y402/01082

摘要： Recombinant Zymomonas mobilis for producing ethylene glycol, method and uses thereof are provided. The recombinant Zymomonas mobilis carries and expresses genes related to a synthesis pathway of xylonic acid and genes related to a synthesis pathway of ethylene glycol.

公开(公告)号：US20240093247A1

公开(公告)日：2024-03-21

申请号：US18468813

申请日：2023-09-18

申请人： Hubei University

发明人： Shihui YANG ,  Binan GENG ,  Qiaoning HE ,  Jun DU ,  Mian LI

IPC分类号： C12P7/10 ,  C12N9/22 ,  C12N15/11 ,  C12N15/74 ,  C12N15/90

CPC分类号： C12P7/10 ,  C12N9/22 ,  C12N15/11 ,  C12N15/74 ,  C12N15/902 ,  C12N2310/20 ,  C12N2800/101 ,  C12N2800/80

摘要： A genetically engineered strain of Z. mobilis and uses thereof are provided. The genetically engineered strain named ZMNP is obtained by knockouts of four endogenous plasmids of pZM32, pZM32, pZM36, pZM33, and pZM39 from strain ZM4-Cas12a. ZMNP contains a reduced genome and has a series of excellent traits, such as high transformation efficiency, enhanced tolerance to inhibitors, improved capability to use secondary mother liquor and other excellent performance. ZMNP can be used as a chassis cell to construct diverse cell factories for biochemical production using different feedstocks especially the lignocellulosic biomass.

公开(公告)号：US20230287398A1

公开(公告)日：2023-09-14

申请号：US18315140

申请日：2023-05-10

申请人： Hubei University

发明人： Shihui YANG ,  Yi WANG ,  Yunhao CHEN ,  Yueyue CHEN ,  Qiaoning HE

IPC分类号： C12N15/10 ,  C12N15/74 ,  C12N15/70 ,  C12N1/20

CPC分类号： C12N15/1082 ,  C12N15/74 ,  C12N15/70 ,  C12N1/20

摘要： A construction method of a tight regulation system for gene expression in Zywmmonas mobilis and applications are provided. The T7 expression system in Zymomonas mobilis has been constructed. The T7 expression system is used to construct a tight regulation circuit, help analyze the function of toxic genes and realize the tight regulation of metabolic pathways in Zymomonas mobilis. At the same time, a shuttle plasmids that can be used in Zymomonas mobilis and Escherichia coli., also be constructed to facilitate the construction of plasmid and improve the expression efficiency of foreign genes in Zymomonas mobilis, laying a foundation for subsequent protein expression and secretion and metabolic engineering pathway optimization regulation.