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1.发明申请公开(公告)号：US20170107519A1
公开(公告)日：2017-04-20
申请号：US15395348
申请日：2016-12-30
IPC分类号： C12N15/113 , C12N15/11 , C12N15/86
CPC分类号： C12N15/1132 , A61K48/00 , C12N15/111 , C12N15/113 , C12N15/1138 , C12N15/63 , C12N15/86 , C12N2310/111 , C12N2310/14 , C12N2330/30 , C12N2740/16043 , C12N2740/16045 , C12N2760/20122 , C12N2799/027 , C12N2810/609 , C12N2830/003 , C12N2830/008 , C12N2830/48 , C12N2840/203
摘要： In one aspect, the invention provides methods and compositions for the expression of small RNA molecules within a cell using a retroviral vector (FIG. 1A). Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell. In a further aspect, the invention provides methods for producing siRNA encoding lentivirus where the siRNA activity may interfere with the lentiviral life cycle. In yet a further aspect, the invention provides methods for expression of a small RNA molecule within a cell, such as an siRNA capable of downregulating CCR5, wherein expression of the small RNA molecule is relatively non-cytotoxic to the cell. The invention also includes small RNA molecules, such as an siRNA capable of downregulating CCR5, that are relatively non-cytotoxic to cells.
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2.发明授权Method for expression of small antiviral RNA molecules with reduced cytotoxicity within a cell 有权用于表达细胞内细胞毒性降低的小型抗病毒RNA分子的方法公开(公告)号：US09567592B2
公开(公告)日：2017-02-14
申请号：US14695462
申请日：2015-04-24
IPC分类号： C12N15/113 , C12N15/867 , A61P31/18 , C12N15/63 , C12N15/11 , C12N15/86 , A61K48/00
CPC分类号： C12N15/1132 , A61K48/00 , C12N15/111 , C12N15/113 , C12N15/1138 , C12N15/63 , C12N15/86 , C12N2310/111 , C12N2310/14 , C12N2330/30 , C12N2740/16043 , C12N2740/16045 , C12N2760/20122 , C12N2799/027 , C12N2810/609 , C12N2830/003 , C12N2830/008 , C12N2830/48 , C12N2840/203
摘要： In one aspect, the invention provides methods and compositions for the expression of small RNA molecules within a cell using a retroviral vector (FIG. 1A). Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell. In a further aspect, the invention provides methods for producing siRNA encoding lentivirus where the siRNA activity may interfere with the lentiviral life cycle. In yet a further aspect, the invention provides methods for expression of a small RNA molecule within a cell, such as an siRNA capable of downregulating CCR5, wherein expression of the small RNA molecule is relatively non-cytotoxic to the cell. The invention also includes small RNA molecules, such as an siRNA capable of downregulating CCR5, that are relatively non-cytotoxic to cells.
摘要翻译： 一方面，本发明提供了使用逆转录病毒载体在细胞内表达小RNA分子的方法和组合物（图1A）。 可以使用本发明的方法在细胞内表达小干扰RNA（siRNA）。 另一方面，本发明提供了用于产生编码慢病毒的siRNA的方法，其中siRNA活性可能干扰慢病毒生命周期。 在另一方面，本发明提供了在细胞内表达小RNA分子的方法，例如能够下调CCR5的siRNA，其中小RNA分子的表达对细胞是相对非细胞毒性的。 本发明还包括对细胞相对非细胞毒性的小RNA分子，例如能够下调CCR5的siRNA。
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公开(公告)号：US09551011B2
公开(公告)日：2017-01-24
申请号：US14622064
申请日：2015-02-13
IPC分类号： C12N15/867 , A61K48/00 , C12N15/86 , C12N15/113 , C12Q1/68 , A61K38/00
CPC分类号： C12N15/86 , A61K38/00 , A61K48/00 , C12N7/00 , C12N15/113 , C12N15/1132 , C12N15/1138 , C12N15/867 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2310/531 , C12N2330/30 , C12N2740/15043 , C12N2740/16043 , C12N2830/003 , C12N2830/006 , C12N2830/008 , C12N2830/30 , C12N2830/48 , C12N2830/60 , C12N2830/85 , C12N2840/20 , C12N2840/203 , C12Q1/6897 , Y02A50/465
摘要： The invention provides methods and compositions for the expression of small RNA molecules within a cell using a lentiviral vector. The methods can be used to express doubles stranded RNA complexes. Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell, which are capable of down regulating the expression of a target gene through RNA interference. A variety of cells can be treated according to the methods of the invention including embryos, embryogenic stem cells, allowing for the generation of transgenic animals or animals constituted partly by the transduced cells that have a specific gene or a group of genes down regulated.
