公开(公告)号：US20230127357A1

公开(公告)日：2023-04-27

申请号：US18046967

申请日：2022-10-17

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： CHRISTOPHER BELISLE ,  JIALI LIAO ,  CALLISTA KATRINA ASUNCION ,  EDWIN LOZANO

IPC: C07K1/16

Abstract: The subject invention pertains to mixed mode chromatography ligands and chromatography matrices suitable for the purification of proteins from biological sources or biological samples. Methods of making chromatography matrices comprising the disclosed ligands and using the disclosed chromatography matrices are also provided.

公开(公告)号：US20220305460A1

公开(公告)日：2022-09-29

申请号：US17696917

申请日：2022-03-17

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： CHRISTOPHER BELISLE ,  JIALI LIAO ,  EDWIN LOZANO

IPC: B01J20/285 ,  B01J20/289 ,  B01J20/30 ,  B01J20/28 ,  C07K1/16 ,  B01D15/38

Abstract: The subject invention pertains to proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic 1,3-droxoisoindolin-2-yl group functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of one, two, three, four, or five atoms between the hydrophobic group and the support matrix.

公开(公告)号：US20240001338A1

公开(公告)日：2024-01-04

申请号：US18360870

申请日：2023-07-28

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： CHRISTOPHER BELISLE ,  JIALI LIAO ,  EDWIN LOZANO

IPC: B01J20/285 ,  B01J20/289 ,  B01J20/30 ,  C07K1/16 ,  B01D15/38 ,  B01J20/28

CPC classification number: B01J20/285 ,  B01J20/289 ,  B01J20/3042 ,  C07K1/165 ,  B01D15/3847 ,  B01D15/3809 ,  B01J20/28085 ,  B01J2220/52

Abstract: The subject invention pertains to proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic 1,3-dioxoisoindolin-2-yl group functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of one, two, three, four, or five atoms between the hydrophobic group and the support matrix.