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    METHOD
    3.
    发明申请
    METHOD 审中-公开

    公开(公告)号：US20180295858A1

    公开(公告)日：2018-10-18

    申请号：US15520140

    申请日：2015-10-23

    Applicant: DUPONT NUTRITION BIOSCIENCES APS

    Inventor： Svend Haaning ,  Karsten Matthias Kragh ,  Stepan Shipovskov ,  Andrei Miasnikov ,  Maria Ma ,  Luis Fernando Romero Millan ,  Luke Barnard ,  Shukun Yu ,  Ernst Meinjohanns ,  Steffen Bak ,  Päivi Nurminen ,  Heli Putaala

    IPC: A23K10/14 ,  C12N9/48 ,  A23K20/147 ,  A23K50/75 ,  A23K50/30

    Abstract: A method of preparing a feed additive composition comprising: (a) admixing at least one proline tolerant tripeptidyl peptidase predominantly having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having (i) (A) Proline at P1; and (B) An amino acid selected from alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tryptophan, tyrosine, valine or synthetic amino acids at P1; or (ii) (a′) Proline at P1′; and (b′) An amino acid selected from alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tryptophan, tyrosine, valine or synthetic amino acids or amines at P1′; and one or more ingredients selected from the group consisting of a salt, polyol including sorbitol and glycerol, wheat or a wheat component, sodium acetate, sodium acetate trihydrate, potassium sorbate Talc, polyvinyl alcohol (PVA), benzoate, sorbiate, 1,3-propane diol, glucose, parabens, sodium chloride, citrate, metabisulfite, formate or a combination thereof; and (b) optionally packaging as well as uses of such proline tolerant tripeptidyl peptidases, feed additive compositions, feed additive kits, feeds or feedstuffs and/or premixes.

    METHOD
    5.
    发明申请
    METHOD 审中-公开
    方法

    公开(公告)号：US20150152153A1

    公开(公告)日：2015-06-04

    申请号：US14415564

    申请日：2013-07-19

    Applicant: DUPONT NUTRITION BIOSCIENCES APS

    Inventor： Shukun Yu ,  Charlotte Horsmans Poulsen ,  Jon Gade Hansted

    IPC: C07K14/47 ,  A23K1/16 ,  C12P21/06

    CPC classification number: C07K14/4741 ,  A23K10/26 ,  A23K20/147 ,  A23K50/30 ,  A23K50/40 ,  A23K50/75 ,  C12P13/04 ,  C12P21/06

    Abstract: The present invention relates to a method of producing keratin hydrolysate comprising the steps of: i) reacting keratin material with a protease; and ii) reacting keratin material with a chemical oxidant; wherein step ii occurs: a) after step i); b) during step i) when the selected protease hydrolyses under the pH conditions used for the chemical reaction and/or c) prior to step i) when the selected protease hydrolyses under the reaction conditions used for the chemical reaction; keratin hydrolysate so produced and uses thereof.

    Abstract translation: 本发明涉及一种生产角蛋白水解产物的方法，包括以下步骤：i）使角蛋白材料与蛋白酶反应; 和ii）使角蛋白材料与化学氧化剂反应; 其中步骤ii发生：a）在步骤i）之后; b）在步骤i）当选择的蛋白酶在用于化学反应的pH条件下和/或c）之前在步骤i）之前水解，当选择的蛋白酶在用于化学反应的反应条件下水解时; 如此生产的角蛋白水解产物及其用途。

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