摘要翻译： 本发明提供了使用慢病毒载体在细胞内表达小RNA分子的方法和组合物。 该方法可用于表达双链RNA复合物。 可以使用本发明的方法在细胞内表达小干扰RNA（siRNA），其能够通过RNA干扰下调靶基因的表达。 可以根据本发明的方法处理各种细胞，包括胚胎，胚胎干细胞，允许产生部分由转导细胞组成的转基因动物或动物，所述转导细胞具有特定基因或一组基因下调。
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4.发明申请公开(公告)号：US20190002883A1
公开(公告)日：2019-01-03
申请号：US16028262
申请日：2018-07-05
IPC分类号： C12N15/113 , C12N15/11 , C12N15/63 , C12N15/86 , A61K48/00
摘要： In one aspect, the invention provides methods and compositions for the expression of small RNA molecules within a cell using a retroviral vector (FIG. 1A). Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell. In a further aspect, the invention provides methods for producing siRNA encoding lentivirus where the siRNA activity may interfere with the lentiviral life cycle. In yet a further aspect, the invention provides methods for expression of a small RNA molecule within a cell, such as an siRNA capable of downregulating CCR5, wherein expression of the small RNA molecule is relatively non-cytotoxic to the cell. The invention also includes small RNA molecules, such as an siRNA capable of downregulating CCR5, that are relatively non-cytotoxic to cells.
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公开(公告)号：US09963717B2
公开(公告)日：2018-05-08
申请号：US15391189
申请日：2016-12-27
IPC分类号： C12N15/867 , C12N15/63 , A61K48/00 , C12N15/86 , C12N7/00 , C12N15/113
CPC分类号： C12N15/86 , A61K38/00 , A61K48/00 , C12N7/00 , C12N15/113 , C12N15/1132 , C12N15/1138 , C12N15/867 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2310/531 , C12N2330/30 , C12N2740/15043 , C12N2740/16043 , C12N2830/003 , C12N2830/006 , C12N2830/008 , C12N2830/30 , C12N2830/48 , C12N2830/60 , C12N2830/85 , C12N2840/20 , C12N2840/203 , C12Q1/6897 , Y02A50/465
摘要： The invention provides methods and compositions for the expression of small RNA molecules within a cell using a lentiviral vector. The methods can be used to express doubles stranded RNA complexes. Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell, which is capable of down regulating the expression of a target gene through RNA interference. A variety of cells can be treated according to the methods of the invention including embryos, embryogenic stem cells, allowing for the generation of transgenic animals or animals constituted partly by the transduced cells that have a specific gene or a group of genes down regulated.
