公开(公告)号：US20230354790A1

公开(公告)日：2023-11-09

申请号：US18246250

申请日：2021-09-28

申请人： Brandeis University

发明人： Paul Allen Garrity ,  Willem Jeffrey Laursen

IPC分类号： A01K67/033 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90 ,  C07K14/435

CPC分类号： A01K67/0339 ,  C12N9/22 ,  C12N15/111 ,  C12N15/902 ,  C07K14/43563 ,  C12N2310/20 ,  C12N2800/80 ,  A01K2227/706 ,  A01K2217/072 ,  A01K2267/02

摘要： A method of making sterile diploid organisms includes mating a first population and a second population of single knock-in diploid organisms, wherein the first population of single knock-in diploid organisms are heterozygous organisms expressing a first marker inserted into a gene required for fertility, wherein the second population of single knock-in diploid organisms are heterozygous organisms expressing a second marker inserted into the gene required for fertility, wherein introduction of the first/second marker disrupts expression of the required fertility gene creating a first/second mutant allele of the gene required for fertility, and wherein the first and second markers are distinct; sorting offspring produced from the mating based on their expression of the first and/or second markers; and isolating the sterile diploid organisms, wherein the sterile diploid organisms are heteroallelic diploid organisms expressing the first marker in the first mutant allele and the second marker in the mutant second allele.