公开(公告)号：US10099157B2

公开(公告)日：2018-10-16

申请号：US14747221

申请日：2015-06-23

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Mark A. Snyder ,  Kimberly C. Brisack

IPC: G01N30/50 ,  B01J20/281 ,  B01D15/20 ,  B01D15/36 ,  C01B25/32 ,  C01B25/455 ,  G01N30/26 ,  B01D15/38 ,  B01D15/42 ,  B01J20/04 ,  B01J20/30 ,  B01J20/34

Abstract: Disclosed are compositions and methods for regenerating or reducing the deterioration of an apatite solid surface during, or subsequent to, a chromatographic procedure for purifying a target molecule from a sample, by treating the apatite solid surface with a buffered calcium solution, followed by a phosphate buffered solution, followed by an alkaline hydroxide. The buffered calcium solution, phosphate buffered solution, and alkaline hydroxide can be applied subsequent to a bind and elute or flow through purification procedure. The methods provide an increase in resin mass and/or particle strength compared to prior methods.

公开(公告)号：US20150367252A1

公开(公告)日：2015-12-24

申请号：US14747221

申请日：2015-06-23

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Mark A. Snyder ,  Kimberly C. Brisack

IPC: B01D15/20 ,  B01D15/36

CPC classification number: B01D15/203 ,  B01D15/361 ,  B01D15/3847 ,  B01J20/048 ,  B01J20/282 ,  B01J20/3085 ,  B01J20/34 ,  B01J20/3433 ,  B01J20/3475 ,  C01B25/32 ,  C01B25/327 ,  C01B25/455 ,  G01N30/26 ,  G01N30/48 ,  G01N30/50

Abstract: Disclosed herein are methods of regenerating apatite surfaces, for example after purification of a target analyte.

Abstract translation: 本文公开的是再生磷灰石表面的方法，例如在纯化目标分析物之后。