公开(公告)号：US20240288409A1

公开(公告)日：2024-08-29

申请号：US18571782

申请日：2022-06-20

申请人： CRESCENDO CARE S.R.L.

发明人： Francesco Chiara ,  Simona Roggero

IPC分类号： G01N30/88 ,  G01N30/02 ,  G01N30/06 ,  G01N30/72

CPC分类号： G01N30/88 ,  G01N30/06 ,  G01N30/72 ,  G01N2030/027 ,  G01N2030/062 ,  G01N2030/8818 ,  G01N2030/8822 ,  G01N2030/8827

摘要： The present invention relates to a multiresidual method for detecting and/or quantifying at least one amino acid or derivative thereof, at least one organic acid, and/or at least one modified nucleotide, in a sample of biological liquid or circulating cells, comprising the steps of: a) treating the sample with an extraction mixture at room temperature, preferably refrigerated at a temperature lower than −20° C., comprising i) a mixture of one or more organic solvents having a final polarity index between 3 and 6, ii) a strong acid in an amount sufficient for the extraction mixture to have a normality from 0.005 to 0.025 N or a weak acid with ka in the range between 3.5×10−7 and 7.0×10−3 with a final concentration in the extraction mixture from 5 to 30 mM; wherein the extraction mixture comprises: acetonitrile, dichioromethane and formic acid; or acetonitrile and hydrochloric acid; or acetone and formic acid; or methanol and formic acid; or acetonitrile and formic acid; or methanol and dimethyl sulfoxide; or acetonitrile and dimethyl sulfoxide; or acetonitrile, methanol and hydrochloric acid; or acetonitrile and methanol; b) performing a hydrophilic interaction liquid chromatography (HILIC) on the sample treated in step a); c) performing an analysis by tandem mass spectrometry on the sample obtained in step b) detecting and/or quantifying the at least one amino acid or derivative thereof, and/or the at least one organic acid, and/or the at least one modified nucleotide.