Extraction of neutral lipids by a two solvent method
    2.
    发明授权
    Extraction of neutral lipids by a two solvent method 有权
    通过两种溶剂法提取中性脂质

    公开(公告)号:US09120987B2

    公开(公告)日:2015-09-01

    申请号:US13625483

    申请日:2012-09-24

    Inventor: Aniket Kale

    Abstract: A method for separating neutral lipids from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting neutral lipids from the algal cells. The methods provide for single and multistep extraction processes which allow for efficient separation of algal neutral lipids from a wet algal biomass while avoiding emulsification of extraction mixtures. The neutral lipids are removed after first removing a polar lipid fraction and a protein fraction. These neutral lipids can be used to generate renewable fuels as well as food products and supplements.

    Abstract translation: 使用两亲性溶剂组和疏水性溶剂组从植物材料,特别是完整的藻类细胞中分离中性脂质的方法。 一些实施方案包括将完整的藻细胞脱水,然后从藻类细胞中提取中性脂质。 该方法提供单步和多步提取方法,其允许藻类中性脂质与湿藻类生物质的有效分离,同时避免提取混合物的乳化。 首先除去极性脂质部分和蛋白质级分后,除去中性脂质。 这些中性脂质可用于生产可再生燃料以及食品和补充剂。

    Patterned electrical pulse microorganism aggregation
    3.
    发明授权
    Patterned electrical pulse microorganism aggregation 失效
    图案电脉冲微生物聚集

    公开(公告)号:US08709258B2

    公开(公告)日:2014-04-29

    申请号:US13899813

    申请日:2013-05-22

    CPC classification number: C12N13/00 B03D2203/003 C12M47/02 C12N1/02 C12N1/12

    Abstract: Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may comprise different types of pulses. In some embodiments, the flow path for the aqueous suspension may vary.

    Abstract translation: 本文描述了用于在水性悬浮液中聚集微生物的系统,方法和装置。 特别地,当水悬浮液遵循流动路径以引起微生物聚集时,将电场施加到包含微生物的水性悬浮液中的系统,方法和装置。 电场可以包括不同类型的脉冲。 在一些实施方案中,水性悬浮液的流动路径可以变化。

    Electrical microorganism aggregation methods
    4.
    发明授权
    Electrical microorganism aggregation methods 失效
    电微生物聚集方法

    公开(公告)号:US08702991B2

    公开(公告)日:2014-04-22

    申请号:US13899769

    申请日:2013-05-22

    CPC classification number: C12N13/00 B03D2203/003 C12M47/02 C12N1/02

    Abstract: Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may be continuous or pulsed. In some embodiments, the flow path for the aqueous suspension may vary.

    Abstract translation: 本文描述了用于在水性悬浮液中聚集微生物的系统,方法和装置。 特别地,当水悬浮液遵循流动路径以引起微生物聚集时,将电场施加到包含微生物的水性悬浮液中的系统,方法和装置。 电场可以是连续的或脉冲的。 在一些实施方案中,水性悬浮液的流动路径可以变化。

    Rectangular channel electro-acoustic aggregation device
    5.
    发明授权
    Rectangular channel electro-acoustic aggregation device 失效
    矩形通道电声聚合装置

    公开(公告)号:US08668827B2

    公开(公告)日:2014-03-11

    申请号:US13899873

    申请日:2013-05-22

    CPC classification number: C12N13/00 C12M33/12 C12M47/02 C12N1/04 C12N1/12

    Abstract: Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field and/or acoustic energy to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may be continuous or pulsed. In some embodiments, the flow path for the aqueous suspension may vary.

    Abstract translation: 本文描述了用于在水性悬浮液中聚集微生物的系统,方法和装置。 特别地,是当包含微生物的水性悬浮液伴随着流动路径引起微生物聚集时,将电场和/或声能施加到水性悬浮液的系统,方法和装置。 电场可以是连续的或脉冲的。 在一些实施方案中,水性悬浮液的流动路径可以变化。

    Tubular electro-acoustic aggregation device
    6.
    发明授权
    Tubular electro-acoustic aggregation device 失效
    管状电声聚合装置

    公开(公告)号:US08709250B2

    公开(公告)日:2014-04-29

    申请号:US13899840

    申请日:2013-05-22

    CPC classification number: C12N13/00 C12M33/12 C12M47/02 C12N1/04 C12N1/12

    Abstract: Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field and/or acoustic energy to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may be continuous or pulsed. In some embodiments, the flow path for the aqueous suspension may vary.

    Abstract translation: 本文描述了用于在水性悬浮液中聚集微生物的系统,方法和装置。 特别地,是当包含微生物的水性悬浮液伴随着流动路径引起微生物聚集时,将电场和/或声能施加到水性悬浮液的系统,方法和装置。 电场可以是连续的或脉冲的。 在一些实施方案中,水性悬浮液的流动路径可以变化。

    Tunable electrical field for aggregating microorganisms
    7.
    发明授权
    Tunable electrical field for aggregating microorganisms 失效
    用于聚集微生物的可调电场

    公开(公告)号:US08673154B2

    公开(公告)日:2014-03-18

    申请号:US13899902

    申请日:2013-05-22

    CPC classification number: C12N13/00 B03D2203/003 C12M47/02 C12N1/02

    Abstract: Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may be continuous or pulsed. In some embodiments, the flow path for the aqueous suspension may vary. In some embodiments, the cross-sectional area of the electrical field may be tuned.

    Abstract translation: 本文描述了用于在水性悬浮液中聚集微生物的系统,方法和装置。 特别地,当水悬浮液遵循流动路径以引起微生物聚集时,将电场施加到包含微生物的水性悬浮液中的系统,方法和装置。 电场可以是连续的或脉冲的。 在一些实施方案中,水性悬浮液的流动路径可以变化。 在一些实施例中,可以调整电场的横截面积。

    Extraction of proteins by a two solvent method
    8.
    发明授权
    Extraction of proteins by a two solvent method 失效
    通过两种溶剂法提取蛋白质

    公开(公告)号:US08551336B2

    公开(公告)日:2013-10-08

    申请号:US13674631

    申请日:2012-11-12

    Inventor: Aniket Kale

    CPC classification number: C11B1/10 C11B1/108 C11C3/003 C12N1/06

    Abstract: A method for separating proteins from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting proteins from the algal cells. The methods provide for single and multistep extraction processes which allow for efficient separation of algal proteins from a wet algal biomass. These proteins are high value products which can be used as renewable sources of food and food additives. Neutral lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.

    Abstract translation: 使用两亲性溶剂组和疏水性溶剂组从植物材料,特别是完整藻细胞中分离蛋白质的方法。 一些实施方案包括将完整的藻细胞脱水,然后从藻细胞中提取蛋白质。 该方法提供了单步和多步提取方法,其允许藻类蛋白质与湿藻类生物质的有效分离。 这些蛋白质是可以用作食品和食品添加剂的可再生资源的高价值产品。 提取蛋白质后残留在藻类生物质中的中性脂质可用于产生可再生燃料。

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