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公开(公告)号：US11447540B2
公开(公告)日：2022-09-20
申请号：US16169130
申请日：2018-10-24
Applicant: H. LUNDBECK A/S
Inventor： Gary Lesnicki , Patricia Dianne McNeill , Franz Hartner , Mark Young
Abstract: Methods for producing heterologous proteins are disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.
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2.发明授权公开(公告)号：US12077794B2
公开(公告)日：2024-09-03
申请号：US16272079
申请日：2019-02-11
Applicant: H. LUNDBECK A/S
Inventor： Gary Lesnicki , Mark Young
CPC classification number: C12P21/00 , C12N1/16 , C12N15/67 , C12N15/905 , C07K2317/14 , C12N2523/00
Abstract: Methods for producing heterologous proteins are disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.
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公开(公告)号：US11597758B2
公开(公告)日：2023-03-07
申请号：US16258014
申请日：2019-01-25
Applicant: H. LUNDBECK A/S
Inventor： Gary Lesnicki , Patricia Dianne McNeill , Franz Hartner , Mark Young
Abstract: A feedback control mechanism for a fermentation of yeast cells to make recombinant proteins uses a respiratory quotient measurement which adjusts the levels of oxygenation and/or fermentable sugar feed. The feedback control mechanism permits well controlled cultures that produce good amounts of product while avoiding toxic accumulation of ethanol. Additionally, recombinant proteins so produced have excellent qualitative properties, such as excellent homogeneity and proper inter-subunit assembly.
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