公开(公告)号：US20180298435A1

公开(公告)日：2018-10-18

申请号：US15755957

申请日：2016-07-05

Applicant: Hitachi, Ltd.

Inventor： Junko TANAKA ,  Yoshinobu KOHARA ,  Kunio HARADA ,  Yu ISHIGE

IPC: C12Q1/6869 ,  C12M1/00 ,  G06F19/10 ,  B01J19/00

Abstract: The present invention was made to provide novel methods for detecting DNA and devices therefor. Specifically, the present invention is a method of detecting DNA including the steps of: adding, to an oil, a fluorescently-labeled probe, a DNA intercalator, and a DNA solution containing a target DNA to produce droplets; performing PCR on the droplets; and measuring fluorescence from the fluorescently-labeled probe and fluorescence from the DNA intercalator, wherein the DNA solution has a concentration at which each droplet is produced so as to contain one or less target DNA molecule. In addition, the present invention is a DNA detection device including a droplet production unit, a thermal cycler unit, and a fluorescence detection unit which perform these steps.

公开(公告)号：US20130274126A1

公开(公告)日：2013-10-17

申请号：US13802970

申请日：2013-03-14

Applicant: HITACHI, LTD.

Inventor： Yu ISHIGE ,  Masao KAMAHORI

IPC: C12Q1/04

CPC classification number: C12Q1/04

Abstract: Provided is a device that can detect cells or bacteria in units of a single cell or bacterium, and can further measure the amounts of activity of cells or bacteria or responses of the cells or bacteria to drugs in units of a single cell or bacterium. A plurality of partitioned regions each having about the same size as a cell or a bacterium is provided, and a plurality of types of electrical sensors 201 and 202 are arranged in each partitioned region.

Abstract translation: 提供了可以以单个细胞或细菌为单位检测细胞或细菌的装置，并且可以以单个细胞或细菌为单位进一步测量细胞或细菌的活性或细胞或细菌对药物的反应的量。 提供了与细胞或细菌具有大致相同尺寸的多个分割区域，并且在每个分割区域中布置有多种类型的电传感器201和202。