公开(公告)号：US11181502B2

公开(公告)日：2021-11-23

申请号：US15129854

申请日：2015-03-26

Applicant: Hitachi High-Technologies Corporation

Inventor： Naoshi Itabashi ,  Sonoko Migitaka ,  Itaru Yanagi ,  Rena Akahori ,  Kenichi Takeda

IPC: G01N21/63 ,  G01N27/00 ,  G01N27/92 ,  G01N27/447 ,  G01N21/64 ,  G01N21/65 ,  G01N33/487 ,  B82Y40/00

Abstract: While an insulating film having a near-field light generating element placed thereon is being irradiated with light in an electrolytic solution, or after the film that has been irradiated with light is disposed in the electrolytic solution, a first voltage is applied between the two electrodes installed in the electrolytic solution across the film, a second voltage is then applied between the two electrodes, and a value of a current that flows between the two electrodes due to the application of the second voltage is detected. This procedure is stopped when the current value reaches or exceeds a pre-set threshold value, whereby a hole is formed at a desired location in the thin-film.

公开(公告)号：US11169139B2

公开(公告)日：2021-11-09

申请号：US16395708

申请日：2019-04-26

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Rena Akahori ,  Itaru Yanagi ,  Kenichi Takeda

IPC: G01N33/487 ,  G01N33/543 ,  C12Q1/6869 ,  G01N27/447

Abstract: To introduce a biomolecule into a nanopore without the need to check the position of the nanopore in a thin film. In addition, displacement stability is ensured and stable acquisition of blocking signals is realized. An immobilization member 107 having a larger size than a thin film 113 with a nanopore 112 is used, and biomolecules are immobilized on the biomolecule immobilization member 107 at a density that allows at least one biomolecule 108 to enter an electric field region around the nanopore when the biomolecule immobilization member 107 has moved close to a nanopore device 101.

公开(公告)号：US20150308977A1

公开(公告)日：2015-10-29

申请号：US14418480

申请日：2013-06-28

Applicant: Hitachi High-Technologies Corporation

Inventor： Toshiro Saito ,  Kenta Imai ,  Kyoko Imai ,  Kazumichi Imai ,  Itaru Yanagi ,  Yoshimitsu Yanagawa ,  Masahiko Ando ,  Naoshi Itabashi

IPC: G01N27/414 ,  G01N33/543

CPC classification number: G01N27/4145 ,  G01N27/4148 ,  G01N33/48721 ,  G01N33/543 ,  G01N33/54306 ,  G01N37/00

Abstract: The present invention is intended to provide a method and a device for detecting a biomolecule with high sensitivity and high throughput over a wide dynamic range without requiring concentration adjustments of a sample in advance. The present invention specifically binds charge carriers to a detection target biomolecule, and detects the detection target biomolecule one by one by measuring a current change that occurs as the conjugate of the biomolecule and the charge carriers passes through a micropore. High-throughput detection of a biomolecule sample is possible with an array of detectors.

Abstract translation: 本发明旨在提供一种用于在宽动态范围内检测具有高灵敏度和高产量的生物分子的方法和装置，而不需要预先对样品进行浓度调节。 本发明将电荷载体特异性地结合到检测目标生物分子，并且通过测量随着生物分子和电荷载体的共轭体通过微孔而发生的电流变化逐个检测检测目标生物分子。 生物分子样品的高通量检测是可能的与一组检测器。

公开(公告)号：US10753922B2

公开(公告)日：2020-08-25

申请号：US15779931

申请日：2016-11-29

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Rena Akahori ,  Kenichi Takeda ,  Itaru Yanagi

IPC: G01N33/487 ,  G01N27/327 ,  G01N27/414 ,  B01L3/00

Abstract: A biomolecule measuring device includes a first liquid tank and a second liquid tank which are filled with an electrolytic solution, a nanopore device that supports a thin film having a nanopore and is provided between the first liquid tank and the second liquid tank so as to communicate between the first liquid, tank and the second liquid tank through the nanopores, and an immobilizing member that is disposed in the first liquid tank, has a size larger than that of the thin film, and to which the biomolecules are immobilized, in which at least, one of the nanopore device and the immobilizing member has a groove structure.

公开(公告)号：US10294525B2

公开(公告)日：2019-05-21

申请号：US15531500

申请日：2015-10-28

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Rena Akahori ,  Itaru Yanagi ,  Kenichi Takeda

IPC: C12Q1/6869 ,  G01N27/447 ,  G01N33/487

Abstract: To introduce a biomolecule into a nanopore without the need to check the position of the nanopore in a thin film. In addition, displacement stability is ensured and stable acquisition of blocking signals is realized. An immobilization member 107 having a larger size than a thin film 113 with a nanopore 112 is used, and biomolecules are immobilized on the biomolecule immobilization member 107 at a density that allows at least one biomolecule 108 to enter an electric field region around the nanopore when the biomolecule immobilization member 107 has moved close to a nanopore device 101.

公开(公告)号：US11499959B2

公开(公告)日：2022-11-15

申请号：US16463502

申请日：2016-12-09

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Yoshimitsu Yanagawa ,  Kenichi Takeda ,  Itaru Yanagi ,  Yusuke Goto ,  Kazuma Matsui

IPC: G01N33/487 ,  B01J19/08 ,  C25F3/12 ,  B81C1/00 ,  G01N33/00 ,  B23H7/20

Abstract: A first modulation voltage is applied to a thin film. An amount of a change in the phase of a current carried through the thin film with respect to the phase of the first modulation voltage is compared with a threshold. Upon detecting that the amount of the change in the phase exceeds the threshold is detected, the application of the first modulation voltage is stopped. Thus, a nanopore is formed on the thin film at high speed.

公开(公告)号：US09759681B2

公开(公告)日：2017-09-12

申请号：US14418480

申请日：2013-06-28

Applicant: Hitachi High-Technologies Corporation

Inventor： Toshiro Saito ,  Kenta Imai ,  Kyoko Imai ,  Kazumichi Imai ,  Itaru Yanagi ,  Yoshimitsu Yanagawa ,  Masahiko Ando ,  Naoshi Itabashi

IPC: G01N27/414 ,  G01N33/487 ,  G01N33/543 ,  G01N37/00

CPC classification number: G01N27/4145 ,  G01N27/4148 ,  G01N33/48721 ,  G01N33/543 ,  G01N33/54306 ,  G01N37/00

Abstract: The present invention is intended to provide a method and a device for detecting a biomolecule with high sensitivity and high throughput over a wide dynamic range without requiring concentration adjustments of a sample in advance. The present invention specifically binds charge carriers to a detection target biomolecule, and detects the detection target biomolecule one by one by measuring a current change that occurs as the conjugate of the biomolecule and the charge carriers passes through a micropore. High-throughput detection of a biomolecule sample is possible with an array of detectors.