公开(公告)号：US09896717B2

公开(公告)日：2018-02-20

申请号：US14272370

申请日：2014-05-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Shawn Hodges ,  Nick Heredia ,  Jonathan Petersen

IPC: G01N33/53 ,  C12Q1/68

CPC classification number: C12Q1/6804 ,  C12Q2537/159 ,  C12Q2561/101 ,  C12Q2563/143 ,  C12Q2563/149

Abstract: The present invention provides methods of detecting an antigen in a sample comprising contacting the sample with affinity agents to form an antigen-affinity agent-label complex, separating the antigen-affinity agent-label complex from uncomplexed components, partitioning label from the separated sample into multiple partitions, and detecting the presence of the label in one or more partitions. Compositions and kits for detecting an antigen in a sample are also provided.

公开(公告)号：US20170191127A1

公开(公告)日：2017-07-06

申请号：US15394396

申请日：2016-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Shawn Hodges ,  Nicholas Heredia

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6874 ,  C12N15/1068 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/686 ,  C40B50/06 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/159 ,  C12Q2563/159

Abstract: Methods of preparing a target gene-enriched library are provided. In one aspect, the method comprises partitioning polynucleotide fragments into a plurality of partitions, wherein each partition further comprises a plurality of primer pairs for amplifying a target gene and wherein the primers comprise a portion of an adapter sequence; amplifying a target gene sequence to generate an amplicon comprising the target gene sequence flanked on either end by a portion of an adapter sequence; purifying the amplicon; and amplifying the amplicon using primers comprising full-length adapter sequences.

公开(公告)号：US20140336068A1

公开(公告)日：2014-11-13

申请号：US14272370

申请日：2014-05-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Shawn Hodges ,  Nick Heredia ,  Jonathan Petersen

IPC: C12Q1/68

CPC classification number: C12Q1/6804 ,  C12Q2537/159 ,  C12Q2561/101 ,  C12Q2563/143 ,  C12Q2563/149

Abstract: The present invention provides methods of detecting an antigen in a sample comprising contacting the sample with affinity agents to form an antigen-affinity agent-label complex, separating the antigen-affinity agent-label complex from uncomplexed components, partitioning label from the separated sample into multiple partitions, and detecting the presence of the label in one or more partitions. Compositions and kits for detecting an antigen in a sample are also provided.

Abstract translation: 本发明提供检测样品中的抗原的方法，包括使样品与亲和试剂接触以形成抗原亲和试剂 - 标记复合物，将抗原亲和试剂 - 标记复合物与未络合的组分分离，将标记从分离的样品分离成 多个分区，并且检测标签在一个或多个分区中的存在。 还提供了用于检测样品中的抗原的组合物和试剂盒。