-
公开(公告)号：US20190338323A1
公开(公告)日：2019-11-07
申请号：US16514518
申请日：2019-07-17
发明人： Howard CHOU , Xiucai Liu
IPC分类号： C12P13/08 , C12P13/00 , C12N9/88 , C07K14/36 , C07K14/245 , C12N15/77 , C12N15/70 , C07K14/35
摘要： One aspect of the invention relates to a mutant polypeptide comprising the amino acid sequence of Escherichia coli tetracycline efflux pump A (TetA). Another aspect of the invention relates to a polynucleotide encoding a polypeptide of TetA, a fragment thereof, or a mutant thereof. Another aspect of the invention relates to a first expression plasmid vector comprising one or more first polynucleotides encoding a first polypeptide comprising a tetracycline efflux pump polypeptide, a fragment thereof or a mutant thereof, one or more second polynucleotides independently selected from the group consisting of a third polynucleotide encoding a third polypeptide comprising a lysine decarboxylase polypeptide, a fragment thereof or a mutant thereof, and a fourth polynucleotide encoding a fourth polypeptide comprising a lysine biosynthesis polypeptide, a fragment thereof or a mutant thereof. Another aspect of the invention relates to a transformant comprising one or more expression plasmid vectors as described herein in a host cell. Another aspect of the invention relates to a mutant host cell comprising one or more first, third or fourth polynucleotides as described herein integrated into a chromosome of a host cell. Another aspect of the invention relates to a method for producing a lysine comprising obtaining a transformant and/or mutant host cell as disclosed herein, culturing the transformant and/or mutant host cell under conditions effective for the expression of the lysine; and harvesting the lysine. Other aspects of the invention relate to methods for producing biobased cadaverine using the transformants disclosed herein, and biobased cadaverine prepared by the method disclosed herein, and polyamides formed using the biobased cadaverine as disclosed herein and compositions thereof.
-
公开(公告)号：US20190330613A1
公开(公告)日：2019-10-31
申请号：US16464104
申请日：2016-11-24
发明人： Howard Chou , Ling Chen , Wenqiang Lu , Xiucai Liu
摘要： This invention provides acid decarboxylase-prion subunit fusion polypeptides, nucleic acid sequences, expression vectors, and host cells expression such fusion polypeptides to produce various amino acids and derivatives of the amino acids such as polyamines.
-
公开(公告)号：US10464880B2
公开(公告)日：2019-11-05
申请号：US15901475
申请日：2018-02-21
发明人： Xiucai Liu , Charlie Liu , Bingbing Qin , Naiqiang Li , Yang Chen
IPC分类号： C07C209/84 , C07C211/09 , B01D21/26 , B01D3/34 , B01D1/00 , B01D3/40
摘要： The present invention relates to a method for the purification of cadaverine from an aqueous cadaverine salt composition comprising one or more involatile impurities, the method comprising: (1) mixing the aqueous cadaverine salt composition with one or more bases to provide an aqueous cadaverine composition having a pH of at least about 12; (2) distilling or evaporating the aqueous cadaverine composition to produce an aqueous cadaverine solution, wherein one or more solvents are added to the evaporation/distillation system before the evaporation/distillation stops, and the one or more solvents comprise at least one or more high boiling point (HBP) solvents having a boiling point of at least 185° C. (1 atm); and (3) subjecting the aqueous cadaverine solution to one or more rectification steps to provide a cadaverine product.
-
4.发明授权公开(公告)号：US10221407B2
公开(公告)日：2019-03-05
申请号：US14992412
申请日：2016-01-11
发明人： Zhenhua Pang , Naiqiang Li , Charlie Liu , Xiucai Liu
IPC分类号： C12N9/88 , C12N15/74 , C07C211/09 , C12P13/00 , C08G69/26
摘要： One aspect of the present disclosure relates to a stabilized recombinant expression plasmid vector comprising a polynucleotide encoding an antitoxin gene which expresses a polypeptide that neutralizes a polypeptide toxic to a host cell, the toxic polypeptide being expressed by a toxin gene in the host cell, and a polynucleotide encoding a polypeptide expression product, and the stabilized recombinant expression plasmid vector is derived from a Hafnia alvei autonomously replicable backbone plasmid. Other aspects of the present disclosure relate to a transformant transformed with the stabilized recombinant expression plasmid vector disclosed herein, a method of producing biobased cadaverine using the transformant disclosed herein, and biobased cadaverine prepared by the method disclosed herein. Another aspect of the present disclosure relates to a polyamide formed using biobased cadaverine disclosed herein, and a composition thereof. Another aspect of the present disclosure relates to a method of preparing 1,5-diisocyanatopentane comprising preparing biobased cadaverine using the method disclosed herein and converting the biobased cadaverine to 1,5-diisocyanatopentane.
