公开(公告)号：US09856503B2

公开(公告)日：2018-01-02

申请号：US14453830

申请日：2014-08-07

申请人： BIOMERIEUX, INC.

发明人： Ronnie J. Robinson ,  Mark S. Wilson ,  Christopher S. Ronsick ,  John D. Walsh ,  Jones M. Hyman ,  Thurman C. Thorpe ,  Bradford G. Clay

IPC分类号： G01N35/00 ,  C12M1/00 ,  B01L3/00 ,  C12Q1/04 ,  C12M1/34 ,  C12Q1/02 ,  G01N35/04

CPC分类号： C12Q1/04 ,  C12M41/36 ,  C12Q1/02 ,  G01N35/00603 ,  G01N35/0099 ,  G01N35/026 ,  G01N2035/00148 ,  G01N2035/0406 ,  G01N2035/0453 ,  G01N2035/0465

摘要： A detection instrument determines whether a specimen container (e.g., blood culture bottle) is positive for presence of microbial agent growth therein. When the container is deemed positive it is made available (e.g, transferred or exposed to) to an automated instrument performing identification and/or characterization of the microbial agent. The identification and/or characterization instrument removes a portion of the sample from the specimen container and places it into a disposable separation and concentration device. The microbial agent is concentrated via optional selective lysis of non-microbial agent cellular material which may be present and centrifugation. A reading module reads the concentrated microbial agent using spectroscopic methods, e.g., measurements of intrinsic fluorescence. Such interrogation may occur while the microbial agent remains concentrated in the disposable device.

公开(公告)号：US20160349178A1

公开(公告)日：2016-12-01

申请号：US15166768

申请日：2016-05-27

申请人： bioMerieux, Inc.

发明人： John D. Walsh ,  Christopher S. Ronsick ,  Jones M. Hyman ,  Mark S. Wilson

IPC分类号： G01N21/47 ,  C12Q1/04

CPC分类号： C12Q1/04 ,  G01N21/272 ,  G01N21/51

摘要： A device and method for determining antimicrobial resistance status of a microorganism from a positive sample bottle is provided. The device may include a housing having a chamber for receiving a vial containing a sample; a light source positioned to direct light through a side of the chamber; and a photodetector positioned such that light transmitted or scattered by the sample is sensed by the photodetector. The device may also include a heat source and/or an agitation device. The method includes providing the device, loading a sample from an automated sample culture system into the vial; interrogating the vial using the light source and the photodetector; and determining the antimicrobial resistance status of the microorganism in the sample based on the interrogating step. The method may also include heating and/or agitating the sample.

摘要翻译： 提供了一种用于从阳性样品瓶中测定微生物的抗微生物抗性状态的装置和方法。 该装置可以包括具有用于接收包含样品的小瓶的室的壳体; 定位成将光引导通过所述室的一侧的光源; 以及光电检测器，其被定位成使得由光电检测器感测由样品透射或散射的光。 该装置还可以包括热源和/或搅拌装置。 该方法包括提供装置，将样品从自动化样品培养系统装载到小瓶中; 使用光源和光电检测器询问小瓶; 以及基于询问步骤确定样品中的微生物的抗微生物抗性状态。 该方法还可以包括加热和/或搅拌样品。

公开(公告)号：US10184144B2

公开(公告)日：2019-01-22

申请号：US14156725

申请日：2014-01-16

申请人： BIOMERIEUX, INC

发明人： Michael Ullery ,  Erin Mathias ,  Jones M. Hyman ,  John D. Walsh

IPC分类号： G01N33/50 ,  C12Q1/04 ,  G01N21/64 ,  G06F19/24

摘要： A method for identification and/or characterization of a microbial agent present in a sample includes a step of analytical test data (e.g., obtaining intrinsic fluorescence values over a range of emission wavelengths) from the microbial agent. The analytical test data is transformed thereby minimizing strain to strain variations within an organism group. With the aid of a programmed computer, a multi-level classification algorithm coded as a set of processing instructions operates on the transformed analytic test data. The multiple levels correspond to different levels in a taxonomic hierarchy for microbial agents suspected of being in the sample.

公开(公告)号：US10214764B2

公开(公告)日：2019-02-26

申请号：US14882099

申请日：2015-10-13

申请人： bioMerieux, Inc.

发明人： John Walsh ,  Jones M. Hyman ,  Christopher Ronsick ,  John Link ,  Ron Robinson ,  Mark Wilson

IPC分类号： B01L1/00 ,  B01L3/00 ,  C12Q1/24 ,  C12Q1/04 ,  G01N21/65 ,  G01N33/68 ,  G01N21/03 ,  C12Q1/37 ,  C12Q1/6816 ,  G01N33/487 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/359 ,  G01N33/49

摘要： The present invention is directed to a separation device or container that can be used in the separation, isolation or pelleting of microorganisms from a test samples known to contain or suspected of containing said microorganisms. Subsequently, the separated, isolated or pelleted microorganism sample can undergo one or more interrogation steps to provide measurements useful for the characterization and/or identification of microorganism. In one aspect of the present invention, the interrogation steps can occur in situ in the separation device or container described herein.

公开(公告)号：US10053721B2

公开(公告)日：2018-08-21

申请号：US15166768

申请日：2016-05-27

申请人： bioMerieux, Inc.

