公开(公告)号：US20140045251A1

公开(公告)日：2014-02-13

申请号：US14060650

申请日：2013-10-23

Applicant: General Electric Company

Inventor： Anup Sood ,  Michael Christopher Montalto ,  Michael John Gerdes ,  Maximilian Lewis Seel ,  Robert John Filkins

IPC: G01N33/53

CPC classification number: G01N33/53 ,  C12Q1/68 ,  G01N33/542 ,  G01N33/582 ,  Y10T436/143333

Abstract: Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.

Abstract translation: 提供了用于检测生物样品中多个靶的方法。 所述方法包括使样品与第一探针接触; 将第一探针物理地绑定到第一靶; 观察来自第一探针的第一信号; 施用化学试剂来修饰第一信号; 使样品与第二探针接触; 将第二探针物理地绑定到第二靶上; 并观察来自第二探测器的第二信号。 本文公开的方法还提供绑定，观察，信号修改的多次迭代，用于导出关于单个样本中的多个目标的信息。 还提供了相关的套件和设备。