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6.发明申请METHOD FOR EXPRESSION OF SMALL ANTIVIRAL RNA MOLECULES WITH REDUCED CYTOTOXICITY WITHIN A CELL 有权用于表达细胞中具有降低细胞毒性的小抗病毒RNA分子的方法公开(公告)号：US20150218564A1
公开(公告)日：2015-08-06
申请号：US14695462
申请日：2015-04-24
IPC分类号： C12N15/63
CPC分类号： C12N15/1132 , A61K48/00 , C12N15/111 , C12N15/113 , C12N15/1138 , C12N15/63 , C12N15/86 , C12N2310/111 , C12N2310/14 , C12N2330/30 , C12N2740/16043 , C12N2740/16045 , C12N2760/20122 , C12N2799/027 , C12N2810/609 , C12N2830/003 , C12N2830/008 , C12N2830/48 , C12N2840/203
摘要： In one aspect, the invention provides methods and compositions for the expression of small RNA molecules within a cell using a retroviral vector (FIG. 1A). Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell. In a further aspect, the invention provides methods for producing siRNA encoding lentivirus where the siRNA activity may interfere with the lentiviral life cycle. In yet a further aspect, the invention provides methods for expression of a small RNA molecule within a cell, such as an siRNA capable of downregulating CCR5, wherein expression of the small RNA molecule is relatively non-cytotoxic to the cell. The invention also includes small RNA molecules, such as an siRNA capable of downregulating CCR5, that are relatively non-cytotoxic to cells.
摘要翻译： 一方面，本发明提供了使用逆转录病毒载体在细胞内表达小RNA分子的方法和组合物（图1A）。 可以使用本发明的方法在细胞内表达小干扰RNA（siRNA）。 另一方面，本发明提供了用于产生编码慢病毒的siRNA的方法，其中siRNA活性可能干扰慢病毒生命周期。 在另一方面，本发明提供了在细胞内表达小RNA分子的方法，例如能够下调CCR5的siRNA，其中小RNA分子的表达对细胞是相对非细胞毒性的。 本发明还包括对细胞相对非细胞毒性的小RNA分子，例如能够下调CCR5的siRNA。
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公开(公告)号：US20130260453A1
公开(公告)日：2013-10-03
申请号：US13750903
申请日：2013-01-25
IPC分类号： C12N15/867 , C12N15/113 , C12Q1/68
CPC分类号： C12N15/86 , A61K38/00 , A61K48/00 , C12N7/00 , C12N15/113 , C12N15/1132 , C12N15/1138 , C12N15/867 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2310/531 , C12N2330/30 , C12N2740/15043 , C12N2740/16043 , C12N2830/003 , C12N2830/006 , C12N2830/008 , C12N2830/30 , C12N2830/48 , C12N2830/60 , C12N2830/85 , C12N2840/20 , C12N2840/203 , C12Q1/6897 , Y02A50/465
摘要： The invention provides methods and compositions for the expression of small RNA molecules within a cell using a lentiviral vector. The methods can be used to express doubles stranded RNA complexes. Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell, which are capable of down regulating the expression of a target gene through RNA interference. A variety of cells can be treated according to the methods of the invention including embryos, embryogenic stem cells, allowing for the generation of transgenic animals or animals constituted partly by the transduced cells that have a specific gene or a group of genes down regulated.
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公开(公告)号：US20170114365A1
公开(公告)日：2017-04-27
申请号：US15391189
申请日：2016-12-27
IPC分类号： C12N15/86 , C12N15/113 , C12N7/00
CPC分类号： C12N15/86 , A61K38/00 , A61K48/00 , C12N7/00 , C12N15/113 , C12N15/1132 , C12N15/1138 , C12N15/867 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2310/531 , C12N2330/30 , C12N2740/15043 , C12N2740/16043 , C12N2830/003 , C12N2830/006 , C12N2830/008 , C12N2830/30 , C12N2830/48 , C12N2830/60 , C12N2830/85 , C12N2840/20 , C12N2840/203 , C12Q1/6897 , Y02A50/465
摘要： The invention provides methods and compositions for the expression of small RNA molecules within a cell using a lentiviral vector. The methods can be used to express doubles stranded RNA complexes. Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell, which is capable of down regulating the expression of a target gene through RNA interference. A variety of cells can be treated according to the methods of the invention including embryos, embryogenic stem cells, allowing for the generation of transgenic animals or animals constituted partly by the transduced cells that have a specific gene or a group of genes down regulated.