-
公开(公告)号：US20190031726A1
公开(公告)日：2019-01-31
申请号：US15774820
申请日：2015-11-09
发明人： Howard Chou , Lijun Yu , Xiucai Liu
IPC分类号： C07K14/245 , C12N9/88 , C12P13/08 , C12N15/70
摘要： Provided are microorganisms genetically modified to overexpress porin polypeptides to enhance the production of lysine and lysine derivatives by the microorganism. Also provided are methods of generating such microorganism, and methods of producing lysine and lysine derivatives using the genetically modified microorganisms.
-
公开(公告)号：US09556106B2
公开(公告)日：2017-01-31
申请号：US14764109
申请日：2013-01-28
发明人： Xiucai Liu , Charlie Liu , Duanfang Dai , Bingbing Qin , Naiqiang Li
IPC分类号： C07C209/84 , C12P13/00
CPC分类号： C07C209/84 , C12P13/001 , C07C211/09
摘要： One aspect of the invention relates to a method for the purification of cadaverine from an aqueous cadaverine composition comprising one or more involatile impurities, the method comprising obtaining cadaverine from evaporation or distillation of the aqueous cadaverine composition wherein one or more solvents are added to the evaporation/distillation system before the evaporation/distillation starts, during the evaporation/distillation and/or after the evaporation/distillation substantially stops when no more evaporation/distillation is observed; wherein the one or more solvents comprise at least one or more high boiling point (HBP) solvents. In certain embodiments, cadaverine may be evaporated/distilled at an unexpectedly lower heating temperature for an unexpectedly shorter heating time to provide a desired yield. Because cadaverine may decompose at high temperature, the unexpectedly lower heating temperature and unexpectedly shorter heating time decrease the undesired cadaverine decomposition. The methods disclosed herein provides unexpected high recovery rate (˜100%) in the evaporation/distillation of cadaverine at a larger scale at a lower heating temperature compared to conventional distillation methods. Thus, the method disclosed herein will be highly appreciated in the industrial production of cadaverine.
摘要翻译： 本发明的一个方面涉及从包含一种或多种非挥发性杂质的水性尸胺组合物中纯化尸胺的方法，所述方法包括从蒸发或蒸馏水性尸胺组合物中获得尸胺，其中将一种或多种溶剂加入到蒸发中 在蒸发/蒸馏开始之前，蒸发/蒸馏期间和/或蒸发/蒸馏之后蒸馏/蒸馏系统在不再观察到蒸发/蒸馏的情况下基本停止; 其中所述一种或多种溶剂包含至少一种或多种高沸点（HBP）溶剂。 在某些实施方案中，可以在意想不到的较低加热温度下将尸胺蒸发/蒸馏，以期意外地更短的加热时间以提供所需的产率。 因为尸体可能会在高温下分解，出人意料地降低加热温度，而且意外的加热时间会缩短，从而减少不希望的尸体分解。 与常规蒸馏方法相比，本文所公开的方法在较低的加热温度下在较大规模的蒸发/蒸馏中提供了意想不到的高回收率（〜100％）。 因此，本文公开的方法将在尸胺的工业生产中被高度赞赏。
-
7.发明授权Stabilized recombinant expression plasmid vector in hafnia alvei and applications thereof 有权稳定的重组表达质粒载体在hafnia alvei及其应用公开(公告)号：US09234203B2
公开(公告)日：2016-01-12
申请号：US13795949
申请日：2013-03-12
发明人： Zhenhua Pang , Naiqiang Li , Charlie Liu , Xiucai Liu
CPC分类号： C12N9/88 , C07C211/09 , C08G69/26 , C12N15/74 , C12P13/00 , C12P13/001 , C12Y401/01018
摘要： One aspect of the present disclosure relates to a stabilized recombinant expression plasmid vector comprising a polynucleotide encoding an antitoxin gene which expresses a polypeptide that neutralizes a polypeptide toxic to a host cell, the toxic polypeptide being expressed by a toxin gene in the host cell, and a polynucleotide encoding a polypeptide expression product, and the stabilized recombinant expression plasmid vector is derived from a Hafnia alvei autonomously replicable backbone plasmid. Other aspects of the present disclosure relate to a transformant transformed with the stabilized recombinant expression plasmid vector disclosed herein, a method of producing biobased cadaverine using the transformant disclosed herein, and biobased cadaverine prepared by the method disclosed herein. Another aspect of the present disclosure relates to a polyamide formed using biobased cadaverine disclosed herein, and a composition thereof. Another aspect of the present disclosure relates to a method of preparing 1,5-diisocyanatopentane comprising preparing biobased cadaverine using the method disclosed herein and converting the biobased cadaverine to 1,5-diisocyanatopentane.
