公开(公告)号：US09725702B2

公开(公告)日：2017-08-08

申请号：US14427192

申请日：2013-09-11

申请人： DANMARKS TEKNISKE UNIVERSITET

发明人： Anna Sitarz ,  Jørn Dalgaard Mikkelsen ,  Anne Meyer ,  Mateusz Lezyk

IPC分类号： C12N9/24 ,  C12N9/02 ,  C12N9/42 ,  C12P19/14 ,  C12P19/00 ,  C12P7/08 ,  C07H21/04 ,  C12P19/02

CPC分类号： C12N9/0061 ,  C12N9/2437 ,  C12N9/2445 ,  C12P19/02 ,  C12P19/14

摘要： The invention addresses the need for enzymes that can enhance the yield of fermentable sugar from the hydrolysis of lignocellulose biomass, for example sugar cane bagasse, barley straw and wheat straw, such that the use of this biomass can become economically viable. The invention provides methods for the hydrolysis of biomass using a laccase derived from Ganoderma lucidum. Further, the invention provides an enzyme composition comprising a laccase derived from Ganoderma lucidum which may be combined with one or more cellulases, and for its use in enhancing lignocellulose biomass hydrolysis.

公开(公告)号：US10081801B2

公开(公告)日：2018-09-25

申请号：US14783967

申请日：2014-04-11

申请人： DANMARKS TEKNISKE UNIVERSITET

发明人： Jørn Dalgaard Mikkelsen ,  Carsten Jers ,  Malwina Michalak ,  Kasper Planeta Kepp ,  Dorte Møller Larsen

IPC分类号： C12N9/24 ,  A61K31/70 ,  A61K31/7016 ,  A61K31/702 ,  A23L33/00 ,  A23L33/10

CPC分类号： C12N9/2402 ,  A23L33/10 ,  A23L33/40 ,  A23V2002/00 ,  A61K31/7016 ,  A61K31/702 ,  C12Y302/01018 ,  Y02A50/414 ,  Y02P20/582

摘要： The invention provides a mutant enzyme having trans-sialidase activity (EC 3.2.1.18), characterized by an enhanced trans-sialidase:sialidase ratio when compared to its parent sialidase enzyme. Further the enzyme may be used in a method for trans-sialylating mono- and oligo-saccharides, including galacto-oligosaccharides (GOS), fructo-oligosaccharides (FOS), malto-oligosaccharides (MOS), isomalto-oligosaccarides (IMO), lactulose, melibiose, maltose, glycosyl sucrose, lactosucrose and fucose. Trans-sialidated mono- and oligo-saccharides, produced with the mutant enzyme, are useful in preparing infant formula, a prebiotic nutritional supplement, and a food supplement.

公开(公告)号：US20150232815A1

公开(公告)日：2015-08-20

申请号：US14427192

申请日：2013-09-11

申请人： DANMARKS TEKNISKE UNIVERSITET

发明人： Anna Sitarz ,  Jørn Dalgaard Mikkelsen ,  Anne Meyer ,  Mateusz Lezyk

IPC分类号： C12N9/02 ,  C12P19/14 ,  C12P19/02 ,  C12N9/42

CPC分类号： C12N9/0061 ,  C12N9/2437 ,  C12N9/2445 ,  C12P19/02 ,  C12P19/14

摘要： The invention addresses the need for enzymes that can enhance the yield of fermentable sugar from the hydrolysis of lignocellulose biomass, for example sugar cane bagasse, barley straw and wheat straw, such that the use of this biomass can become economically viable. The invention provides methods for the hydrolysis of biomass using a laccase derived from Ganoderma lucidum. Further, the invention provides an enzyme composition comprising a laccase derived from Ganoderma lucidum which may be combined with one or more cellulases, and for its use in enhancing lignocellulose biomass hydrolysis.

摘要翻译： 本发明解决了对木质纤维素生物质（例如甘蔗渣，大麦秸秆和小麦秸秆）的水解可以提高可发酵糖的产量的酶的需要，使得这种生物质的使用在经济上是可行的。 本发明提供使用来自灵芝的漆酶水解生物质的方法。 此外，本发明提供了一种酶组合物，其包含衍生自灵芝的漆酶，其可与一种或多种纤维素酶组合，并用于增强木质纤维素生物质水解。