公开(公告)号：US11359186B2

公开(公告)日：2022-06-14

申请号：US16001031

申请日：2018-06-06

Applicant: Hiroshima University

Inventor： Takashi Yamamoto ,  Tetsushi Sakuma ,  Hiroshi Ochiai ,  Shinya Matsuura ,  Tatsuo Miyamoto

IPC: C12N15/00 ,  C12N9/22 ,  C12N15/63 ,  C40B40/08

Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.

公开(公告)号：US20160186151A1

公开(公告)日：2016-06-30

申请号：US14911154

申请日：2014-05-09

Applicant: HIROSHIMA UNIVERSITY

Inventor： Takashi Yamamoto ,  Tetsushi Sakuma ,  Hiroshi Ochiai ,  Shinya Matsuura ,  Tatsuo Miyamoto

IPC: C12N9/22 ,  C12N15/10

CPC classification number: C12N9/22 ,  C07K2319/81 ,  C12N15/10 ,  C12N15/1093 ,  C12N15/64 ,  C12N15/66 ,  C12Y301/00

Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.

Abstract translation: 本发明提供一种人造核酸酶，其包含通过由35至55个氨基酸残基组成的多肽相互连接的DNA结合结构域和功能结构域，其中DNA-结合模块中两个位点处的氨基酸残基包含在DNA- 结合域表现出每四个DNA结合模块不同的重复模式; 用于表达所述人造核酸酶的载体; 用于制备所述载体的载体文库; 以及用于制备所述载体文库的载体集。

公开(公告)号：US20160152990A1

公开(公告)日：2016-06-02

申请号：US15019630

申请日：2016-02-09

Applicant: HIROSHIMA UNIVERSITY

Inventor： Takashi Yamamoto ,  Tetsushi Sakuma ,  Hiroshi Ochiai ,  Shinya Matsuura ,  Tatsuo Miyamoto

IPC: C12N15/64 ,  C12N9/22 ,  C12N15/10

CPC classification number: C12N9/22 ,  C07K2319/80 ,  C12N15/63 ,  C40B40/08

Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.

公开(公告)号：US20220389396A1

公开(公告)日：2022-12-08

申请号：US17743526

申请日：2022-05-13

Applicant: Hiroshima University

Inventor： Takashi Yamamoto ,  Tetsushi Sakuma ,  Hiroshi Ochiai ,  Shinya Matsuura ,  Tatsuo Miyamoto

IPC: C12N9/22 ,  C12N15/63 ,  C40B40/08

Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.

公开(公告)号：US20180273921A1

公开(公告)日：2018-09-27

申请号：US16001031

申请日：2018-06-06

Applicant: Hiroshima University

Inventor： Takashi Yamamoto ,  Tetsushi Sakuma ,  Hiroshi Ochiai ,  Shinya Matsuura ,  Tatsuo Miyamoto

IPC: C12N9/22 ,  C12N15/63 ,  C40B40/08

CPC classification number: C12N9/22 ,  C07K2319/80 ,  C12N15/63 ,  C40B40/08

Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.

公开(公告)号：US10006011B2

公开(公告)日：2018-06-26

申请号：US15019630

申请日：2016-02-09

Applicant: HIROSHIMA UNIVERSITY

Inventor： Takashi Yamamoto ,  Tetsushi Sakuma ,  Hiroshi Ochiai ,  Shinya Matsuura ,  Tatsuo Miyamoto

IPC: C12N9/22 ,  C12N15/63 ,  C40B40/08

CPC classification number: C12N9/22 ,  C07K2319/80 ,  C12N15/63 ,  C40B40/08

Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.

公开(公告)号：US10030235B2

公开(公告)日：2018-07-24

申请号：US14911154

申请日：2014-05-09

Applicant: HIROSHIMA UNIVERSITY

Inventor： Takashi Yamamoto ,  Tetsushi Sakuma ,  Hiroshi Ochiai ,  Shinya Matsuura ,  Tatsuo Miyamoto

IPC: C12N9/22 ,  C12N15/10 ,  C12N15/64 ,  C12N15/66

Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.