摘要翻译： 本公开的一个方面涉及包含编码抗毒素基因的多核苷酸的稳定的重组表达质粒载体，所述多核苷酸表达中和宿主细胞毒性多肽的多肽，所述毒性多肽由所述宿主细胞中的毒素基因表达，以及 编码多肽表达产物的多核苷酸和稳定的重组表达质粒载体衍生自Hafnia alvei自主复制主链质粒。 本公开的其它方面涉及用本文公开的稳定的重组表达质粒载体转化的转化体，使用本文公开的转化体生产生物基的尸胺的方法，以及通过本文公开的方法制备的生物基尸胺。 本公开的另一方面涉及使用本文公开的生物基尸胺形成的聚酰胺及其组合物。 本公开的另一方面涉及制备1,5-二异氰酸酯戊烷的方法，其包括使用本文公开的方法制备生物基尸胺，并将生物基尸胺转化为1,5-二异氰酸根戊烷。
-
公开(公告)号：US10584149B2
公开(公告)日：2020-03-10
申请号：US15547323
申请日：2015-01-30
发明人： Howard Chou , Naiqiang Li , Xiucai Liu
摘要： Provided are lysine decarboxylase polypeptides comprising mutants of SEQ ID NO: 2 and/or fragments thereof. The mutants or fragments have at least 95% sequence identity with SEQ ID NO: 2. Also provided are DNA polynucleotides encoding said lysine decarboxylases, expression vector comprising the DNA polynucleotides, transformants, mutant host cells, methods for the production of lysine decarboxylases, and methods for the production of a lysine-derived product.
-
公开(公告)号：US10400257B2
公开(公告)日：2019-09-03
申请号：US15517633
申请日：2014-10-09
发明人： Howard Chou , Xiucai Liu
IPC分类号： C12P13/08 , C12P13/00 , C12N9/88 , C07K14/36 , C07K14/35 , C07K14/245 , C12N15/77 , C12N15/70
摘要： One aspect of the invention relates to a mutant polypeptide comprising the amino acid sequence of Escherichia coli tetracycline efflux pump A (TetA). Another aspect of the invention relates to a polynucleotide encoding a polypeptide of TetA, a fragment thereof, or a mutant thereof. Another aspect of the invention relates to a first expression plasmid vector comprising one or more first polynucleotides encoding a first polypeptide comprising a tetracycline efflux pump polypeptide, a fragment thereof or a mutant thereof, one or more second polynucleotides independently selected from the group consisting of a third polynucleotide encoding a third polypeptide comprising a lysine decarboxylase polypeptide, a fragment thereof or a mutant thereof, and a fourth polynucleotide encoding a fourth polypeptide comprising a lysine biosynthesis polypeptide, a fragment thereof or a mutant thereof. Another aspect of the invention relates to a transformant comprising one or more expression plasmid vectors as described herein in a host cell. Another aspect of the invention relates to a mutant host cell comprising one or more first, third or fourth polynucleotides as described herein integrated into a chromosome of a host cell. Another aspect of the invention relates to a method for producing a lysine comprising obtaining a transformant and/or mutant host cell as disclosed herein, culturing the transformant and/or mutant host cell under conditions effective for the expression of the lysine; and harvesting the lysine. Other aspects of the invention relate to methods for producing biobased cadaverine using the transformants disclosed herein, and biobased cadaverine prepared by the method disclosed herein, and polyamides formed using the biobased cadaverine as disclosed herein and compositions thereof.
-
公开(公告)号：US09914694B2
公开(公告)日：2018-03-13
申请号：US15418052
申请日：2017-01-27
发明人： Xiucai Liu , Charlie Liu , Bingbing Qin , Naiqiang Li , Yang Chen
IPC分类号： C07C209/84
CPC分类号： C07C209/84 , C07C211/09
摘要： The present invention relates to a method for the purification of cadaverine from an aqueous cadaverine salt composition comprising one or more involatile impurities, the method comprising: (1) mixing the aqueous cadaverine salt composition with one or more bases selected from the group consisting of Ca(OH)2, Mg(OH)2, CaO and MgO to provide an aqueous cadaverine composition; and (2) distilling or evaporating the aqueous cadaverine composition to produce purified cadaverine; wherein one or more solvents are added to the evaporation/distillation system before the evaporation/distillation stops, and the one or more solvents comprise at least one or more high boiling point (HBP) solvents.
-
-
-
-
-
-
-
-
-
搜索结果
64 条记录 (耗时 0.026 秒)
