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公开(公告)号：US11776801B2
公开(公告)日：2023-10-03
申请号：US17470769
申请日：2021-09-09
Applicant: Fluidigm Canada Inc.
Inventor： Alexander Loboda , Raymond Jong , Michael Sullivan , Serguei Vorobiev , Robert Rotenberg , Emil D. Stratulativ , Maxim Voronov , Mark Armstrong
CPC classification number: H01J49/105 , H01J49/0027 , H01J49/0463 , H05H1/2406 , H05H1/30
Abstract: Inductively coupled plasma (ICP) analyzers use an ICP torch to generate a plasma in which a sample is atomized an ionized. Analysis of the atomic ions can be performed by atomic analysis, such as mass spectrometry (MS) or atomic emission spectrometry (AES). Particle based ICP analysis includes analysis of particles such as cells, beads, or laser ablation plumes, by atomizing and ionizing particles in an ICP torch followed by atomic analysis. In mass cytometry, mass tags of particles are analyzed by mass spectrometry, such as by ICP-MS. Systems and methods of the subject application include one or more of: a demountable ICP torch holder assembly, an external ignition device; an ICP load coil comprising an annular fin, particle suspension sample introduction fluidics, and ICP analyzers thereof.
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公开(公告)号：US20230013375A1
公开(公告)日：2023-01-19
申请号：US17756475
申请日：2020-11-25
Applicant: FLUIDIGM CANADA INC.
Inventor： Daaf Sandkuijl , Alexander Loboda , Vincent Yi-Ching Yeh , Ladan Gheiratmand
Abstract: Methods and systems for automated slide handling for imaging applications are described herein. In certain aspects, an automated slide handler may be operatively coupled to a slide hotel and/or one or more imaging systems described herein. The automated slide handler may be a robotic arm with up to 6 degrees of freedom. Automated slide handling may include sample preparation, such as sectioning and staining. Suitable imaging systems include a fluorescence microscope or an imaging mass cytometer. Methods and systems disclosed herein enable high throughput profiling of tissue sections.
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公开(公告)号：US12013391B2
公开(公告)日：2024-06-18
申请号：US16383241
申请日：2019-04-12
Applicant: Fluidigm Canada Inc.
Inventor： Vladimir Baranov , Alexander Loboda , Michael Sullivan
CPC classification number: G01N33/5306 , G01N33/58 , H01J49/105 , G01N2458/15 , G01N2560/00
Abstract: Analyzing samples injected into an inductively coupled plasma source can be improved by one or more of a stabilizing solution mixable with a sample prior to injection and a heated injector. The stabilizing solution can minimize the difference in osmotic pressure between the solution and the cells with a relatively low amount of dissolved solids (e.g., at or below about 0.2%). The stabilizing solution can contain a salt (e.g., ammonium nitrate) present in concentrations of at least 5 mM. The injector can be heated before and/or during injection. In some cases, heat from adjacent parts can be channeled into the injector to improve heating of the injector. An injector heated to sufficient temperatures can minimize solute buildup and can extend the usable time between cleanings. These improvements can be especially useful in elemental analysis, such as inductively coupled plasma mass spectrometry or inductively coupled plasma optical emission spectrometry.
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Patent Agency Ranking
公开(公告)号：US20220310373A1
公开(公告)日：2022-09-29
申请号：US17620049
申请日：2020-06-17
Applicant: Fluidigm Canada Inc.
Inventor： Paul Corkum , Alexander Loboda , David M. Rayner
Abstract: Embodiments of the present invention relate to replacement of the previous ICP-based ionisation system with a new laser ionisation system, providing improved mass spectrometer-based apparatus and methods for using them to analyse samples, in particular the use of mass spectrometry mass cytometry, imaging mass spectrometry and imaging mass cytometry, for the analysis of biological samples. Accordingly, embodiments of the present invention provide an apparatus, for example a mass cytometer, comprising: 1) a sampler; 2) a laser ionisation system to receive material removed from the sample by the sampler, wherein the laser ionisation system comprises an ionisation system conduit and a pulsed laser adapted to ionise sample material passing through or exiting the ionisation system conduit; and 3) a mass spectrometer to receive elemental ions from said ionisation system and to analyse said elemental ions.
