公开(公告)号：US20220187240A1

公开(公告)日：2022-06-16

申请号：US17547377

申请日：2021-12-10

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Yuji KIMURA ,  Kazuya SUZUKI ,  Ryo MAKINO ,  Hiroyasu ITOH

IPC: G01N27/447 ,  G01N21/64 ,  B03C5/00 ,  B03C5/02

Abstract: A microparticle trapping device includes: a fluid channel configured to be injected with a fluid including a microparticle; first and second electrodes configured to generate an electric field in the fluid channel; and an electrical insulator formed with at least one opening between the first and second electrodes in the fluid channel. The electrical insulator is disposed between the first and second electrodes so that an inhomogeneous electric field is made through the at least one opening between the first and second electrodes in the fluid channel, and the still other aspect is configured to trap the microparticle through dielectrophoresis.

公开(公告)号：US20220120271A1

公开(公告)日：2022-04-21

申请号：US17310814

申请日：2020-02-19

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Yuji KIMURA ,  Hiroyasu ITOH

IPC: F04B53/16 ,  F04B53/10 ,  F04B43/04 ,  C12M1/00

Abstract: In a liquid supply device, the pump unit has a discharge port for discharging a fluid. The connecting portion is connected to the discharge port. The first and second pipe portions and branch off from the connecting portion. The first introduction unit is connected to the first pipe portion and introduces a first liquid into the first flow channel at a flow rate corresponding to a flow rate of the fluid flowing through the first pipe portion. The second introduction unit is connected to the second pipe portion and introduces a second liquid into the first flow channel at a flow rate corresponding to a flow rate of the fluid flowing through the second pipe portion. The stopping portion stops the flow of the fluid in the second pipe portion.

公开(公告)号：US20220155297A1

公开(公告)日：2022-05-19

申请号：US17423133

申请日：2019-11-06

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Yuji KIMURA ,  Sayaka KAZAMI ,  Yu HASHIMOTO ,  Hiroyasu ITOH

IPC: G01N33/566 ,  G01N33/533 ,  G01N21/78 ,  C12M1/40

Abstract: A method for selecting cells highly responsive to a target substance according to the present invention comprises step of: (1a) bringing a sample containing a target substance into contact with a plurality of cells, wherein the cells are cells having a receptor for the target substance and a fluorescent indicator, and the fluorescent indicator emits fluorescence as a result of binding between the target substance and the receptor; (1b) calculating a fluorescence intensity increase rate for each of the cells; and (1c) selecting arbitrary cells exhibiting a fluorescence intensity increase rate within the top 50% among the plurality of cells. According to the present invention, it is possible to detect the target substance in the sample with high sensitivity.

公开(公告)号：US20160247291A1

公开(公告)日：2016-08-25

申请号：US15031802

申请日：2014-10-02

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Tomoko OTSU ,  Taro ANDO ,  Hiroyasu ITOH ,  Haruyoshi TOYODA ,  Yoshiyuki OHTAKE ,  Yuu TAKIGUCHI

IPC: G06T7/20 ,  G21K1/00 ,  G02F1/13 ,  H04N5/232 ,  G02B7/04 ,  G02B27/09

CPC classification number: G06T7/248 ,  B01L3/502761 ,  B01L2300/0822 ,  B01L2400/0454 ,  G02B7/04 ,  G02B21/32 ,  G02B27/0927 ,  G02F1/1313 ,  G06T2207/10148 ,  G06T2207/10152 ,  G06T2207/30241 ,  G21K1/006 ,  H04N5/23212

Abstract: A micro object control apparatus for controlling motion of a micro object within a medium includes a light source, an optical vortex generation unit, an objective lens, an imaging unit, an analysis unit, and a movement unit. The analysis unit acquires first motion information of the micro object based on the image data in which the micro object optically trapped with the optical vortex is imaged by setting the focal position of the optical vortex to a first position, acquires second motion information of the micro object based on the image data in which the micro object optically trapped with the optical vortex is imaged by setting the focal position of the optical vortex to a second position, and evaluates a state of an optical trap of the micro object with the optical vortex by comparing the first motion information and the second motion information.

Abstract translation: 用于控制介质内的微物体的运动的微物体控制装置包括光源，光漩涡产生单元，物镜，成像单元，分析单元和移动单元。 分析单元基于通过将光学旋涡的焦点位置设置为第一位置来成像光学捕获的微物体的图像数据来获取微物体的第一运动信息，获取微型物体的第二运动信息 基于通过将光学旋涡的焦点位置设置到第二位置来成像光学旋涡被光学捕获的微物体的图像数据的对象，并且利用光学涡流评估微物体的光阱的状态 比较第一运动信息和第二运动信息。