公开(公告)号：US20120156670A1

公开(公告)日：2012-06-21

申请号：US13296695

申请日：2011-11-15

申请人： Ayesha MAHMOOD ,  Melissa M. HERBST-KRALOVETZ ,  William WARREN

发明人： Ayesha MAHMOOD ,  Melissa M. HERBST-KRALOVETZ ,  William WARREN

IPC分类号： C12Q1/70 ,  C12Q1/02

CPC分类号： C12N5/0683 ,  C12N5/0697 ,  C12N2502/1121 ,  C12N2502/246 ,  C12N2502/28 ,  C12N2503/04 ,  C12N2525/00 ,  C12N2531/00 ,  C12N2533/54 ,  C12N2533/70 ,  G01N33/5044 ,  G01N33/54313 ,  G01N2500/10

摘要： The invention is directed to co-culture systems comprising (i) rotating wall vessel (RWV)-cultured epithelial or differentiated tissue attached to microcarrier beads and (ii) the peripheral tissue equivalent (PTE) module of the MIMIC® system, and to methods of using the co-culture systems for assessing chemical or biological (bacterial or viral) insults. The system models mucosal exposure to chemicals, pathogens or antigen at various sites in the human body. The microcarrier and MIMIC® co-culture approach provides an in vitro co-culture model that simultaneously demonstrates mucosa-mediated antigen presentation and immunogenic responses. Models of the present invention can be used, for example, in assessments of disease pathogenesis and in pharmaceutical development, reproductive physiology, and immunological and toxicological evaluations. Models of the present invention can generate patient-specific localized mucosal immunology using primary cells, resembling the human physiological situation.

摘要翻译： 本发明涉及共培养系统，其包括（i）旋转连接到微载体珠的培养的上皮细胞或分化组织的旋转壁容器（RWV）和（ii）MIMIC系统的外周组织等价物（PTE）模块，以及方法 使用共培养系统来评估化学或生物（细菌或病毒）侮辱。 该系统模拟人体各个部位的化学物质，病原体或抗原的粘膜暴露。 微载体和MIMIC®共培养方法提供体外共培养模型，其同时显示粘膜介导的抗原呈递和免疫原性反应。 本发明的模型可以用于例如疾病发病机理和药物开发，生殖生理学和免疫学和毒理学评估中的评估。 本发明的模型可以使用类似于人类生理情况的原代细胞产生患者特异性局部粘膜免疫学。

公开(公告)号：US20090075282A1

公开(公告)日：2009-03-19

申请号：US12173921

申请日：2008-07-16

申请人： Ayesha MAHMOOD ,  Anatoly KACHURIN ,  William WARREN ,  Russell HIGBEE

发明人： Ayesha MAHMOOD ,  Anatoly KACHURIN ,  William WARREN ,  Russell HIGBEE

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12Q1/02

CPC分类号： A61K9/70 ,  A61K35/17 ,  A61K35/36 ,  C12N5/0688 ,  C12N2502/28 ,  C12N2533/54 ,  G01N33/5082 ,  G01N2800/12

摘要： The present invention comprises artificial tissue constructs that serve as in vitro models of mammalian lung tissue. The artificial tissue constructs of the present invention comprise functionally equivalent in vitro tissue scaffolds that enable immunophysiological function of the lung. The constructs can serve as novel platforms for the study of lung diseases (e.g., interstitial lung diseases, fibrosis, influenza, RSV) as well as smoke- and smoking-related diseases. The artificial tissue constructs of the present invention comprise the two components of alveolar tissue, epithelial and endothelial cell layers.

摘要翻译： 本发明包括用作哺乳动物肺组织的体外模型的人造组织构建体。 本发明的人造组织构建体包括能够实现肺的免疫生理功能的功能上等同的体外组织支架。 这些构建体可以作为研究肺部疾病（例如间质性肺疾病，纤维化，流感，RSV）以及吸烟和吸烟相关疾病的新平台。 本发明的人造组织构建体包含肺泡组织，上皮和内皮细胞层的两个组分。