发明授权
US09234203B2 Stabilized recombinant expression plasmid vector in hafnia alvei and applications thereof
有权
稳定的重组表达质粒载体在hafnia alvei及其应用
- 专利标题: Stabilized recombinant expression plasmid vector in hafnia alvei and applications thereof
- 专利标题(中): 稳定的重组表达质粒载体在hafnia alvei及其应用
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申请号: US13795949申请日: 2013-03-12
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公开(公告)号: US09234203B2公开(公告)日: 2016-01-12
- 发明人: Zhenhua Pang , Naiqiang Li , Charlie Liu , Xiucai Liu
- 申请人: CATHAY INDUSTRIAL BIOTECH LTD. , CATHAY R&D CENTER CO., LTD.
- 申请人地址: KY Georgetown CN Shanghai
- 专利权人: Cathay Industrial Biotech Ltd.,Cathay R&D Center Co., Ltd.
- 当前专利权人: Cathay Industrial Biotech Ltd.,Cathay R&D Center Co., Ltd.
- 当前专利权人地址: KY Georgetown CN Shanghai
- 代理机构: Perkins Coie LLP
- 代理商 Yingli Wang
- 主分类号: C12N15/74
- IPC分类号: C12N15/74 ; C12P13/00 ; C08G69/26
摘要:
One aspect of the present disclosure relates to a stabilized recombinant expression plasmid vector comprising a polynucleotide encoding an antitoxin gene which expresses a polypeptide that neutralizes a polypeptide toxic to a host cell, the toxic polypeptide being expressed by a toxin gene in the host cell, and a polynucleotide encoding a polypeptide expression product, and the stabilized recombinant expression plasmid vector is derived from a Hafnia alvei autonomously replicable backbone plasmid. Other aspects of the present disclosure relate to a transformant transformed with the stabilized recombinant expression plasmid vector disclosed herein, a method of producing biobased cadaverine using the transformant disclosed herein, and biobased cadaverine prepared by the method disclosed herein. Another aspect of the present disclosure relates to a polyamide formed using biobased cadaverine disclosed herein, and a composition thereof. Another aspect of the present disclosure relates to a method of preparing 1,5-diisocyanatopentane comprising preparing biobased cadaverine using the method disclosed herein and converting the biobased cadaverine to 1,5-diisocyanatopentane.
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