公开(公告)号：US20030157523A1

公开(公告)日：2003-08-21

申请号：US10300546

申请日：2002-11-20

Applicant: GENENTECH, INC.

Inventor： Gretchen Frantz ,  Trent Harris Landon ,  Franklin V. Peale, JR. ,  Thinh Quang Pham ,  Jean Philippe F. Stephan ,  Debra Y. Dunlap ,  Kenneth J. Hillan

IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/567 ,  G01N033/574 ,  B28B001/48 ,  B29D019/08 ,  C12M001/34

CPC classification number: G01N1/36 ,  B01J2219/00283 ,  B01J2219/00286 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00495 ,  B01J2219/00659 ,  B01J2219/00673 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01L3/5085 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/554 ,  G01N33/57438 ,  G01N2001/368

Abstract: The invention relates to biological arrays, biological microarrays, and methods of using the arrrays and microarrays to detect the amount and/or presence of a biological molecule in a biological sample. Biological arrays of the invention comprise a solidified, sectionable matrix comprising a plurality of wells disposed therein and one or more biological samples disposed within the plurality of wells, which biological arrays optionally comprise an internal standard preparation and/or an orientation marker. Sections or slices of the biological arrays are mounted on a planar substrate surface to form cellular microarrays of the invention. In alternative cellular microarrays of the invention, the matrix material is a temperature-sensitive material removable from the microarray leaving cellular biological material on the substrate surface.

Abstract translation: 本发明涉及生物阵列，生物微阵列，以及使用这些阵列和微阵列来检测生物样品中生物分子的量和/或存在的方法。 本发明的生物阵列包括固化的可切割基质，其包括设置在其中的多个孔和设置在所述多个孔内的一个或多个生物样品，所述生物阵列任选地包含内标制剂和/或取向标记物。 将生物阵列的切片或切片安装在平坦的基底表面上以形成本发明的细胞微阵列。 在本发明的替代细胞微阵列中，基质材料是可从微阵列移除的温度敏感材料，在基底表面上留下细胞生物材料。