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公开(公告)号：US20150152435A1
公开(公告)日：2015-06-04
申请号：US14622064
申请日：2015-02-13
IPC分类号： C12N15/86 , C12N15/113
CPC分类号： C12N15/86 , A61K38/00 , A61K48/00 , C12N7/00 , C12N15/113 , C12N15/1132 , C12N15/1138 , C12N15/867 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2310/531 , C12N2330/30 , C12N2740/15043 , C12N2740/16043 , C12N2830/003 , C12N2830/006 , C12N2830/008 , C12N2830/30 , C12N2830/48 , C12N2830/60 , C12N2830/85 , C12N2840/20 , C12N2840/203 , C12Q1/6897 , Y02A50/465
摘要： The invention provides methods and compositions for the expression of small RNA molecules within a cell using a lentiviral vector. The methods can be used to express doubles stranded RNA complexes. Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell, which are capable of down regulating the expression of a target gene through RNA interference. A variety of cells can be treated according to the methods of the invention including embryos, embryogenic stem cells, allowing for the generation of transgenic animals or animals constituted partly by the transduced cells that have a specific gene or a group of genes down regulated.
摘要翻译： 本发明提供了使用慢病毒载体在细胞内表达小RNA分子的方法和组合物。 该方法可用于表达双链RNA复合物。 可以使用本发明的方法在细胞内表达小干扰RNA（siRNA），其能够通过RNA干扰下调靶基因的表达。 可以根据本发明的方法处理各种细胞，包括胚胎，胚胎干细胞，允许产生部分由转导细胞组成的转基因动物或动物，所述转导细胞具有特定基因或一组基因下调。
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10.发明授权Method for expression of small antiviral RNA molecules with reduced cytotoxicity within a cell 有权用于表达细胞内细胞毒性降低的小型抗病毒RNA分子的方法公开(公告)号：US09034577B2
公开(公告)日：2015-05-19
申请号：US13749319
申请日：2013-01-24
IPC分类号： C12N15/86 , C12N15/867 , C07H21/02 , C12N15/11 , C12N15/113 , A61K48/00
CPC分类号： C12N15/1132 , A61K48/00 , C12N15/111 , C12N15/113 , C12N15/1138 , C12N15/63 , C12N15/86 , C12N2310/111 , C12N2310/14 , C12N2330/30 , C12N2740/16043 , C12N2740/16045 , C12N2760/20122 , C12N2799/027 , C12N2810/609 , C12N2830/003 , C12N2830/008 , C12N2830/48 , C12N2840/203
摘要： In one aspect, the invention provides methods and compositions for the expression of small RNA molecules within a cell using a retroviral vector (FIG. 1A). Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell. In a further aspect, the invention provides methods for producing siRNA encoding lentivirus where the siRNA activity may interfere with the lentiviral life cycle. In yet a further aspect, the invention provides methods for expression of a small RNA molecule within a cell, such as an siRNA capable of downregulating CCR5, wherein expression of the small RNA molecule is relatively non-cytotoxic to the cell. The invention also includes small RNA molecules, such as an siRNA capable of downregulating CCR5, that are relatively non-cytotoxic to cells.
摘要翻译： 一方面，本发明提供了使用逆转录病毒载体在细胞内表达小RNA分子的方法和组合物（图1A）。 可以使用本发明的方法在细胞内表达小干扰RNA（siRNA）。 另一方面，本发明提供了用于产生编码慢病毒的siRNA的方法，其中siRNA活性可能干扰慢病毒生命周期。 在另一方面，本发明提供了在细胞内表达小RNA分子的方法，例如能够下调CCR5的siRNA，其中小RNA分子的表达对细胞是相对非细胞毒性的。 本发明还包括对细胞相对非细胞毒性的小RNA分子，例如能够下调CCR5的siRNA。
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