公开(公告)号：US20240131510A1

公开(公告)日：2024-04-25

申请号：US18235564

申请日：2023-08-17

Applicant: GEORGE MASON UNIVERSITY

Inventor： Remi VENEZIANO ,  Ramin M. HAKAMI ,  Hunter MASON

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0861

Abstract: A microfluidic intercellular communication analysis device includes a coverslip and a Polydimethylsiloxane (PDMS) layer attached to the coverslip, the PDMS layer comprising a plurality of microfluidic channels each having an inlet and an outlet, the plurality of microfluidic channels comprising a donor cell channel structured to receive a donor cell population, a recipient cell channel structured to receive at least a recipient cell population and a matrix channel comprising a diffusion barrier having pores, the donor cell channel and the recipient cell channel each comprising inlets and outlets having an arc angle ranging from 180° to 300°, the arc angle structured to prevent cell aggregation in the inlets, the outlets and/or channel surfaces thereof, wherein upon injecting the donor cell population and the recipient cell population, the target subject is imaged by an imaging device and analyzed for intercellular communication and/or functional characterizations for ensuing intercellular communication effects.

公开(公告)号：US20240226881A9

公开(公告)日：2024-07-11

申请号：US18235564

申请日：2023-08-18

Applicant: GEORGE MASON UNIVERSITY

Inventor： Remi VENEZIANO ,  Ramin M. HAKAMI ,  Hunter MASON

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0861

Abstract: A microfluidic intercellular communication analysis device includes a coverslip and a Polydimethylsiloxane (PDMS) layer attached to the coverslip, the PDMS layer comprising a plurality of microfluidic channels each having an inlet and an outlet, the plurality of microfluidic channels comprising a donor cell channel structured to receive a donor cell population, a recipient cell channel structured to receive at least a recipient cell population and a matrix channel comprising a diffusion barrier having pores, the donor cell channel and the recipient cell channel each comprising inlets and outlets having an arc angle ranging from 180° to 300°, the arc angle structured to prevent cell aggregation in the inlets, the outlets and/or channel surfaces thereof, wherein upon injecting the donor cell population and the recipient cell population, the target subject is imaged by an imaging device and analyzed for intercellular communication and/or functional characterizations for ensuing intercellular communication effects.