公开(公告)号：US20170175068A1

公开(公告)日：2017-06-22

申请号：US15502285

申请日：2015-08-20

Applicant: Eppendorf AG

Inventor： Wilhelm Pluester ,  Jose Remacle ,  Isabelle Alexandre ,  Christelle Plennevaux ,  Francoise De Longueville

IPC: C12M1/00 ,  C12M1/12 ,  G01N33/543

CPC classification number: C12M23/20 ,  C12M25/00 ,  G01N33/54393

Abstract: The present invention relates to a method for coating a solid support which is suitable for being used in cell culture applications. The method comprises a step in which a solution of a carrier protein, which comprises attached thereto or as a part of its amino acid sequence at least one cell function- modulating peptide sequence, is heated to at least 50° C. and incubated same under conditions which result in the formation of soluble carrier protein aggregates comprising between 2 and 1000 protein molecules. Subsequently, the protein aggregates are brought into contact with the solid support under conditions that allow for the non-covalent adsorption of the aggregates to the support. The invention also relates to a coated solid support having non-covalently adsorbed on its surface at least one of the above-mentioned aggregate of carrier proteins aggregates.