发明人： John D. Walsh ,  Christopher S. Ronsick ,  Jones M. Hyman ,  Mark S. Wilson

IPC分类号： C12M1/02 ,  C12Q1/04 ,  G01N21/27 ,  G01N21/51

CPC分类号： C12Q1/04 ,  G01N21/272 ,  G01N21/51

摘要： A device and method for determining antimicrobial resistance status of a microorganism from a positive sample bottle is provided. The device may include a housing having a chamber for receiving a vial containing a sample; a light source positioned to direct light through a side of the chamber; and a photodetector positioned such that light transmitted or scattered by the sample is sensed by the photodetector. The device may also include a heat source and/or an agitation device. The method includes providing the device, loading a sample from an automated sample culture system into the vial; interrogating the vial using the light source and the photodetector; and determining the antimicrobial resistance status of the microorganism in the sample based on the interrogating step. The method may also include heating and/or agitating the sample.

公开(公告)号：US20160032348A1

公开(公告)日：2016-02-04

申请号：US14882099

申请日：2015-10-13

申请人： bioMerieux, Inc.

发明人： John Walsh ,  Jones M. Hyman ,  Christopher Ronsick ,  John Link ,  Ronnie Robinson ,  Mark Wilson

IPC分类号： C12Q1/04 ,  B01L3/00 ,  C12Q1/24

CPC分类号： C12Q1/24 ,  B01L3/5021 ,  B01L2300/042 ,  B01L2300/0838 ,  B01L2300/0861 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/6816 ,  G01N21/03 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/48735 ,  G01N33/491 ,  G01N33/6848

摘要： The present invention is directed to a separation device or container that can be used in the separation, isolation or pelleting of microorganisms from a test samples known to contain or suspected of containing said microorganisms. Subsequently, the separated, isolated or pelleted microorganism sample can undergo one or more interrogation steps to provide measurements useful for the characterization and/or identification of microorganism. In one aspect of the present invention, the interrogation steps can occur in situ in the separation device or container described herein.

摘要翻译： 本发明涉及可用于从已知含有或怀疑含有所述微生物的测试样品中分离，分离或造粒微生物的分离装置或容器。 随后，分离的，分离的或沉淀的微生物样品可以经历一个或多个询问步骤以提供对微生物的表征和/或鉴定有用的测量。 在本发明的一个方面，询问步骤可以在本文所述的分离装置或容器中原位发生。

公开(公告)号：US20150031074A1

公开(公告)日：2015-01-29

申请号：US14453830

申请日：2014-08-07

申请人： BIOMERIEUX, INC.

发明人： Ronnie J. Robinson ,  Mark S. Wilson ,  Christopher S. Ronsick ,  John D. Walsh ,  Jones M. Hyman ,  Thurman C. Thorpe ,  Bradford G. Clay

IPC分类号： C12Q1/04 ,  G01N35/00

CPC分类号： C12Q1/04 ,  C12M41/36 ,  C12Q1/02 ,  G01N35/00603 ,  G01N35/0099 ,  G01N35/026 ,  G01N2035/00148 ,  G01N2035/0406 ,  G01N2035/0453 ,  G01N2035/0465

摘要： A detection instrument determines whether a specimen container (e.g., blood culture bottle) is positive for presence of microbial agent growth therein. When the container is deemed positive it is made available (e.g, transferred or exposed to) to an automated instrument performing identification and/or characterization of the microbial agent. The identification and/or characterization instrument removes a portion of the sample from the specimen container and places it into a disposable separation and concentration device. The microbial agent is concentrated via optional selective lysis of non-microbial agent cellular material which may be present and centrifugation. A reading module reads the concentrated microbial agent using spectroscopic methods, e.g., measurements of intrinsic fluorescence. Such interrogation may occur while the microbial agent remains concentrated in the disposable device.

摘要翻译： 检测仪器确定样品容器（例如血培养瓶）是否对其中存在微生物剂生长是阳性的。 当容器被认为是正的时，它被提供（例如，转移或暴露于）到执行微生物剂的鉴定和/或表征的自动化仪器。 识别和/或表征仪器将样品的一部分从样品容器中取出并将其放入一次性分离和浓缩装置中。 微生物剂通过选择性裂解可能存在的和非离子的非微生物剂细胞材料而浓缩。 阅读模块使用光谱方法读取浓缩的微生物剂，例如固有荧光的测量。 当微生物剂保持集中在一次性装置中时，可能发生这种询问。

公开(公告)号：US20140134670A1

公开(公告)日：2014-05-15

申请号：US14156725

申请日：2014-01-16

申请人： BIOMERIEUX, INC.

发明人： Michael Ullery ,  Erin Mathias ,  Jones M. Hyman ,  John D. Walsh

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04 ,  G01N21/6486 ,  G06F19/24

摘要： A method for identification and/or characterization of a microbial agent present in a sample includes a step of analytical test data (e.g., obtaining intrinsic fluorescence values over a range of emission wavelengths) from the microbial agent. The analytical test data is transformed thereby minimizing strain to strain variations within an organism group. With the aid of a programmed computer, a multi-level classification algorithm coded as a set of processing instructions operates on the transformed analytic test data. The multiple levels correspond to different levels in a taxonomic hierarchy for microbial agents suspected of being in the sample

摘要翻译： 用于鉴定和/或表征样品中存在的微生物剂的方法包括从微生物剂中分析测试数据（例如，在发射波长范围内获得固有荧光值）的步骤。 转化分析试验数据，从而使生物体组内的应变变化最小化。 借助编程的计算机，编码为一组处理指令的多级分类算法对转换的分析测试数据进行操作。 多个级别对应于涉嫌在样品中的微生物剂的分类层级中的不同水平