公开(公告)号：US20200277341A1

公开(公告)日：2020-09-03

申请号：US16606958

申请日：2018-04-19

Applicant: Biological E Limited

Inventor： Balamurali Masilamani ,  Rajan Sriraman ,  Mandar Shirish Dixit ,  Deviprasanna Chakka ,  Satyam Naidu Sureddi ,  Ramesh Venkat Matur ,  Narender Dev Mantena ,  Mahima Datla

IPC: C07K14/34 ,  C12N1/20 ,  C12P21/00 ,  A61K39/05 ,  A61K39/385

Abstract: The present invention provides an improved method for the production of CRM197 with high yield using engineered Corynebacterium diphtheria strain having an increased copy number of the CRM197 gene, wherein the method comprises growing the strain in media free of animal-derived components with one or more amino acids.

公开(公告)号：US11098089B2

公开(公告)日：2021-08-24

申请号：US16606958

申请日：2018-04-19

Applicant: Biological E Limited

Inventor： Balamurali Masilamani ,  Rajan Sriraman ,  Mandar Shirish Dixit ,  Deviprasanna Chakka ,  Satyam Naidu Sureddi ,  Ramesh Venkat Matur ,  Narender Dev Mantena ,  Mahima Datla

IPC: C12N1/20 ,  A61K39/05 ,  A61K39/385 ,  C12P21/00 ,  C07K14/34

Abstract: The present invention provides an improved method for the production of CRM197 with high yield using engineered Corynebacterium diphtheria strain having an increased copy number of the CRM197 gene, wherein the method comprises growing the strain in media free of animal-derived components with one or more amino acids.