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公开(公告)号：US20220093382A1
公开(公告)日：2022-03-24
申请号：US17470769
申请日：2021-09-09
Applicant: Fluidigm Canada Inc.
Inventor： Alexander Loboda , Raymond Jong , Michael Sullivan , Serguei Vorobiev , Robert Rotenberg , Emil D. Stratulativ , Maxim Voronov , Mark Armstrong
Abstract: Inductively coupled plasma (ICP) analyzers use an ICP torch to generate a plasma in which a sample is atomized an ionized. Analysis of the atomic ions can be performed by atomic analysis, such as mass spectrometry (MS) or atomic emission spectrometry (AES). Particle based ICP analysis includes analysis of particles such as cells, beads, or laser ablation plumes, by atomizing and ionizing particles in an ICP torch followed by atomic analysis. In mass cytometry, mass tags of particles are analyzed by mass spectrometry, such as by ICP-MS. Systems and methods of the subject application include one or more of: a demountable ICP torch holder assembly, an external ignition device; an ICP load coil comprising an annular fin, particle suspension sample introduction fluidics, and ICP analyzers thereof.
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公开(公告)号：US20220155308A1
公开(公告)日：2022-05-19
申请号：US17439503
申请日：2020-03-27
Applicant: Fluidigm Canada Inc.
Inventor： Stephen K.H. Li , Daniel Majonis , Vladimir Baranov , Olga Ornatsky , Bedilu Allo , Dmitry Bandura , Colin Thom , Greg Stelzer , Alexander Loboda
Abstract: A lyophilized antibody panel is disclosed for interrogation using elemental analysis. The antibody panel includes multiple antibodies each element-tagged or element-labelled with one or more isotopes such that each different antibody is isotopically distinguishable from the other antibodies. Each element tag can include one or more unique isotopes or unique combinations of isotopes. The set of element-tagged antibodies can be lyophilized in admixture. Thus, the lyophilized element-tagged antibody panel can be easily and efficiently resuspended and mixed with a sample prior to interrogation with an elemental analyzer, such as a mass spectrometer. This lyophilized element-tagged antibody panel can provide the benefits of an element-tagged assay while also being easy to use and remaining stable for long durations.
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公开(公告)号：US20210391161A1
公开(公告)日：2021-12-16
申请号：US17422578
申请日：2020-01-14
Applicant: Fluidigm Canada Inc.
Inventor： Alexander Loboda , Daaf Sandkuijl , Adam Carew
Abstract: As described herein, one or more parameters of a direct ionization imaging mass spectrometer (IMS) may be set to obtain a desired plasma and deliver it to a mass detector. Depending on the application, certain parameters may be predetermined (e.g., a spot size given a desired resolution) and, as described herein, other parameters can be adjusted to obtain the desired plasma properties. Also included is sample preparation suitable for direct ionization IMS and/or other imaging modalities.
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公开(公告)号：US20190317082A1
公开(公告)日：2019-10-17
申请号：US16383241
申请日：2019-04-12
Applicant: Fluidigm Canada Inc.
Inventor： Vladimir Baranov , Alexander Loboda , Michael Sullivan
Abstract: Analyzing samples injected into an inductively coupled plasma source can be improved by one or more of a stabilizing solution mixable with a sample prior to injection and a heated injector. The stabilizing solution can minimize the difference in osmotic pressure between the solution and the cells with a relatively low amount of dissolved solids (e.g., at or below about 0.2%). The stabilizing solution can contain a salt (e.g., ammonium nitrate) present in concentrations of at least 5 mM. The injector can be heated before and/or during injection. In some cases, heat from adjacent parts can be channeled into the injector to improve heating of the injector. An injector heated to sufficient temperatures can minimize solute buildup and can extend the usable time between cleanings. These improvements can be especially useful in elemental analysis, such as inductively coupled plasma mass spectrometry or inductively coupled plasma optical emission spectrometry.
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