Process for the selection of PHB-producing methanotrophic cultures
    2.
    发明授权
    Process for the selection of PHB-producing methanotrophic cultures 有权
    用于选择产生PHB的甲烷营养培养物的方法

    公开(公告)号:US09062340B2

    公开(公告)日:2015-06-23

    申请号:US13590603

    申请日:2012-08-21

    IPC分类号: C12Q1/04 C12P1/00 C12P1/04

    CPC分类号: C12Q1/04

    摘要: A method of selection for type II methanotrophs is provided that includes enriching a microbial feedstock using a non-sterile bioreactor with a methane source and a nitrogen source, where the microbial feedstock includes a mixture of Type I and Type II methanotrophic cells, where an inhibited growth of the Type I methanotrophic cells and an enhanced growth of the Type II methanotrophic cells forms. The method further includes exposing intermittently the enriched microbial feedstock to i) nitrate, ii) urea, or i) and ii), where enhanced growth of the Type II methanotrophs is established, and exposing the Type II methanotrophs to an unbalanced growth condition where production of polyhydroxybutyrate is induced.

    摘要翻译: 提供了选择II型甲烷培养物的方法,其包括使用具有甲烷源和氮源的非无菌生物反应器富集微生物原料,其中微生物原料包括I型和II型甲烷营养细胞的混合物,其中被抑制 I型甲烷营养细胞的生长和II型甲烷营养细胞增殖的形成。 该方法还包括间歇地将富集的微生物原料暴露于i)硝酸盐,ii)尿素,或i)和ii),其中建立了II型甲烷营养物的增强生长,并将II型甲烷营养物暴露于不平衡的生长条件,其中生产 的多羟基丁酸酯被诱导。

    Process for the selection of PHB-producing methanotrophic cultures

    公开(公告)号:US20130052681A1

    公开(公告)日:2013-02-28

    申请号:US13590603

    申请日:2012-08-21

    IPC分类号: C12Q1/04

    CPC分类号: C12Q1/04

    摘要: A method of selection for type II methanotrophs is provided that includes enriching a microbial feedstock using a non-sterile bioreactor with a methane source and a nitrogen source, where the microbial feedstock includes a mixture of Type I and Type II methanotrophic cells, where an inhibited growth of the Type I methanotrophic cells and an enhanced growth of the Type II methanotrophic cells forms. The method further includes exposing intermittently the enriched microbial feedstock to i) nitrate, ii) urea, or i) and ii), where enhanced growth of the Type II methanotrophs is established, and exposing the Type II methanotrophs to an unbalanced growth condition where production of polyhydroxybutyrate is induced.